Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells

Abstract Misfolding of the cellular prion protein, PrPC, into the amyloidogenic isoform, PrPSc, which forms infectious protein aggregates, the so-called prions, is a key pathogenic event in prion diseases. No pathogens other than prions have been identified to induce misfolding of PrPC into PrPSc an...

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Autores principales:	Hideyuki Hara, Junji Chida, Keiji Uchiyama, Agriani Dini Pasiana, Etsuhisa Takahashi, Hiroshi Kido, Suehiro Sakaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54c57a68787c4beb8f8d7706db3b0d5c
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spelling	oai:doaj.org-article:54c57a68787c4beb8f8d7706db3b0d5c2021-12-02T17:15:36ZNeurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells10.1038/s41598-021-89586-62045-2322https://doaj.org/article/54c57a68787c4beb8f8d7706db3b0d5c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89586-6https://doaj.org/toc/2045-2322Abstract Misfolding of the cellular prion protein, PrPC, into the amyloidogenic isoform, PrPSc, which forms infectious protein aggregates, the so-called prions, is a key pathogenic event in prion diseases. No pathogens other than prions have been identified to induce misfolding of PrPC into PrPSc and propagate infectious prions in infected cells. Here, we found that infection with a neurotropic influenza A virus strain (IAV/WSN) caused misfolding of PrPC into PrPSc and generated infectious prions in mouse neuroblastoma cells through a hit-and-run mechanism. The structural and biochemical characteristics of IAV/WSN-induced PrPSc were different from those of RML and 22L laboratory prions-evoked PrPSc, and the pathogenicity of IAV/WSN-induced prions were also different from that of RML and 22L prions, suggesting IAV/WSN-specific formation of PrPSc and infectious prions. Our current results may open a new avenue for the role of viral infection in misfolding of PrPC into PrPSc and formation of infectious prions.Hideyuki HaraJunji ChidaKeiji UchiyamaAgriani Dini PasianaEtsuhisa TakahashiHiroshi KidoSuehiro SakaguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hideyuki Hara Junji Chida Keiji Uchiyama Agriani Dini Pasiana Etsuhisa Takahashi Hiroshi Kido Suehiro Sakaguchi Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
description	Abstract Misfolding of the cellular prion protein, PrPC, into the amyloidogenic isoform, PrPSc, which forms infectious protein aggregates, the so-called prions, is a key pathogenic event in prion diseases. No pathogens other than prions have been identified to induce misfolding of PrPC into PrPSc and propagate infectious prions in infected cells. Here, we found that infection with a neurotropic influenza A virus strain (IAV/WSN) caused misfolding of PrPC into PrPSc and generated infectious prions in mouse neuroblastoma cells through a hit-and-run mechanism. The structural and biochemical characteristics of IAV/WSN-induced PrPSc were different from those of RML and 22L laboratory prions-evoked PrPSc, and the pathogenicity of IAV/WSN-induced prions were also different from that of RML and 22L prions, suggesting IAV/WSN-specific formation of PrPSc and infectious prions. Our current results may open a new avenue for the role of viral infection in misfolding of PrPC into PrPSc and formation of infectious prions.
format	article
author	Hideyuki Hara Junji Chida Keiji Uchiyama Agriani Dini Pasiana Etsuhisa Takahashi Hiroshi Kido Suehiro Sakaguchi
author_facet	Hideyuki Hara Junji Chida Keiji Uchiyama Agriani Dini Pasiana Etsuhisa Takahashi Hiroshi Kido Suehiro Sakaguchi
author_sort	Hideyuki Hara
title	Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
title_short	Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
title_full	Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
title_fullStr	Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
title_full_unstemmed	Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
title_sort	neurotropic influenza a virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/54c57a68787c4beb8f8d7706db3b0d5c
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Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells

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