Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines

Seham Elhawary,1 Hala EL-Hefnawy,1 Fatma Alzahraa Mokhtar,1 Mansour Sobeh,2 Eman Mostafa,3 Samir Osman,4,* Mohamed El-Raey5,* 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Agro-BioSciences Research Division, Mohammed VI Polytechnic University, Ben-Guerir 43150,...

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Autores principales:	Elhawary S, EL-Hefnawy H, Alzahraa FA, Sobeh M, Mostafa E, Osman S, El-Raey M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	silver nanoparticles hplc-pda/ms/ms cytotoxic jasminum bladder cancer breast cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5501d9abc49e42d38f9d9747b335fe03
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spelling	oai:doaj.org-article:5501d9abc49e42d38f9d9747b335fe032021-12-02T13:57:54ZGreen Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines1178-2013https://doaj.org/article/5501d9abc49e42d38f9d9747b335fe032020-12-01T00:00:00Zhttps://www.dovepress.com/green-synthesis-of-silver-nanoparticles-using-extract-of-jasminum-offi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Seham Elhawary,1 Hala EL-Hefnawy,1 Fatma Alzahraa Mokhtar,1 Mansour Sobeh,2 Eman Mostafa,3 Samir Osman,4,* Mohamed El-Raey5,* 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Agro-BioSciences Research Division, Mohammed VI Polytechnic University, Ben-Guerir 43150, Morocco; 3Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6 October City, Giza, Egypt; 4Department of Pharmacognosy, Faculty of Pharmacy, Oct. 6 University, Giza, Egypt; 5Phytochemistry and Plant Systematic Department, National Research Centre, Dokki, Cairo, Egypt*These authors contributed equally to this workCorrespondence: Fatma Alzahraa MokhtarDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 12412, EgyptTel +201113154863Email drfatmaalzahraa1950@gmail.comIntroduction: Jasminum officinale L. is a very important medicinal and industrial flowering aromatic plant.Methods: The present study deals with Jasminum officinale L. leaves extract (JOLE) as a reducing and capping agent for the synthesis of silver nanoparticles (AgNPs) by the green pathway. Phenolic profile of the extract was evaluated using HPLC-PDA/MS/MS technique. Jasminum officinale L. leaves extract silver nanoparticles (JOLE-AgNPs) were characterized by ultraviolet light (UV), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), zeta potential and X-ray diffraction (XRD). JOLE-AgNPs were examined for their cytotoxic activities by neutral red uptake assay (NRU) against bladder (5637) and breast cancer (MCF-7) cell lines.Results: HPLC-PDA/MS/MS tentatively identified 51 compounds of different chemical classes. UV spectra showed absorption peak at λmax = 363 nm. The biosynthesized AgNPs were predominantly spherical in shape with an average size of 9.22 nm by TEM. The face cubic center (fcc) nature of silver nanoparticles was proved by XRD diffractogram. JOLE-AgNPs exhibited high cytotoxic activity against 5637 and MCF-7 cell lines compared to the cytotoxic activities of JOLE with IC50 of 13.09 μg/mL and 9.3 μg/mL, respectively.Discussion: The silver nanoparticles formed by Jasminum officinale L. showed high cytotoxic activities against MCF-7 and 5637 cell lines and can be introduced as a new alternative cytotoxic medication.Keywords: silver nanoparticles, HPLC-PDA/MS/MS, cytotoxic, Jasminum, bladder cancer, breast cancerElhawary SEL-Hefnawy HAlzahraa FASobeh MMostafa EOsman SEl-Raey MDove Medical Pressarticlesilver nanoparticleshplc-pda/ms/mscytotoxicjasminumbladder cancerbreast cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 9771-9781 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles hplc-pda/ms/ms cytotoxic jasminum bladder cancer breast cancer Medicine (General) R5-920
spellingShingle	silver nanoparticles hplc-pda/ms/ms cytotoxic jasminum bladder cancer breast cancer Medicine (General) R5-920 Elhawary S EL-Hefnawy H Alzahraa FA Sobeh M Mostafa E Osman S El-Raey M Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
description	Seham Elhawary,1 Hala EL-Hefnawy,1 Fatma Alzahraa Mokhtar,1 Mansour Sobeh,2 Eman Mostafa,3 Samir Osman,4,* Mohamed El-Raey5,* 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt; 2Agro-BioSciences Research Division, Mohammed VI Polytechnic University, Ben-Guerir 43150, Morocco; 3Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6 October City, Giza, Egypt; 4Department of Pharmacognosy, Faculty of Pharmacy, Oct. 6 University, Giza, Egypt; 5Phytochemistry and Plant Systematic Department, National Research Centre, Dokki, Cairo, Egypt*These authors contributed equally to this workCorrespondence: Fatma Alzahraa MokhtarDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 12412, EgyptTel +201113154863Email drfatmaalzahraa1950@gmail.comIntroduction: Jasminum officinale L. is a very important medicinal and industrial flowering aromatic plant.Methods: The present study deals with Jasminum officinale L. leaves extract (JOLE) as a reducing and capping agent for the synthesis of silver nanoparticles (AgNPs) by the green pathway. Phenolic profile of the extract was evaluated using HPLC-PDA/MS/MS technique. Jasminum officinale L. leaves extract silver nanoparticles (JOLE-AgNPs) were characterized by ultraviolet light (UV), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), zeta potential and X-ray diffraction (XRD). JOLE-AgNPs were examined for their cytotoxic activities by neutral red uptake assay (NRU) against bladder (5637) and breast cancer (MCF-7) cell lines.Results: HPLC-PDA/MS/MS tentatively identified 51 compounds of different chemical classes. UV spectra showed absorption peak at λmax = 363 nm. The biosynthesized AgNPs were predominantly spherical in shape with an average size of 9.22 nm by TEM. The face cubic center (fcc) nature of silver nanoparticles was proved by XRD diffractogram. JOLE-AgNPs exhibited high cytotoxic activity against 5637 and MCF-7 cell lines compared to the cytotoxic activities of JOLE with IC50 of 13.09 μg/mL and 9.3 μg/mL, respectively.Discussion: The silver nanoparticles formed by Jasminum officinale L. showed high cytotoxic activities against MCF-7 and 5637 cell lines and can be introduced as a new alternative cytotoxic medication.Keywords: silver nanoparticles, HPLC-PDA/MS/MS, cytotoxic, Jasminum, bladder cancer, breast cancer
format	article
author	Elhawary S EL-Hefnawy H Alzahraa FA Sobeh M Mostafa E Osman S El-Raey M
author_facet	Elhawary S EL-Hefnawy H Alzahraa FA Sobeh M Mostafa E Osman S El-Raey M
author_sort	Elhawary S
title	Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
title_short	Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
title_full	Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
title_fullStr	Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
title_full_unstemmed	Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines
title_sort	green synthesis of silver nanoparticles using extract of jasminum officinal l. leaves and evaluation of cytotoxic activity towards bladder (5637) and breast cancer (mcf-7) cell lines
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/5501d9abc49e42d38f9d9747b335fe03
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Green Synthesis of Silver Nanoparticles Using Extract of Jasminum officinal L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines

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