Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes

Abstract Background Lung squamous cell carcinoma (LUSC), one of the main pathological types of lung cancer, has led to consequential socioeconomic burden. Ferroptosis is an iron‐dependent form of cell death process with potentials for therapeutic target in various kinds of tumors. However, whether f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiayao Diao, Chao Guo, Lei Liu, Guige Wang, Shanqing Li
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	ferroptosis lung squamous cell carcinoma overall survival prognostic signature Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/54bd4be3f71149b788d9fd9a9fdd354b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:54bd4be3f71149b788d9fd9a9fdd354b
record_format	dspace
spelling	oai:doaj.org-article:54bd4be3f71149b788d9fd9a9fdd354b2021-12-02T02:34:55ZIdentification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes1759-77141759-770610.1111/1759-7714.14195https://doaj.org/article/54bd4be3f71149b788d9fd9a9fdd354b2021-12-01T00:00:00Zhttps://doi.org/10.1111/1759-7714.14195https://doaj.org/toc/1759-7706https://doaj.org/toc/1759-7714Abstract Background Lung squamous cell carcinoma (LUSC), one of the main pathological types of lung cancer, has led to consequential socioeconomic burden. Ferroptosis is an iron‐dependent form of cell death process with potentials for therapeutic target in various kinds of tumors. However, whether ferroptosis‐related genes (FRGs) are associated with the prognosis of LUSC patients is still unclear. The aim of this study was to establish a FRGs‐based signature which could stratify patients with LUSC. Methods The RNA sequencing profiles and corresponding clinical data of LUSC patients were retrieved from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) dataset. A FRG‐based signature was developed using the TCGA‐LUSC cohort and validated in the GEO cohort. Gene set enrichment analysis (GSEA) and analysis of immune cell characteristics were conducted to assess the relationship between FRGs and biological function or immune status. A nomogram based on selected clinical factors and the risk scores which were generated from the FRG‐based signature was developed using the TCGA cohort and validated in the GEO cohort. Results A set of 16 FRGs, significantly associated with overall survival (OS) in the TCGA cohort, was identified and could classify LUSC patients into two risk groups. Kaplan–Meier analysis illustrated that the survival rate of the high‐risk group was significantly lower than the low‐risk group. Assessment and external validation of the signature showed that the survival predictive performance of this signature was adequate. Additionally, multiple pathways and functions were enriched through GSEA and the analysis of immune cell characteristics showed significantly different abundances of immune cells among the two risk groups. Finally, a nomogram integrating the FRG‐based signature and selected clinical factors was also developed and assessed in both the TCGA and GEO cohort. Conclusion This study indicated the association between the FRGs and prognosis of patients with LUSC. Targeting ferroptosis may serve as a novel potential therapeutic alternative for LUSC.Xiayao DiaoChao GuoLei LiuGuige WangShanqing LiWileyarticleferroptosislung squamous cell carcinomaoverall survivalprognostic signatureNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENThoracic Cancer, Vol 12, Iss 23, Pp 3236-3247 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ferroptosis lung squamous cell carcinoma overall survival prognostic signature Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	ferroptosis lung squamous cell carcinoma overall survival prognostic signature Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Xiayao Diao Chao Guo Lei Liu Guige Wang Shanqing Li Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
description	Abstract Background Lung squamous cell carcinoma (LUSC), one of the main pathological types of lung cancer, has led to consequential socioeconomic burden. Ferroptosis is an iron‐dependent form of cell death process with potentials for therapeutic target in various kinds of tumors. However, whether ferroptosis‐related genes (FRGs) are associated with the prognosis of LUSC patients is still unclear. The aim of this study was to establish a FRGs‐based signature which could stratify patients with LUSC. Methods The RNA sequencing profiles and corresponding clinical data of LUSC patients were retrieved from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) dataset. A FRG‐based signature was developed using the TCGA‐LUSC cohort and validated in the GEO cohort. Gene set enrichment analysis (GSEA) and analysis of immune cell characteristics were conducted to assess the relationship between FRGs and biological function or immune status. A nomogram based on selected clinical factors and the risk scores which were generated from the FRG‐based signature was developed using the TCGA cohort and validated in the GEO cohort. Results A set of 16 FRGs, significantly associated with overall survival (OS) in the TCGA cohort, was identified and could classify LUSC patients into two risk groups. Kaplan–Meier analysis illustrated that the survival rate of the high‐risk group was significantly lower than the low‐risk group. Assessment and external validation of the signature showed that the survival predictive performance of this signature was adequate. Additionally, multiple pathways and functions were enriched through GSEA and the analysis of immune cell characteristics showed significantly different abundances of immune cells among the two risk groups. Finally, a nomogram integrating the FRG‐based signature and selected clinical factors was also developed and assessed in both the TCGA and GEO cohort. Conclusion This study indicated the association between the FRGs and prognosis of patients with LUSC. Targeting ferroptosis may serve as a novel potential therapeutic alternative for LUSC.
format	article
author	Xiayao Diao Chao Guo Lei Liu Guige Wang Shanqing Li
author_facet	Xiayao Diao Chao Guo Lei Liu Guige Wang Shanqing Li
author_sort	Xiayao Diao
title	Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
title_short	Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
title_full	Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
title_fullStr	Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
title_full_unstemmed	Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
title_sort	identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/54bd4be3f71149b788d9fd9a9fdd354b
work_keys_str_mv	AT xiayaodiao identificationandvalidationofanindividualizedprognosticsignatureoflungsquamouscellcarcinomabasedonferroptosisrelatedgenes AT chaoguo identificationandvalidationofanindividualizedprognosticsignatureoflungsquamouscellcarcinomabasedonferroptosisrelatedgenes AT leiliu identificationandvalidationofanindividualizedprognosticsignatureoflungsquamouscellcarcinomabasedonferroptosisrelatedgenes AT guigewang identificationandvalidationofanindividualizedprognosticsignatureoflungsquamouscellcarcinomabasedonferroptosisrelatedgenes AT shanqingli identificationandvalidationofanindividualizedprognosticsignatureoflungsquamouscellcarcinomabasedonferroptosisrelatedgenes
_version_	1718402350593343488

Identification and validation of an individualized prognostic signature of lung squamous cell carcinoma based on ferroptosis‐related genes

Ejemplares similares