Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition
Abstract MALDI time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used tool for the classification of biological samples. The complex chemical composition of pollen grains leads to highly specific, fingerprint-like mass spectra, with respect to the pollen species. Beyond the species...
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Nature Portfolio
2018
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oai:doaj.org-article:54b6cd56f1064ad1b7c09a765e4378db2021-12-02T15:09:10ZMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition10.1038/s41598-018-34800-12045-2322https://doaj.org/article/54b6cd56f1064ad1b7c09a765e4378db2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34800-1https://doaj.org/toc/2045-2322Abstract MALDI time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used tool for the classification of biological samples. The complex chemical composition of pollen grains leads to highly specific, fingerprint-like mass spectra, with respect to the pollen species. Beyond the species-specific composition, the variances in pollen chemistry can be hierarchically structured, including the level of different populations, of environmental conditions or different genotypes. We demonstrate here the sensitivity of MALDI-TOF MS regarding the adaption of the chemical composition of three Poaceae (grass) pollen for different populations of parent plants by analyzing the mass spectra with partial least squares discriminant analysis (PLS-DA) and principal component analysis (PCA). Thereby, variances in species, population and specific growth conditions of the plants were observed simultaneously. In particular, the chemical pattern revealed by the MALDI spectra enabled discrimination of the different populations of one species. Specifically, the role of environmental changes and their effect on the pollen chemistry of three different grass species is discussed. Analysis of the group formation within the respective populations showed a varying influence of plant genotype on the classification, depending on the species, and permits conclusions regarding the respective rigidity or plasticity towards environmental changes.Sabrina DiehnBoris ZimmermannMurat BağcıoğluStephan SeifertAchim KohlerMikael OhlsonSiri FjellheimSteffen WeidnerJanina KneippNature PortfolioarticleMatrix-assisted Laser Desorption Ionization (MALDI)Grass PollenMALDI SpectraPartial Least Squares Discriminant Analysis (PLS-DA)Growth Condition LevelMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
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DOAJ |
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DOAJ |
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EN |
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Matrix-assisted Laser Desorption Ionization (MALDI) Grass Pollen MALDI Spectra Partial Least Squares Discriminant Analysis (PLS-DA) Growth Condition Level Medicine R Science Q |
| spellingShingle |
Matrix-assisted Laser Desorption Ionization (MALDI) Grass Pollen MALDI Spectra Partial Least Squares Discriminant Analysis (PLS-DA) Growth Condition Level Medicine R Science Q Sabrina Diehn Boris Zimmermann Murat Bağcıoğlu Stephan Seifert Achim Kohler Mikael Ohlson Siri Fjellheim Steffen Weidner Janina Kneipp Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| description |
Abstract MALDI time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used tool for the classification of biological samples. The complex chemical composition of pollen grains leads to highly specific, fingerprint-like mass spectra, with respect to the pollen species. Beyond the species-specific composition, the variances in pollen chemistry can be hierarchically structured, including the level of different populations, of environmental conditions or different genotypes. We demonstrate here the sensitivity of MALDI-TOF MS regarding the adaption of the chemical composition of three Poaceae (grass) pollen for different populations of parent plants by analyzing the mass spectra with partial least squares discriminant analysis (PLS-DA) and principal component analysis (PCA). Thereby, variances in species, population and specific growth conditions of the plants were observed simultaneously. In particular, the chemical pattern revealed by the MALDI spectra enabled discrimination of the different populations of one species. Specifically, the role of environmental changes and their effect on the pollen chemistry of three different grass species is discussed. Analysis of the group formation within the respective populations showed a varying influence of plant genotype on the classification, depending on the species, and permits conclusions regarding the respective rigidity or plasticity towards environmental changes. |
| format |
article |
| author |
Sabrina Diehn Boris Zimmermann Murat Bağcıoğlu Stephan Seifert Achim Kohler Mikael Ohlson Siri Fjellheim Steffen Weidner Janina Kneipp |
| author_facet |
Sabrina Diehn Boris Zimmermann Murat Bağcıoğlu Stephan Seifert Achim Kohler Mikael Ohlson Siri Fjellheim Steffen Weidner Janina Kneipp |
| author_sort |
Sabrina Diehn |
| title |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| title_short |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| title_full |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| title_fullStr |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| title_full_unstemmed |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) shows adaptation of grass pollen composition |
| title_sort |
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) shows adaptation of grass pollen composition |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/54b6cd56f1064ad1b7c09a765e4378db |
| work_keys_str_mv |
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