A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
Nanobodies (Nbs) coupled to organic dyes are increasingly used for super-resolution cell imaging, but producing gene-specific Nbs is time-consuming. Here the authors present a peptide-tag/Nb combination for dSTORM imaging which can be easily adapted to different targets in fixed and live cells.
Guardado en:
Autores principales: | David Virant, Bjoern Traenkle, Julia Maier, Philipp D. Kaiser, Mona Bodenhöfer, Christian Schmees, Ilijana Vojnovic, Borbála Pisak-Lukáts, Ulrike Endesfelder, Ulrich Rothbauer |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2018
|
Materias: | |
Acceso en línea: | https://doaj.org/article/54f15a1821be44af860b121b4620da77 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM
por: Felix Wäldchen, et al.
Publicado: (2020) -
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
por: Hansjörg Götzke, et al.
Publicado: (2019) -
An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
por: Hongmin Cai, et al.
Publicado: (2020) -
Evaluation of antidiphtheria toxin nanobodies
por: Ghada H Shaker
Publicado: (2010) -
A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies
por: Xun Chen, et al.
Publicado: (2021)