Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

Abstract Pre-maturation aging of immature oocytes may adversely affect the fate of an oocyte. Oxidative stress is one of the most detrimental factors affecting oocyte developmental competence and maturation during aging. In this study, experiments were designed to examine whether supplementation of...

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Autores principales:	Li-Feng Liang, Shu-Tao Qi, Ye-Xing Xian, Lin Huang, Xiao-Fang Sun, Wei-Hua Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54b2f55d1b14409c91b97f98884acfe3
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spelling	oai:doaj.org-article:54b2f55d1b14409c91b97f98884acfe32021-12-02T12:30:26ZProtective effect of antioxidants on the pre-maturation aging of mouse oocytes10.1038/s41598-017-01609-32045-2322https://doaj.org/article/54b2f55d1b14409c91b97f98884acfe32017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01609-3https://doaj.org/toc/2045-2322Abstract Pre-maturation aging of immature oocytes may adversely affect the fate of an oocyte. Oxidative stress is one of the most detrimental factors affecting oocyte developmental competence and maturation during aging. In this study, experiments were designed to examine whether supplementation of antioxidants in a culture medium could protect immature mouse oocytes from damages caused by oxidative stress. Mouse oocytes at germinal vesicle stage were prevented from meiosis resumption and cultured in a medium with or without antioxidants for 12–36 h to allow oocytes to undergo aging. After aging, oocytes were cultured for maturation. Nuclear maturation, mitochondria activity, spindle morphology and DNA integrity were examined after maturation. It was found that antioxidants had protective effects on the oocytes in terms of nuclear maturation, functional mitochondria, spindle morphology and DNA integrity. As aging time was prolonged from 12 to 36 h, the protective effect of antioxidants became more obvious. However, as compared with oocytes without aging, it was found that aging significantly inhibited nuclear maturation, impaired mitochondria function, and damaged the spindle and DNA. These results indicate that pre-maturation aging is detrimental to oocytes’ competence to undergo maturation and other cellular activities, and antioxidants can protect oocytes from damages caused by aging.Li-Feng LiangShu-Tao QiYe-Xing XianLin HuangXiao-Fang SunWei-Hua WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li-Feng Liang Shu-Tao Qi Ye-Xing Xian Lin Huang Xiao-Fang Sun Wei-Hua Wang Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
description	Abstract Pre-maturation aging of immature oocytes may adversely affect the fate of an oocyte. Oxidative stress is one of the most detrimental factors affecting oocyte developmental competence and maturation during aging. In this study, experiments were designed to examine whether supplementation of antioxidants in a culture medium could protect immature mouse oocytes from damages caused by oxidative stress. Mouse oocytes at germinal vesicle stage were prevented from meiosis resumption and cultured in a medium with or without antioxidants for 12–36 h to allow oocytes to undergo aging. After aging, oocytes were cultured for maturation. Nuclear maturation, mitochondria activity, spindle morphology and DNA integrity were examined after maturation. It was found that antioxidants had protective effects on the oocytes in terms of nuclear maturation, functional mitochondria, spindle morphology and DNA integrity. As aging time was prolonged from 12 to 36 h, the protective effect of antioxidants became more obvious. However, as compared with oocytes without aging, it was found that aging significantly inhibited nuclear maturation, impaired mitochondria function, and damaged the spindle and DNA. These results indicate that pre-maturation aging is detrimental to oocytes’ competence to undergo maturation and other cellular activities, and antioxidants can protect oocytes from damages caused by aging.
format	article
author	Li-Feng Liang Shu-Tao Qi Ye-Xing Xian Lin Huang Xiao-Fang Sun Wei-Hua Wang
author_facet	Li-Feng Liang Shu-Tao Qi Ye-Xing Xian Lin Huang Xiao-Fang Sun Wei-Hua Wang
author_sort	Li-Feng Liang
title	Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
title_short	Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
title_full	Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
title_fullStr	Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
title_full_unstemmed	Protective effect of antioxidants on the pre-maturation aging of mouse oocytes
title_sort	protective effect of antioxidants on the pre-maturation aging of mouse oocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/54b2f55d1b14409c91b97f98884acfe3
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Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

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