Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

Qiong-Zhi Gan,1,2 Xin-Yuan Sun,1,2 Poonam Bhadja,1,2 Xiu-Qiong Yao,1,2 Jian-Ming Ouyang1,2 1Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, People’s Republic of...

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Autores principales:	Gan QZ, Sun XY, Bhadja P, Yao XQ, Ouyang JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nano calcium oxalate crystals cell injury crystal adhesion kidney stones Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/54dfd887f22b43458f3d95c36c849c67
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spelling	oai:doaj.org-article:54dfd887f22b43458f3d95c36c849c672021-12-02T02:40:32ZReinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation1178-2013https://doaj.org/article/54dfd887f22b43458f3d95c36c849c672016-06-01T00:00:00Zhttps://www.dovepress.com/reinjury-risk-of-nano-calcium-oxalate-monohydrate-and-calcium-oxalate--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qiong-Zhi Gan,1,2 Xin-Yuan Sun,1,2 Poonam Bhadja,1,2 Xiu-Qiong Yao,1,2 Jian-Ming Ouyang1,2 1Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, People’s Republic of China Background: Renal epithelial cell injury facilitates crystal adhesion to cell surface and serves as a key step in renal stone formation. However, the effects of cell injury on the adhesion of nano-calcium oxalate crystals and the nano-crystal-induced reinjury risk of injured cells remain unclear.Methods: African green monkey renal epithelial (Vero) cells were injured with H2O2 to establish a cell injury model. Cell viability, superoxide dismutase (SOD) activity, malonaldehyde (MDA) content, propidium iodide staining, hematoxylin–eosin staining, reactive oxygen species production, and mitochondrial membrane potential (ΔΨm) were determined to examine cell injury during adhesion. Changes in the surface structure of H2O2-injured cells were assessed through atomic force microscopy. The altered expression of hyaluronan during adhesion was examined through laser scanning confocal microscopy. The adhesion of nano-calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals to Vero cells was observed through scanning electron microscopy. Nano-COM and COD binding was quantitatively determined through inductively coupled plasma emission spectrometry.Results: The expression of hyaluronan on the cell surface was increased during wound healing because of Vero cell injury. The structure and function of the cell membrane were also altered by cell injury; thus, nano-crystal adhesion occurred. The ability of nano-COM to adhere to the injured Vero cells was higher than that of nano-COD crystals. The cell viability, SOD activity, and ΔΨm decreased when nano-crystals attached to the cell surface. By contrast, the MDA content, reactive oxygen species production, and cell death rate increased.Conclusion: Cell injury contributes to crystal adhesion to Vero cell surface. The attached nano-COM and COD crystals can aggravate Vero cell injury. As a consequence, crystal adhesion and aggregation are enhanced. These findings provide further insights into kidney stone formation. Keywords: nano-calcium oxalate crystals, cell injury, crystal adhesion, kidney stonesGan QZSun XYBhadja PYao XQOuyang JMDove Medical PressarticleNano calcium oxalate crystalscell injurycrystal adhesionkidney stonesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2839-2854 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nano calcium oxalate crystals cell injury crystal adhesion kidney stones Medicine (General) R5-920
spellingShingle	Nano calcium oxalate crystals cell injury crystal adhesion kidney stones Medicine (General) R5-920 Gan QZ Sun XY Bhadja P Yao XQ Ouyang JM Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
description	Qiong-Zhi Gan,1,2 Xin-Yuan Sun,1,2 Poonam Bhadja,1,2 Xiu-Qiong Yao,1,2 Jian-Ming Ouyang1,2 1Department of Chemistry, Jinan University, Guangzhou, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, People’s Republic of China Background: Renal epithelial cell injury facilitates crystal adhesion to cell surface and serves as a key step in renal stone formation. However, the effects of cell injury on the adhesion of nano-calcium oxalate crystals and the nano-crystal-induced reinjury risk of injured cells remain unclear.Methods: African green monkey renal epithelial (Vero) cells were injured with H2O2 to establish a cell injury model. Cell viability, superoxide dismutase (SOD) activity, malonaldehyde (MDA) content, propidium iodide staining, hematoxylin–eosin staining, reactive oxygen species production, and mitochondrial membrane potential (ΔΨm) were determined to examine cell injury during adhesion. Changes in the surface structure of H2O2-injured cells were assessed through atomic force microscopy. The altered expression of hyaluronan during adhesion was examined through laser scanning confocal microscopy. The adhesion of nano-calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals to Vero cells was observed through scanning electron microscopy. Nano-COM and COD binding was quantitatively determined through inductively coupled plasma emission spectrometry.Results: The expression of hyaluronan on the cell surface was increased during wound healing because of Vero cell injury. The structure and function of the cell membrane were also altered by cell injury; thus, nano-crystal adhesion occurred. The ability of nano-COM to adhere to the injured Vero cells was higher than that of nano-COD crystals. The cell viability, SOD activity, and ΔΨm decreased when nano-crystals attached to the cell surface. By contrast, the MDA content, reactive oxygen species production, and cell death rate increased.Conclusion: Cell injury contributes to crystal adhesion to Vero cell surface. The attached nano-COM and COD crystals can aggravate Vero cell injury. As a consequence, crystal adhesion and aggregation are enhanced. These findings provide further insights into kidney stone formation. Keywords: nano-calcium oxalate crystals, cell injury, crystal adhesion, kidney stones
format	article
author	Gan QZ Sun XY Bhadja P Yao XQ Ouyang JM
author_facet	Gan QZ Sun XY Bhadja P Yao XQ Ouyang JM
author_sort	Gan QZ
title	Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
title_short	Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
title_full	Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
title_fullStr	Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
title_full_unstemmed	Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
title_sort	reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/54dfd887f22b43458f3d95c36c849c67
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Reinjury risk of nano-calcium oxalate monohydrate and calcium oxalate dihydrate crystals on injured renal epithelial cells: aggravation of crystal adhesion and aggregation

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