Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives

Gastric cancer is one of the most common and deadly cancers worldwide. Screening tests as well as tools for prediction of treatment outcomes and prognosis have been developed, but they have many limitations. The integration of liquid biopsy provided new aspects in screening and diagnosis of gastric...

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Autores principales:	Vasiliki Pisanidou, Panagiotis Apostolou, Georgios Beis, Eleana Hatzidaki, Ioannis Papasotiriou
Formato:	article
Lenguaje:	EN
Publicado:	Karger Publishers 2021
Materias:	gastrointestinal cancer array comparative genomic hybridization next-generation sequencing microarrays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/54feab93207c448a8eacb96fe6cd7c54
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spelling	oai:doaj.org-article:54feab93207c448a8eacb96fe6cd7c542021-12-02T12:40:23ZCancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives1662-657510.1159/000520359https://doaj.org/article/54feab93207c448a8eacb96fe6cd7c542021-11-01T00:00:00Zhttps://www.karger.com/Article/FullText/520359https://doaj.org/toc/1662-6575Gastric cancer is one of the most common and deadly cancers worldwide. Screening tests as well as tools for prediction of treatment outcomes and prognosis have been developed, but they have many limitations. The integration of liquid biopsy provided new aspects in screening and diagnosis of gastric cancer. In the present study, we used different techniques, studying the genetic and epigenetic profile of circulating tumor cells. We aimed to acquire all the available information, compare it with already existing studies, and evaluate the benefit of this approach. A blood sample was isolated from 2 gastric cancer patients at stages III–IV, followed by the isolation of CTCs. The circulating tumor cells were used for array comparative genomic hybridization, next-generation sequencing, and whole gene expression microarrays. Different variants were detected, while the microsatellite instability status was stable in both cases. The tumor mutational burden was low to medium. Gene expression assays revealed that >100 genes were overexpressed compared to noncancer samples. Amplifications of X chromosome were also observed in both cases, by using array comparative genomic hybridization. Although there are several techniques for cancer screening, prediction of therapy outcomes, and prognosis, the application of a complete comprehensive cancer panel, combining the study of variants, fusions, chromosomal abnormalities, and gene expression, is more appropriate. Information provided by the above techniques might contribute in designing more efficient treatment protocols and screening tools. Despite the limitation of samples, the data are encouraging, and further study is needed so that they can be used at clinical level.Vasiliki PisanidouPanagiotis ApostolouGeorgios BeisEleana HatzidakiIoannis PapasotiriouKarger Publishersarticlegastrointestinal cancerarray comparative genomic hybridizationnext-generation sequencingmicroarraysNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCase Reports in Oncology, Vol 14, Iss 3, Pp 1682-1690 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastrointestinal cancer array comparative genomic hybridization next-generation sequencing microarrays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	gastrointestinal cancer array comparative genomic hybridization next-generation sequencing microarrays Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Vasiliki Pisanidou Panagiotis Apostolou Georgios Beis Eleana Hatzidaki Ioannis Papasotiriou Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
description	Gastric cancer is one of the most common and deadly cancers worldwide. Screening tests as well as tools for prediction of treatment outcomes and prognosis have been developed, but they have many limitations. The integration of liquid biopsy provided new aspects in screening and diagnosis of gastric cancer. In the present study, we used different techniques, studying the genetic and epigenetic profile of circulating tumor cells. We aimed to acquire all the available information, compare it with already existing studies, and evaluate the benefit of this approach. A blood sample was isolated from 2 gastric cancer patients at stages III–IV, followed by the isolation of CTCs. The circulating tumor cells were used for array comparative genomic hybridization, next-generation sequencing, and whole gene expression microarrays. Different variants were detected, while the microsatellite instability status was stable in both cases. The tumor mutational burden was low to medium. Gene expression assays revealed that >100 genes were overexpressed compared to noncancer samples. Amplifications of X chromosome were also observed in both cases, by using array comparative genomic hybridization. Although there are several techniques for cancer screening, prediction of therapy outcomes, and prognosis, the application of a complete comprehensive cancer panel, combining the study of variants, fusions, chromosomal abnormalities, and gene expression, is more appropriate. Information provided by the above techniques might contribute in designing more efficient treatment protocols and screening tools. Despite the limitation of samples, the data are encouraging, and further study is needed so that they can be used at clinical level.
format	article
author	Vasiliki Pisanidou Panagiotis Apostolou Georgios Beis Eleana Hatzidaki Ioannis Papasotiriou
author_facet	Vasiliki Pisanidou Panagiotis Apostolou Georgios Beis Eleana Hatzidaki Ioannis Papasotiriou
author_sort	Vasiliki Pisanidou
title	Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
title_short	Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
title_full	Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
title_fullStr	Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
title_full_unstemmed	Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives
title_sort	cancer comprehensive analysis in gastric carcinoma: benefits and new perspectives
publisher	Karger Publishers
publishDate	2021
url	https://doaj.org/article/54feab93207c448a8eacb96fe6cd7c54
work_keys_str_mv	AT vasilikipisanidou cancercomprehensiveanalysisingastriccarcinomabenefitsandnewperspectives AT panagiotisapostolou cancercomprehensiveanalysisingastriccarcinomabenefitsandnewperspectives AT georgiosbeis cancercomprehensiveanalysisingastriccarcinomabenefitsandnewperspectives AT eleanahatzidaki cancercomprehensiveanalysisingastriccarcinomabenefitsandnewperspectives AT ioannispapasotiriou cancercomprehensiveanalysisingastriccarcinomabenefitsandnewperspectives
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Cancer Comprehensive Analysis in Gastric Carcinoma: Benefits and New Perspectives

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