CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile

Abstract Bacteria that reside in the gastrointestinal tract of healthy humans are essential for our health, sustenance and well-being. About 50–60% of those bacteria have the ability to produce resilient spores that are important for the life cycle in the gut and for host-to-host transmission. A gen...

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Autores principales:	Diogo Martins, Michael A. DiCandia, Aristides L. Mendes, Daniela Wetzel, Shonna M. McBride, Adriano O. Henriques, Mónica Serrano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54f5cc4053574dd1b43cd14fc2b17176
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spelling	oai:doaj.org-article:54f5cc4053574dd1b43cd14fc2b171762021-12-02T18:03:27ZCD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile10.1038/s41598-021-86878-92045-2322https://doaj.org/article/54f5cc4053574dd1b43cd14fc2b171762021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86878-9https://doaj.org/toc/2045-2322Abstract Bacteria that reside in the gastrointestinal tract of healthy humans are essential for our health, sustenance and well-being. About 50–60% of those bacteria have the ability to produce resilient spores that are important for the life cycle in the gut and for host-to-host transmission. A genomic signature for sporulation in the human intestine was recently described, which spans both commensals and pathogens such as Clostridioides difficile and contains several genes of unknown function. We report on the characterization of a signature gene, CD25890, which, as we show is involved in the control of sporulation initiation in C. difficile under certain nutritional conditions. Spo0A is the main regulatory protein controlling entry into sporulation and we show that an in-frame deletion of CD25890 results in increased expression of spo0A per cell and increased sporulation. The effect of CD25890 on spo0A is likely indirect and mediated through repression of the sinRR´ operon. Deletion of the CD25890 gene, however, does not alter the expression of the genes coding for the cytotoxins or the genes involved in biofilm formation. Our results suggest that CD25890 acts to modulate sporulation in response to the nutrients present in the environment.Diogo MartinsMichael A. DiCandiaAristides L. MendesDaniela WetzelShonna M. McBrideAdriano O. HenriquesMónica SerranoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diogo Martins Michael A. DiCandia Aristides L. Mendes Daniela Wetzel Shonna M. McBride Adriano O. Henriques Mónica Serrano CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
description	Abstract Bacteria that reside in the gastrointestinal tract of healthy humans are essential for our health, sustenance and well-being. About 50–60% of those bacteria have the ability to produce resilient spores that are important for the life cycle in the gut and for host-to-host transmission. A genomic signature for sporulation in the human intestine was recently described, which spans both commensals and pathogens such as Clostridioides difficile and contains several genes of unknown function. We report on the characterization of a signature gene, CD25890, which, as we show is involved in the control of sporulation initiation in C. difficile under certain nutritional conditions. Spo0A is the main regulatory protein controlling entry into sporulation and we show that an in-frame deletion of CD25890 results in increased expression of spo0A per cell and increased sporulation. The effect of CD25890 on spo0A is likely indirect and mediated through repression of the sinRR´ operon. Deletion of the CD25890 gene, however, does not alter the expression of the genes coding for the cytotoxins or the genes involved in biofilm formation. Our results suggest that CD25890 acts to modulate sporulation in response to the nutrients present in the environment.
format	article
author	Diogo Martins Michael A. DiCandia Aristides L. Mendes Daniela Wetzel Shonna M. McBride Adriano O. Henriques Mónica Serrano
author_facet	Diogo Martins Michael A. DiCandia Aristides L. Mendes Daniela Wetzel Shonna M. McBride Adriano O. Henriques Mónica Serrano
author_sort	Diogo Martins
title	CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
title_short	CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
title_full	CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
title_fullStr	CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
title_full_unstemmed	CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile
title_sort	cd25890, a conserved protein that modulates sporulation initiation in clostridioides difficile
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/54f5cc4053574dd1b43cd14fc2b17176
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CD25890, a conserved protein that modulates sporulation initiation in Clostridioides difficile

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