A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.

Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LS...

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Autores principales:	Zhaoqiang Wang, Yichen Ding, Sandro Satta, Mehrdad Roustaei, Peng Fei, Tzung K Hsiai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/54f997bb830a4fd296648018a3972e19
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spelling	oai:doaj.org-article:54f997bb830a4fd296648018a3972e192021-12-02T19:57:26ZA hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.1553-734X1553-735810.1371/journal.pcbi.1009175https://doaj.org/article/54f997bb830a4fd296648018a3972e192021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009175https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development.Zhaoqiang WangYichen DingSandro SattaMehrdad RoustaeiPeng FeiTzung K HsiaiPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 7, p e1009175 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Zhaoqiang Wang Yichen Ding Sandro Satta Mehrdad Roustaei Peng Fei Tzung K Hsiai A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
description	Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development.
format	article
author	Zhaoqiang Wang Yichen Ding Sandro Satta Mehrdad Roustaei Peng Fei Tzung K Hsiai
author_facet	Zhaoqiang Wang Yichen Ding Sandro Satta Mehrdad Roustaei Peng Fei Tzung K Hsiai
author_sort	Zhaoqiang Wang
title	A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
title_short	A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
title_full	A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
title_fullStr	A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
title_full_unstemmed	A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
title_sort	hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/54f997bb830a4fd296648018a3972e19
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A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.

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