High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system

Abstract Coral reefs are a complex ecosystem consisting of coral animals and a vast array of associated symbionts including the dinoflagellate Symbiodinium, fungi, viruses and bacteria. Several studies have highlighted the importance of coral-associated bacteria and their fundamental roles in fitnes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wirulda Pootakham, Wuttichai Mhuantong, Thippawan Yoocha, Lalita Putchim, Chutima Sonthirod, Chaiwat Naktang, Nalinee Thongtham, Sithichoke Tangphatsornruang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54fad9d1d71e48a980d7a02810388f26
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:54fad9d1d71e48a980d7a02810388f26
record_format	dspace
spelling	oai:doaj.org-article:54fad9d1d71e48a980d7a02810388f262021-12-02T11:40:59ZHigh resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system10.1038/s41598-017-03139-42045-2322https://doaj.org/article/54fad9d1d71e48a980d7a02810388f262017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03139-4https://doaj.org/toc/2045-2322Abstract Coral reefs are a complex ecosystem consisting of coral animals and a vast array of associated symbionts including the dinoflagellate Symbiodinium, fungi, viruses and bacteria. Several studies have highlighted the importance of coral-associated bacteria and their fundamental roles in fitness and survival of the host animal. The scleractinian coral Porites lutea is one of the dominant reef-builders in the Indo-West Pacific. Currently, very little is known about the composition and structure of bacterial communities across P. lutea reefs. The purpose of this study is twofold: to demonstrate the advantages of using PacBio circular consensus sequencing technology in microbial community studies and to investigate the diversity and structure of P. lutea-associated microbiome in the Indo-Pacific. This is the first metagenomic study of marine environmental samples that utilises the PacBio sequencing system to capture full-length 16S rRNA sequences. We observed geographically distinct coral-associated microbial profiles between samples from the Gulf of Thailand and Andaman Sea. Despite the geographical and environmental impacts on the coral-host interactions, we identified a conserved community of bacteria that were present consistently across diverse reef habitats. Finally, we demonstrated the superior performance of full-length 16S rRNA sequences in resolving taxonomic uncertainty of coral associates at the species level.Wirulda PootakhamWuttichai MhuantongThippawan YoochaLalita PutchimChutima SonthirodChaiwat NaktangNalinee ThongthamSithichoke TangphatsornruangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wirulda Pootakham Wuttichai Mhuantong Thippawan Yoocha Lalita Putchim Chutima Sonthirod Chaiwat Naktang Nalinee Thongtham Sithichoke Tangphatsornruang High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
description	Abstract Coral reefs are a complex ecosystem consisting of coral animals and a vast array of associated symbionts including the dinoflagellate Symbiodinium, fungi, viruses and bacteria. Several studies have highlighted the importance of coral-associated bacteria and their fundamental roles in fitness and survival of the host animal. The scleractinian coral Porites lutea is one of the dominant reef-builders in the Indo-West Pacific. Currently, very little is known about the composition and structure of bacterial communities across P. lutea reefs. The purpose of this study is twofold: to demonstrate the advantages of using PacBio circular consensus sequencing technology in microbial community studies and to investigate the diversity and structure of P. lutea-associated microbiome in the Indo-Pacific. This is the first metagenomic study of marine environmental samples that utilises the PacBio sequencing system to capture full-length 16S rRNA sequences. We observed geographically distinct coral-associated microbial profiles between samples from the Gulf of Thailand and Andaman Sea. Despite the geographical and environmental impacts on the coral-host interactions, we identified a conserved community of bacteria that were present consistently across diverse reef habitats. Finally, we demonstrated the superior performance of full-length 16S rRNA sequences in resolving taxonomic uncertainty of coral associates at the species level.
format	article
author	Wirulda Pootakham Wuttichai Mhuantong Thippawan Yoocha Lalita Putchim Chutima Sonthirod Chaiwat Naktang Nalinee Thongtham Sithichoke Tangphatsornruang
author_facet	Wirulda Pootakham Wuttichai Mhuantong Thippawan Yoocha Lalita Putchim Chutima Sonthirod Chaiwat Naktang Nalinee Thongtham Sithichoke Tangphatsornruang
author_sort	Wirulda Pootakham
title	High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
title_short	High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
title_full	High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
title_fullStr	High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
title_full_unstemmed	High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system
title_sort	high resolution profiling of coral-associated bacterial communities using full-length 16s rrna sequence data from pacbio smrt sequencing system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/54fad9d1d71e48a980d7a02810388f26
work_keys_str_mv	AT wiruldapootakham highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT wuttichaimhuantong highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT thippawanyoocha highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT lalitaputchim highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT chutimasonthirod highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT chaiwatnaktang highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT nalineethongtham highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem AT sithichoketangphatsornruang highresolutionprofilingofcoralassociatedbacterialcommunitiesusingfulllength16srrnasequencedatafrompacbiosmrtsequencingsystem
_version_	1718395460679368704

High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system

Ejemplares similares