Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

Dandan Luo,1 Shichao Ruan,1 Aiping Liu,2 Xiangdong Kong,3 In-Seop Lee,3,4 Cen Chen1,5 1College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 2Center for Optoelectronics Materials and Devices, Zhejiang Sci-Tech University, Hangzhou 310018, Peo...

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Autores principales:	Luo DD, Ruan SC, Liu AP, Kong XD, Lee IS, Chen C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	biomimetic apatite neural stem cell coprecipitation adhesion proliferation laminin Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/54c4ed97c2b04fb7b45fb38141d1f8bb
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spelling	oai:doaj.org-article:54c4ed97c2b04fb7b45fb38141d1f8bb2021-12-02T00:04:28ZLaminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells1178-2013https://doaj.org/article/54c4ed97c2b04fb7b45fb38141d1f8bb2018-10-01T00:00:00Zhttps://www.dovepress.com/laminin-functionalized-biomimetic-apatite-to-regulate-the-adhesion-and-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Dandan Luo,1 Shichao Ruan,1 Aiping Liu,2 Xiangdong Kong,3 In-Seop Lee,3,4 Cen Chen1,5 1College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 2Center for Optoelectronics Materials and Devices, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 3College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 4Institute of Natural Sciences, Yonsei University, Seoul 03722, Korea; 5Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, People’s Republic of China Background: Functionalizing biomaterial substrates with biological signals shows promise in regulating neural stem cell (NSC) behaviors through mimicking cellular microenvironment. However, diverse methods for immobilizing biological molecules yields promising results but with many problems. Biomimetic apatite is an excellent carrier due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. Therefore, it may provide a promising alternative in regulating NSC behaviors.Methods: Biomimetic apatite immobilized with the extracellular protein – laminin (LN) was prepared through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing LN. The amount of coprecipitated LN and their release kinetics were examined. The adhesion and proliferation behaviors of NSC on biomimetic apatite immobilized with LN were investigated.Results: The coprecipitation approach provided well retention of LN within biomimetic apatite up to 28 days, and supported the adhesion and proliferation of NSCs without cytotoxicity. For long-term cultivation, NSCs formed neurosphere-like aggregates on non-functionalized biomimetic apatite. A monolayer of proliferated NSCs on biomimetic apatite with coprecipitated LN was observed and even more stable than the positive control of LN coated tissue-culture treated polystyrene (TCP).Conclusion: The simple and reproducible method of coprecipitation suggests that biomimetic apatite is an ideal carrier to functionalize materials with biological molecules for neural-related applications. Keywords: biomimetic apatite, neural stem cell, coprecipitation, adhesion, proliferation, laminin Luo DDRuan SCLiu APKong XDLee ISChen CDove Medical Pressarticlebiomimetic apatiteneural stem cellcoprecipitationadhesionproliferationlamininMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6223-6233 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomimetic apatite neural stem cell coprecipitation adhesion proliferation laminin Medicine (General) R5-920
spellingShingle	biomimetic apatite neural stem cell coprecipitation adhesion proliferation laminin Medicine (General) R5-920 Luo DD Ruan SC Liu AP Kong XD Lee IS Chen C Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
description	Dandan Luo,1 Shichao Ruan,1 Aiping Liu,2 Xiangdong Kong,3 In-Seop Lee,3,4 Cen Chen1,5 1College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 2Center for Optoelectronics Materials and Devices, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 3College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, People’s Republic of China; 4Institute of Natural Sciences, Yonsei University, Seoul 03722, Korea; 5Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, People’s Republic of China Background: Functionalizing biomaterial substrates with biological signals shows promise in regulating neural stem cell (NSC) behaviors through mimicking cellular microenvironment. However, diverse methods for immobilizing biological molecules yields promising results but with many problems. Biomimetic apatite is an excellent carrier due to its non-toxicity, good biocompatibility, biodegradability, and favorable affinity to plenty of molecules. Therefore, it may provide a promising alternative in regulating NSC behaviors.Methods: Biomimetic apatite immobilized with the extracellular protein – laminin (LN) was prepared through coprecipitation process in modified Dulbecco’s phosphate-buffered saline (DPBS) containing LN. The amount of coprecipitated LN and their release kinetics were examined. The adhesion and proliferation behaviors of NSC on biomimetic apatite immobilized with LN were investigated.Results: The coprecipitation approach provided well retention of LN within biomimetic apatite up to 28 days, and supported the adhesion and proliferation of NSCs without cytotoxicity. For long-term cultivation, NSCs formed neurosphere-like aggregates on non-functionalized biomimetic apatite. A monolayer of proliferated NSCs on biomimetic apatite with coprecipitated LN was observed and even more stable than the positive control of LN coated tissue-culture treated polystyrene (TCP).Conclusion: The simple and reproducible method of coprecipitation suggests that biomimetic apatite is an ideal carrier to functionalize materials with biological molecules for neural-related applications. Keywords: biomimetic apatite, neural stem cell, coprecipitation, adhesion, proliferation, laminin
format	article
author	Luo DD Ruan SC Liu AP Kong XD Lee IS Chen C
author_facet	Luo DD Ruan SC Liu AP Kong XD Lee IS Chen C
author_sort	Luo DD
title	Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
title_short	Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
title_full	Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
title_fullStr	Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
title_full_unstemmed	Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
title_sort	laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/54c4ed97c2b04fb7b45fb38141d1f8bb
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Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells

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