Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats

BACKGROUND AND OBJECTIVE: Due to the proximity of epicardial adipose tissue (EAT) to the myocardial tissue, it is considered that EAT be more pathogenic than subcutaneous adipose tissue. The aim of this study was to evaluate the alterations of PGC-1α andUCP1 gene expression in EAT and orexin-A follo...

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Autores principales:	E Moslehi, V Minasian, H Sadeghi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2020
Materias:	aerobic exercise eat obesity orexin-a pgc-1α ucp1. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/54d4068fdac6406a842b5a537379cd5b
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spelling	oai:doaj.org-article:54d4068fdac6406a842b5a537379cd5b2021-11-09T10:15:54ZAlterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats1561-41072251-7170https://doaj.org/article/54d4068fdac6406a842b5a537379cd5b2020-03-01T00:00:00Zhttp://jbums.org/article-1-8761-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Due to the proximity of epicardial adipose tissue (EAT) to the myocardial tissue, it is considered that EAT be more pathogenic than subcutaneous adipose tissue. The aim of this study was to evaluate the alterations of PGC-1α andUCP1 gene expression in EAT and orexin-A following aerobic exercise in high-fat diet induced and obese male wistar rats. METHODS: In this study, 32 male wistar rats aged 6-week and weight of 180-200 g, assigned randomly in: 1) Normal fat diet (NFD), 2) High-fat diet induced obesity (HFDO), 3) Normal fat diet after high-fat diet induced obesity group (HFDO-NFD) and Aerobic exercise group with normal fat diet after high-fat diet induced obesity group (HFDO-AEX). After obesity-induced in HFDO group (8-week diet with 60% fat) and 48 hours after eight weeks of aerobic exercise (60% of maximal training capacity, 4 sessions/week) in other groups, fasting levels of OXA, Lee index, lipid profile, and gene expression of PGC-1α and UCP1 in EAT have been measured. FINDINGS: The results revealed that HFD significantly decreased serum OXA, HDL-c, gene expression of PGC1α and UCP1, also caused a significant increase in Lee index, TG, LDL-c, cholesterol and EAT mass (p≤0.001), but aerobic exercise significantly improved the OXA (34.74%), HDL-c (23.65%), gene expression of PGC-1α and UCP1 (61.28% and 82.67%), lipid profile, EAT mass (76.19%) and Lee index (18.34%) to the normal levels (P≤ 0.001). CONCLUSION: Aerobic exercise by affecting OXA and gene expression of PGC-1α and UCP1 in EAT, probably could reduce the risk factors of cardiovascular diseases due to high-fat diet.E MoslehiV MinasianH SadeghiBabol University of Medical Sciencesarticleaerobic exerciseeatobesityorexin-apgc-1αucp1.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 22, Iss 1, Pp 92-100 (2020)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	aerobic exercise eat obesity orexin-a pgc-1α ucp1. Medicine R Medicine (General) R5-920
spellingShingle	aerobic exercise eat obesity orexin-a pgc-1α ucp1. Medicine R Medicine (General) R5-920 E Moslehi V Minasian H Sadeghi Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
description	BACKGROUND AND OBJECTIVE: Due to the proximity of epicardial adipose tissue (EAT) to the myocardial tissue, it is considered that EAT be more pathogenic than subcutaneous adipose tissue. The aim of this study was to evaluate the alterations of PGC-1α andUCP1 gene expression in EAT and orexin-A following aerobic exercise in high-fat diet induced and obese male wistar rats. METHODS: In this study, 32 male wistar rats aged 6-week and weight of 180-200 g, assigned randomly in: 1) Normal fat diet (NFD), 2) High-fat diet induced obesity (HFDO), 3) Normal fat diet after high-fat diet induced obesity group (HFDO-NFD) and Aerobic exercise group with normal fat diet after high-fat diet induced obesity group (HFDO-AEX). After obesity-induced in HFDO group (8-week diet with 60% fat) and 48 hours after eight weeks of aerobic exercise (60% of maximal training capacity, 4 sessions/week) in other groups, fasting levels of OXA, Lee index, lipid profile, and gene expression of PGC-1α and UCP1 in EAT have been measured. FINDINGS: The results revealed that HFD significantly decreased serum OXA, HDL-c, gene expression of PGC1α and UCP1, also caused a significant increase in Lee index, TG, LDL-c, cholesterol and EAT mass (p≤0.001), but aerobic exercise significantly improved the OXA (34.74%), HDL-c (23.65%), gene expression of PGC-1α and UCP1 (61.28% and 82.67%), lipid profile, EAT mass (76.19%) and Lee index (18.34%) to the normal levels (P≤ 0.001). CONCLUSION: Aerobic exercise by affecting OXA and gene expression of PGC-1α and UCP1 in EAT, probably could reduce the risk factors of cardiovascular diseases due to high-fat diet.
format	article
author	E Moslehi V Minasian H Sadeghi
author_facet	E Moslehi V Minasian H Sadeghi
author_sort	E Moslehi
title	Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
title_short	Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
title_full	Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
title_fullStr	Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
title_full_unstemmed	Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats
title_sort	alterations in pgc-1α and ucp1 gene expression in epicardial aadipose tissue and serum orexin-a following aerobic exercise in high-fat diet induced obesity of male wistar rats
publisher	Babol University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/54d4068fdac6406a842b5a537379cd5b
work_keys_str_mv	AT emoslehi alterationsinpgc1aanducp1geneexpressioninepicardialaadiposetissueandserumorexinafollowingaerobicexerciseinhighfatdietinducedobesityofmalewistarrats AT vminasian alterationsinpgc1aanducp1geneexpressioninepicardialaadiposetissueandserumorexinafollowingaerobicexerciseinhighfatdietinducedobesityofmalewistarrats AT hsadeghi alterationsinpgc1aanducp1geneexpressioninepicardialaadiposetissueandserumorexinafollowingaerobicexerciseinhighfatdietinducedobesityofmalewistarrats
_version_	1718441196411420672

Alterations in PGC-1α and UCP1 Gene Expression in Epicardial Aadipose Tissue and Serum Orexin-A Following Aerobic Exercise in High-Fat Diet Induced Obesity of Male Wistar Rats

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