A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

Nanobodies (Nbs) coupled to organic dyes are increasingly used for super-resolution cell imaging, but producing gene-specific Nbs is time-consuming. Here the authors present a peptide-tag/Nb combination for dSTORM imaging which can be easily adapted to different targets in fixed and live cells.

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Autores principales:	David Virant, Bjoern Traenkle, Julia Maier, Philipp D. Kaiser, Mona Bodenhöfer, Christian Schmees, Ilijana Vojnovic, Borbála Pisak-Lukáts, Ulrike Endesfelder, Ulrich Rothbauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/54f15a1821be44af860b121b4620da77
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spelling	oai:doaj.org-article:54f15a1821be44af860b121b4620da772021-12-02T14:40:31ZA peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging10.1038/s41467-018-03191-22041-1723https://doaj.org/article/54f15a1821be44af860b121b4620da772018-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03191-2https://doaj.org/toc/2041-1723Nanobodies (Nbs) coupled to organic dyes are increasingly used for super-resolution cell imaging, but producing gene-specific Nbs is time-consuming. Here the authors present a peptide-tag/Nb combination for dSTORM imaging which can be easily adapted to different targets in fixed and live cells.David VirantBjoern TraenkleJulia MaierPhilipp D. KaiserMona BodenhöferChristian SchmeesIlijana VojnovicBorbála Pisak-LukátsUlrike EndesfelderUlrich RothbauerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q David Virant Bjoern Traenkle Julia Maier Philipp D. Kaiser Mona Bodenhöfer Christian Schmees Ilijana Vojnovic Borbála Pisak-Lukáts Ulrike Endesfelder Ulrich Rothbauer A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
description	Nanobodies (Nbs) coupled to organic dyes are increasingly used for super-resolution cell imaging, but producing gene-specific Nbs is time-consuming. Here the authors present a peptide-tag/Nb combination for dSTORM imaging which can be easily adapted to different targets in fixed and live cells.
format	article
author	David Virant Bjoern Traenkle Julia Maier Philipp D. Kaiser Mona Bodenhöfer Christian Schmees Ilijana Vojnovic Borbála Pisak-Lukáts Ulrike Endesfelder Ulrich Rothbauer
author_facet	David Virant Bjoern Traenkle Julia Maier Philipp D. Kaiser Mona Bodenhöfer Christian Schmees Ilijana Vojnovic Borbála Pisak-Lukáts Ulrike Endesfelder Ulrich Rothbauer
author_sort	David Virant
title	A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
title_short	A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
title_full	A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
title_fullStr	A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
title_full_unstemmed	A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
title_sort	peptide tag-specific nanobody enables high-quality labeling for dstorm imaging
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/54f15a1821be44af860b121b4620da77
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A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

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