The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses
Abstract Background Arabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be eluci...
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2021
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oai:doaj.org-article:54bc969797a449ad9b2a83234ec606c62021-11-28T12:07:23ZThe thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses10.1186/s12870-021-03325-71471-2229https://doaj.org/article/54bc969797a449ad9b2a83234ec606c62021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03325-7https://doaj.org/toc/1471-2229Abstract Background Arabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be elucidated. Results In the present study, encoding sequences of AtPDI1 of wild-type (WT) and double-cysteine-mutants were transformed into an AtPDI1 knockdown Arabidopsis line (pdi), and homozygous transgenic plants named pdi-AtPDI1, pdi-AtPDI1m1 and pdi-AtPDI1m2 were obtained. Compared with the WT and pdi-AtPDI1, the respective germination ratios of pdi-AtPDI1m1 and pdi-AtPDI1m2 were significantly lower under abiotic stresses and exogenous ABA treatment, whereas the highest germination rate was obtained with AtPDI1 overexpression in the WT (WT- AtPDI1). The root length among different lines was consistent with the germination rate; a higher germination rate was observed with a longer root length. When seedlings were treated with salt, drought, cold and high temperature stresses, pdi-AtPDI1m1, pdi-AtPDI1m2 and pdi displayed lower survival rates than WT and AtPDI1 overexpression plants. The transcriptional levels of ABA-responsive genes and genes encoding ROS-quenching enzymes were lower in pdi-AtPDI1m1 and pdi-AtPDI1m2 than in pdi-AtPDI1. Conclusion Taken together, these results clearly suggest that the anti-stress function of AtPDI1 is directly related to the activity of disulfide isomerase.Ying LuLi YuanZhou ZhouMengyu WangXiaoyun WangShizhong ZhangQinghua SunBMCarticleAtPDI1ArabidopsisAbiotic stressABA signalingROS eliminationBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-12 (2021) |
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DOAJ |
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EN |
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AtPDI1 Arabidopsis Abiotic stress ABA signaling ROS elimination Botany QK1-989 |
| spellingShingle |
AtPDI1 Arabidopsis Abiotic stress ABA signaling ROS elimination Botany QK1-989 Ying Lu Li Yuan Zhou Zhou Mengyu Wang Xiaoyun Wang Shizhong Zhang Qinghua Sun The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| description |
Abstract Background Arabidopsis protein disulfide isomerase 1 (AtPDI1) has been demonstrated to have disulfide isomerase activity and to be involved in the stress response. However, whether the anti-stress function is directly related to the activities of thiol-disulfide exchange remains to be elucidated. Results In the present study, encoding sequences of AtPDI1 of wild-type (WT) and double-cysteine-mutants were transformed into an AtPDI1 knockdown Arabidopsis line (pdi), and homozygous transgenic plants named pdi-AtPDI1, pdi-AtPDI1m1 and pdi-AtPDI1m2 were obtained. Compared with the WT and pdi-AtPDI1, the respective germination ratios of pdi-AtPDI1m1 and pdi-AtPDI1m2 were significantly lower under abiotic stresses and exogenous ABA treatment, whereas the highest germination rate was obtained with AtPDI1 overexpression in the WT (WT- AtPDI1). The root length among different lines was consistent with the germination rate; a higher germination rate was observed with a longer root length. When seedlings were treated with salt, drought, cold and high temperature stresses, pdi-AtPDI1m1, pdi-AtPDI1m2 and pdi displayed lower survival rates than WT and AtPDI1 overexpression plants. The transcriptional levels of ABA-responsive genes and genes encoding ROS-quenching enzymes were lower in pdi-AtPDI1m1 and pdi-AtPDI1m2 than in pdi-AtPDI1. Conclusion Taken together, these results clearly suggest that the anti-stress function of AtPDI1 is directly related to the activity of disulfide isomerase. |
| format |
article |
| author |
Ying Lu Li Yuan Zhou Zhou Mengyu Wang Xiaoyun Wang Shizhong Zhang Qinghua Sun |
| author_facet |
Ying Lu Li Yuan Zhou Zhou Mengyu Wang Xiaoyun Wang Shizhong Zhang Qinghua Sun |
| author_sort |
Ying Lu |
| title |
The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| title_short |
The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| title_full |
The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| title_fullStr |
The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| title_full_unstemmed |
The thiol-disulfide exchange activity of AtPDI1 is involved in the response to abiotic stresses |
| title_sort |
thiol-disulfide exchange activity of atpdi1 is involved in the response to abiotic stresses |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/54bc969797a449ad9b2a83234ec606c6 |
| work_keys_str_mv |
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| _version_ |
1718408197692194816 |