Urinary alpha1-antichymotrypsin: a biomarker of prion infection.

The occurrence of blood-borne prion transmission incidents calls for identification of potential prion carriers. However, current methods for intravital diagnosis of prion disease rely on invasive tissue biopsies and are unsuitable for large-scale screening. Sensitive biomarkers may help meeting thi...

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Autores principales:	Gino Miele, Harald Seeger, Denis Marino, Ralf Eberhard, Mathias Heikenwalder, Katharina Stoeck, Max Basagni, Richard Knight, Alison Green, Francesca Chianini, Rudolf P Wüthrich, Christoph Hock, Inga Zerr, Adriano Aguzzi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54afcf6458dd4511857b36893341d36a
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spelling	oai:doaj.org-article:54afcf6458dd4511857b36893341d36a2021-11-25T06:18:18ZUrinary alpha1-antichymotrypsin: a biomarker of prion infection.1932-620310.1371/journal.pone.0003870https://doaj.org/article/54afcf6458dd4511857b36893341d36a2008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19057641/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The occurrence of blood-borne prion transmission incidents calls for identification of potential prion carriers. However, current methods for intravital diagnosis of prion disease rely on invasive tissue biopsies and are unsuitable for large-scale screening. Sensitive biomarkers may help meeting this need. Here we scanned the genome for transcripts elevated upon prion infection and encoding secreted proteins. We found that alpha(1)-antichymotrypsin (alpha(1)-ACT) was highly upregulated in brains of scrapie-infected mice. Furthermore, alpha(1)-ACT levels were dramatically increased in urine of patients suffering from sporadic Creutzfeldt-Jakob disease, and increased progressively throughout the disease. Increased alpha(1)-ACT excretion was also found in cases of natural prion disease of animals. Therefore measurement of urinary alpha(1)-ACT levels may be useful for monitoring the efficacy of therapeutic regimens for prion disease, and possibly also for deferring blood and organ donors that may be at risk of transmitting prion infections.Gino MieleHarald SeegerDenis MarinoRalf EberhardMathias HeikenwalderKatharina StoeckMax BasagniRichard KnightAlison GreenFrancesca ChianiniRudolf P WüthrichChristoph HockInga ZerrAdriano AguzziPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3870 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gino Miele Harald Seeger Denis Marino Ralf Eberhard Mathias Heikenwalder Katharina Stoeck Max Basagni Richard Knight Alison Green Francesca Chianini Rudolf P Wüthrich Christoph Hock Inga Zerr Adriano Aguzzi Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
description	The occurrence of blood-borne prion transmission incidents calls for identification of potential prion carriers. However, current methods for intravital diagnosis of prion disease rely on invasive tissue biopsies and are unsuitable for large-scale screening. Sensitive biomarkers may help meeting this need. Here we scanned the genome for transcripts elevated upon prion infection and encoding secreted proteins. We found that alpha(1)-antichymotrypsin (alpha(1)-ACT) was highly upregulated in brains of scrapie-infected mice. Furthermore, alpha(1)-ACT levels were dramatically increased in urine of patients suffering from sporadic Creutzfeldt-Jakob disease, and increased progressively throughout the disease. Increased alpha(1)-ACT excretion was also found in cases of natural prion disease of animals. Therefore measurement of urinary alpha(1)-ACT levels may be useful for monitoring the efficacy of therapeutic regimens for prion disease, and possibly also for deferring blood and organ donors that may be at risk of transmitting prion infections.
format	article
author	Gino Miele Harald Seeger Denis Marino Ralf Eberhard Mathias Heikenwalder Katharina Stoeck Max Basagni Richard Knight Alison Green Francesca Chianini Rudolf P Wüthrich Christoph Hock Inga Zerr Adriano Aguzzi
author_facet	Gino Miele Harald Seeger Denis Marino Ralf Eberhard Mathias Heikenwalder Katharina Stoeck Max Basagni Richard Knight Alison Green Francesca Chianini Rudolf P Wüthrich Christoph Hock Inga Zerr Adriano Aguzzi
author_sort	Gino Miele
title	Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
title_short	Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
title_full	Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
title_fullStr	Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
title_full_unstemmed	Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
title_sort	urinary alpha1-antichymotrypsin: a biomarker of prion infection.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/54afcf6458dd4511857b36893341d36a
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Urinary alpha1-antichymotrypsin: a biomarker of prion infection.

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