Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay

Abstract Blood choline has been proposed as a predictor of acute coronary syndrome (ACS), however different testing procedures might affect the choline concentration because the lysophospholipase D activity of autotaxin (ATX) can convert lysophosphatidylcholine to lysophosphatidic acid (LPA) and cho...

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Autores principales:	Ryunosuke Ohkawa, Makoto Kurano, Noboru Sakai, Tatsuya Kishimoto, Takahiro Nojiri, Koji Igarashi, Shigemi Hosogaya, Yukio Ozaki, Tomotaka Dohi, Katsumi Miyauchi, Hiroyuki Daida, Junken Aoki, Shigeo Okubo, Hitoshi Ikeda, Minoru Tozuka, Yutaka Yatomi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54d0aae38fab4404ba5e3ce2c9e90834
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spelling	oai:doaj.org-article:54d0aae38fab4404ba5e3ce2c9e908342021-12-02T15:08:02ZMeasurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay10.1038/s41598-018-23009-x2045-2322https://doaj.org/article/54d0aae38fab4404ba5e3ce2c9e908342018-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23009-xhttps://doaj.org/toc/2045-2322Abstract Blood choline has been proposed as a predictor of acute coronary syndrome (ACS), however different testing procedures might affect the choline concentration because the lysophospholipase D activity of autotaxin (ATX) can convert lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline in human blood. Although the influences of ATX on LPA levels are well known in vivo and in vitro, those on choline have not been elucidated. Therefore, we established suitable sampling conditions and evaluated the usefulness of plasma choline concentrations as a biomarker for ACS. Serum LPA and choline concentrations dramatically increased after incubation depending on the presence of ATX, while their concentrations in plasma under several conditions were differently modulated. Plasma choline levels in genetically modified mice and healthy human subjects, however, were not influenced by the ATX level in vivo, while the plasma LPA concentrations were associated with ATX. With strict sample preparation, the plasma choline levels did not increase, but actually decreased in ACS patients. Our study revealed that ATX increased the choline concentrations after blood sampling but was not correlated with the choline concentrations in vivo; therefore, strict sample preparation will be necessary to investigate the possible use of choline as a biomarker.Ryunosuke OhkawaMakoto KuranoNoboru SakaiTatsuya KishimotoTakahiro NojiriKoji IgarashiShigemi HosogayaYukio OzakiTomotaka DohiKatsumi MiyauchiHiroyuki DaidaJunken AokiShigeo OkuboHitoshi IkedaMinoru TozukaYutaka YatomiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryunosuke Ohkawa Makoto Kurano Noboru Sakai Tatsuya Kishimoto Takahiro Nojiri Koji Igarashi Shigemi Hosogaya Yukio Ozaki Tomotaka Dohi Katsumi Miyauchi Hiroyuki Daida Junken Aoki Shigeo Okubo Hitoshi Ikeda Minoru Tozuka Yutaka Yatomi Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
description	Abstract Blood choline has been proposed as a predictor of acute coronary syndrome (ACS), however different testing procedures might affect the choline concentration because the lysophospholipase D activity of autotaxin (ATX) can convert lysophosphatidylcholine to lysophosphatidic acid (LPA) and choline in human blood. Although the influences of ATX on LPA levels are well known in vivo and in vitro, those on choline have not been elucidated. Therefore, we established suitable sampling conditions and evaluated the usefulness of plasma choline concentrations as a biomarker for ACS. Serum LPA and choline concentrations dramatically increased after incubation depending on the presence of ATX, while their concentrations in plasma under several conditions were differently modulated. Plasma choline levels in genetically modified mice and healthy human subjects, however, were not influenced by the ATX level in vivo, while the plasma LPA concentrations were associated with ATX. With strict sample preparation, the plasma choline levels did not increase, but actually decreased in ACS patients. Our study revealed that ATX increased the choline concentrations after blood sampling but was not correlated with the choline concentrations in vivo; therefore, strict sample preparation will be necessary to investigate the possible use of choline as a biomarker.
format	article
author	Ryunosuke Ohkawa Makoto Kurano Noboru Sakai Tatsuya Kishimoto Takahiro Nojiri Koji Igarashi Shigemi Hosogaya Yukio Ozaki Tomotaka Dohi Katsumi Miyauchi Hiroyuki Daida Junken Aoki Shigeo Okubo Hitoshi Ikeda Minoru Tozuka Yutaka Yatomi
author_facet	Ryunosuke Ohkawa Makoto Kurano Noboru Sakai Tatsuya Kishimoto Takahiro Nojiri Koji Igarashi Shigemi Hosogaya Yukio Ozaki Tomotaka Dohi Katsumi Miyauchi Hiroyuki Daida Junken Aoki Shigeo Okubo Hitoshi Ikeda Minoru Tozuka Yutaka Yatomi
author_sort	Ryunosuke Ohkawa
title	Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
title_short	Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
title_full	Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
title_fullStr	Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
title_full_unstemmed	Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
title_sort	measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/54d0aae38fab4404ba5e3ce2c9e90834
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Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay

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