Experimental Modeling of General Purulent Peritonitis

General purulent peritonitis takes one of the first places in the structure of purulent complications. Despite the im provement of methods of diagnosis and treatment, mortality in postoperative purulent peritonitis remains quite high.Aim of the study: develop a reproducible model of postoperative ge...

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Autores principales:	E. E. Chepurnykh, I. A. Shurygina, T. V. Fadeeva, E. G. Grigoryev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	peritonitis modeling b. fragilis e. coli Science Q
Acceso en línea:	https://doaj.org/article/54bb3fe0380d444088d01cebbf86895a
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spelling	oai:doaj.org-article:54bb3fe0380d444088d01cebbf86895a2021-11-23T06:14:42ZExperimental Modeling of General Purulent Peritonitis2541-94202587-959610.29413/ABS.2019-4.3.15https://doaj.org/article/54bb3fe0380d444088d01cebbf86895a2019-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2093https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596General purulent peritonitis takes one of the first places in the structure of purulent complications. Despite the im provement of methods of diagnosis and treatment, mortality in postoperative purulent peritonitis remains quite high.Aim of the study: develop a reproducible model of postoperative general peritonitis to study the pathophysiological mechanisms of its development and the development of pathogenetically substantiated therapy.Materials and methods. The study was performed on 18 Wistar male rats aged 6 months, weighing 250–300 grams. Animals underwent laparotomy and modeling of postoperative general peritonitis according to the proposed method. Hospital strains of Escherichia coli BRLS 109 and Bacteroides fragilis 109 (strain ISCST1982, registered in Genbank), which we isolated from patients with acute appendicitis, with a total volume of 1.0 ml per animal, were used as infectious agents. Animals were taken out of the experiment under anesthesia with compliance with the rules of euthanasia at 1st, 3rd, 7th days after surgery. For morphological studies, samples of a fragment of the abdominal wall and cecum were taken. Bacteriological studies were performed on the 3rd, 7th day from the beginning of the experiment.Results. It was established that all animals developed peritonitis with the progression of purulent-destructive changes in the abdominal cavity and the development of adhesions, which was confirmed by the data of the morphological method of research. According to the results of bacteriological studies on the 3rd day, exudate infection was detected in all rats (in 100 % of observations). E. coli BLS were isolated in all cases at concentrations of 103–104 CFU/ml. The strain B. fragilis is inoculated in 3 animals at a concentration of 103 CFU/ml, which made 50 %. By the 7th day, only an aerobic E. coli strain of BLS was detected in peritoneal exudate at concentrations of 103 CFU/ml in the complete absence of growth of B. fragilis.Conclusion. We have developed a model of postoperative general peritonitis, which allows us to study the dynamics of the development of the inflammatory process in the abdominal cavity and, in terms of its clinical manifestations, is close to a real human disease. The reproducibility of the model is 100 %, which is confirmed by microbiological and morphological data.E. E. ChepurnykhI. A. ShuryginaT. V. FadeevaE. G. GrigoryevScientific Сentre for Family Health and Human Reproduction Problemsarticleperitonitismodelingb. fragilise. coliScienceQRUActa Biomedica Scientifica, Vol 4, Iss 3, Pp 117-121 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	peritonitis modeling b. fragilis e. coli Science Q
spellingShingle	peritonitis modeling b. fragilis e. coli Science Q E. E. Chepurnykh I. A. Shurygina T. V. Fadeeva E. G. Grigoryev Experimental Modeling of General Purulent Peritonitis
description	General purulent peritonitis takes one of the first places in the structure of purulent complications. Despite the im provement of methods of diagnosis and treatment, mortality in postoperative purulent peritonitis remains quite high.Aim of the study: develop a reproducible model of postoperative general peritonitis to study the pathophysiological mechanisms of its development and the development of pathogenetically substantiated therapy.Materials and methods. The study was performed on 18 Wistar male rats aged 6 months, weighing 250–300 grams. Animals underwent laparotomy and modeling of postoperative general peritonitis according to the proposed method. Hospital strains of Escherichia coli BRLS 109 and Bacteroides fragilis 109 (strain ISCST1982, registered in Genbank), which we isolated from patients with acute appendicitis, with a total volume of 1.0 ml per animal, were used as infectious agents. Animals were taken out of the experiment under anesthesia with compliance with the rules of euthanasia at 1st, 3rd, 7th days after surgery. For morphological studies, samples of a fragment of the abdominal wall and cecum were taken. Bacteriological studies were performed on the 3rd, 7th day from the beginning of the experiment.Results. It was established that all animals developed peritonitis with the progression of purulent-destructive changes in the abdominal cavity and the development of adhesions, which was confirmed by the data of the morphological method of research. According to the results of bacteriological studies on the 3rd day, exudate infection was detected in all rats (in 100 % of observations). E. coli BLS were isolated in all cases at concentrations of 103–104 CFU/ml. The strain B. fragilis is inoculated in 3 animals at a concentration of 103 CFU/ml, which made 50 %. By the 7th day, only an aerobic E. coli strain of BLS was detected in peritoneal exudate at concentrations of 103 CFU/ml in the complete absence of growth of B. fragilis.Conclusion. We have developed a model of postoperative general peritonitis, which allows us to study the dynamics of the development of the inflammatory process in the abdominal cavity and, in terms of its clinical manifestations, is close to a real human disease. The reproducibility of the model is 100 %, which is confirmed by microbiological and morphological data.
format	article
author	E. E. Chepurnykh I. A. Shurygina T. V. Fadeeva E. G. Grigoryev
author_facet	E. E. Chepurnykh I. A. Shurygina T. V. Fadeeva E. G. Grigoryev
author_sort	E. E. Chepurnykh
title	Experimental Modeling of General Purulent Peritonitis
title_short	Experimental Modeling of General Purulent Peritonitis
title_full	Experimental Modeling of General Purulent Peritonitis
title_fullStr	Experimental Modeling of General Purulent Peritonitis
title_full_unstemmed	Experimental Modeling of General Purulent Peritonitis
title_sort	experimental modeling of general purulent peritonitis
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/54bb3fe0380d444088d01cebbf86895a
work_keys_str_mv	AT eechepurnykh experimentalmodelingofgeneralpurulentperitonitis AT iashurygina experimentalmodelingofgeneralpurulentperitonitis AT tvfadeeva experimentalmodelingofgeneralpurulentperitonitis AT eggrigoryev experimentalmodelingofgeneralpurulentperitonitis
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Experimental Modeling of General Purulent Peritonitis

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