Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.

<h4>Background</h4>The cellular reservoirs of Kaposi's sarcoma-associated herpesvirus (KSHV) and the exact nature of the putative KSHV-infected circulating precursor of spindle cells of Kaposi's sarcoma (KS) still remain poorly defined. Because KS spindle cells are thought to b...

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Autores principales:	Silvia Della Bella, Adriano Taddeo, Maria L Calabrò, Lucia Brambilla, Monica Bellinvia, Elisa Bergamo, Mario Clerici, Maria L Villa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54e2b71461674c56b8109eebcf384d05
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spelling	oai:doaj.org-article:54e2b71461674c56b8109eebcf384d052021-11-25T06:13:31ZPeripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.1932-620310.1371/journal.pone.0001520https://doaj.org/article/54e2b71461674c56b8109eebcf384d052008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18231605/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The cellular reservoirs of Kaposi's sarcoma-associated herpesvirus (KSHV) and the exact nature of the putative KSHV-infected circulating precursor of spindle cells of Kaposi's sarcoma (KS) still remain poorly defined. Because KS spindle cells are thought to be of endothelial origin, and because mature endothelial cells do not sustain persistent KSHV-infection, our attention was focalized on circulating hematopoietic precursors able to differentiate into endothelial lineage.<h4>Methods and findings</h4>Late endothelial progenitor cells (late-EPCs) were cultured from the peripheral blood mononuclear cells of 16 patients with classic KS. The presence and load of KSHV genomes were analyzed by real-time polymerase chain reaction in DNA extracted from cells and supernatants of late-EPC cultures obtained from 7 patients. Endothelial colonies cultured from the peripheral blood of KS patients were found to satisfy all requisites to be defined late-EPCs: they appeared from the CD14-negative fraction of adherent cells after 11-26 days of culture, could be serially expanded in vitro, expressed high levels of endothelial antigens but lacked leukocyte markers. Late-EPC cultures were found to harbor KSHV-DNA at variable levels and to retain the virus after multiple passages in cells as well as in supernatants, suggesting that a quote of KSHV lytic infection may spontaneously occur. Lytic phase induction or hypoxia could amplify virus release in supernatants.<h4>Conclusion</h4>Our results suggest that circulating endothelial progenitors from KS patients are KSHV-infected and support viral productive replication and may therefore represent potential virus reservoirs and putative precursors of KS spindle cells.Silvia Della BellaAdriano TaddeoMaria L CalabròLucia BrambillaMonica BellinviaElisa BergamoMario ClericiMaria L VillaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 1, p e1520 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Della Bella Adriano Taddeo Maria L Calabrò Lucia Brambilla Monica Bellinvia Elisa Bergamo Mario Clerici Maria L Villa Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
description	<h4>Background</h4>The cellular reservoirs of Kaposi's sarcoma-associated herpesvirus (KSHV) and the exact nature of the putative KSHV-infected circulating precursor of spindle cells of Kaposi's sarcoma (KS) still remain poorly defined. Because KS spindle cells are thought to be of endothelial origin, and because mature endothelial cells do not sustain persistent KSHV-infection, our attention was focalized on circulating hematopoietic precursors able to differentiate into endothelial lineage.<h4>Methods and findings</h4>Late endothelial progenitor cells (late-EPCs) were cultured from the peripheral blood mononuclear cells of 16 patients with classic KS. The presence and load of KSHV genomes were analyzed by real-time polymerase chain reaction in DNA extracted from cells and supernatants of late-EPC cultures obtained from 7 patients. Endothelial colonies cultured from the peripheral blood of KS patients were found to satisfy all requisites to be defined late-EPCs: they appeared from the CD14-negative fraction of adherent cells after 11-26 days of culture, could be serially expanded in vitro, expressed high levels of endothelial antigens but lacked leukocyte markers. Late-EPC cultures were found to harbor KSHV-DNA at variable levels and to retain the virus after multiple passages in cells as well as in supernatants, suggesting that a quote of KSHV lytic infection may spontaneously occur. Lytic phase induction or hypoxia could amplify virus release in supernatants.<h4>Conclusion</h4>Our results suggest that circulating endothelial progenitors from KS patients are KSHV-infected and support viral productive replication and may therefore represent potential virus reservoirs and putative precursors of KS spindle cells.
format	article
author	Silvia Della Bella Adriano Taddeo Maria L Calabrò Lucia Brambilla Monica Bellinvia Elisa Bergamo Mario Clerici Maria L Villa
author_facet	Silvia Della Bella Adriano Taddeo Maria L Calabrò Lucia Brambilla Monica Bellinvia Elisa Bergamo Mario Clerici Maria L Villa
author_sort	Silvia Della Bella
title	Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
title_short	Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
title_full	Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
title_fullStr	Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
title_full_unstemmed	Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.
title_sort	peripheral blood endothelial progenitors as potential reservoirs of kaposi's sarcoma-associated herpesvirus.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/54e2b71461674c56b8109eebcf384d05
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Peripheral blood endothelial progenitors as potential reservoirs of Kaposi's sarcoma-associated herpesvirus.

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