EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation
ABSTRACT In this study, we have characterized the functional properties of a novel Escherichia coli antigen named EsiB (E. coli secretory immunoglobulin A-binding protein), recently reported to protect mice from sepsis. Gene distribution analysis of a panel of 267 strains representative of different...
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American Society for Microbiology
2013
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oai:doaj.org-article:550876e6f66a44ab97c925a0fed524342021-11-15T15:43:09ZEsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation10.1128/mBio.00206-132150-7511https://doaj.org/article/550876e6f66a44ab97c925a0fed524342013-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00206-13https://doaj.org/toc/2150-7511ABSTRACT In this study, we have characterized the functional properties of a novel Escherichia coli antigen named EsiB (E. coli secretory immunoglobulin A-binding protein), recently reported to protect mice from sepsis. Gene distribution analysis of a panel of 267 strains representative of different E. coli pathotypes revealed that esiB is preferentially associated with extraintestinal strains, while the gene is rarely found in either intestinal or nonpathogenic strains. These findings were supported by the presence of anti-EsiB antibodies in the sera of patients affected by urinary tract infections (UTIs). By solving its crystal structure, we observed that EsiB adopts a superhelical fold composed of Sel1-like repeats (SLRs), a feature often associated with bacterial proteins possessing immunomodulatory functions. Indeed, we found that EsiB interacts with secretory immunoglobulin A (SIgA) through a specific motif identified by an immunocapturing approach. Functional assays showed that EsiB binding to SIgA is likely to interfere with productive FcαRI signaling, by inhibiting both SIgA-induced neutrophil chemotaxis and respiratory burst. Indeed, EsiB hampers SIgA-mediated signaling events by reducing the phosphorylation status of key signal-transducer cytosolic proteins, including mitogen-activated kinases. We propose that the interference with such immune events could contribute to the capacity of the bacterium to avoid clearance by neutrophils, as well as reducing the recruitment of immune cells to the infection site. IMPORTANCE Pathogenic Escherichia coli infections have recently been exacerbated by increasing antibiotic resistance and the number of recurrent contagions. Attempts to develop preventive strategies against E. coli have not been successful, mainly due to the large antigenic and genetic variability of virulence factors, but also due to the complexity of the mechanisms used by the pathogen to evade the immune system. In this work, we elucidated the function of a recently discovered protective antigen, named EsiB, and described its capacity to interact with secretory immunoglobulin A (SIgA) and impair effector functions. This work unravels a novel strategy used by E. coli to subvert the host immune response and avoid neutrophil-dependent clearance.Ilaria PastorelloSilvia Rossi PaccaniRoberto RosiniRossella MatteraMario Ferrer NavarroDunja UrosevBarbara NestaPaola Lo SurdoMariangela Del VecchioValentina RippaIsabella BertoldiDanilo Gomes MorielAlexander J. LaarmanJos A. G. van StrijpXavier DauraMariagrazia PizzaLaura SerinoMarco SorianiAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 4 (2013) |
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DOAJ |
| collection |
DOAJ |
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EN |
| topic |
Microbiology QR1-502 |
| spellingShingle |
Microbiology QR1-502 Ilaria Pastorello Silvia Rossi Paccani Roberto Rosini Rossella Mattera Mario Ferrer Navarro Dunja Urosev Barbara Nesta Paola Lo Surdo Mariangela Del Vecchio Valentina Rippa Isabella Bertoldi Danilo Gomes Moriel Alexander J. Laarman Jos A. G. van Strijp Xavier Daura Mariagrazia Pizza Laura Serino Marco Soriani EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| description |
ABSTRACT In this study, we have characterized the functional properties of a novel Escherichia coli antigen named EsiB (E. coli secretory immunoglobulin A-binding protein), recently reported to protect mice from sepsis. Gene distribution analysis of a panel of 267 strains representative of different E. coli pathotypes revealed that esiB is preferentially associated with extraintestinal strains, while the gene is rarely found in either intestinal or nonpathogenic strains. These findings were supported by the presence of anti-EsiB antibodies in the sera of patients affected by urinary tract infections (UTIs). By solving its crystal structure, we observed that EsiB adopts a superhelical fold composed of Sel1-like repeats (SLRs), a feature often associated with bacterial proteins possessing immunomodulatory functions. Indeed, we found that EsiB interacts with secretory immunoglobulin A (SIgA) through a specific motif identified by an immunocapturing approach. Functional assays showed that EsiB binding to SIgA is likely to interfere with productive FcαRI signaling, by inhibiting both SIgA-induced neutrophil chemotaxis and respiratory burst. Indeed, EsiB hampers SIgA-mediated signaling events by reducing the phosphorylation status of key signal-transducer cytosolic proteins, including mitogen-activated kinases. We propose that the interference with such immune events could contribute to the capacity of the bacterium to avoid clearance by neutrophils, as well as reducing the recruitment of immune cells to the infection site. IMPORTANCE Pathogenic Escherichia coli infections have recently been exacerbated by increasing antibiotic resistance and the number of recurrent contagions. Attempts to develop preventive strategies against E. coli have not been successful, mainly due to the large antigenic and genetic variability of virulence factors, but also due to the complexity of the mechanisms used by the pathogen to evade the immune system. In this work, we elucidated the function of a recently discovered protective antigen, named EsiB, and described its capacity to interact with secretory immunoglobulin A (SIgA) and impair effector functions. This work unravels a novel strategy used by E. coli to subvert the host immune response and avoid neutrophil-dependent clearance. |
| format |
article |
| author |
Ilaria Pastorello Silvia Rossi Paccani Roberto Rosini Rossella Mattera Mario Ferrer Navarro Dunja Urosev Barbara Nesta Paola Lo Surdo Mariangela Del Vecchio Valentina Rippa Isabella Bertoldi Danilo Gomes Moriel Alexander J. Laarman Jos A. G. van Strijp Xavier Daura Mariagrazia Pizza Laura Serino Marco Soriani |
| author_facet |
Ilaria Pastorello Silvia Rossi Paccani Roberto Rosini Rossella Mattera Mario Ferrer Navarro Dunja Urosev Barbara Nesta Paola Lo Surdo Mariangela Del Vecchio Valentina Rippa Isabella Bertoldi Danilo Gomes Moriel Alexander J. Laarman Jos A. G. van Strijp Xavier Daura Mariagrazia Pizza Laura Serino Marco Soriani |
| author_sort |
Ilaria Pastorello |
| title |
EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| title_short |
EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| title_full |
EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| title_fullStr |
EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| title_full_unstemmed |
EsiB, a Novel Pathogenic <named-content content-type="genus-species">Escherichia coli</named-content> Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation |
| title_sort |
esib, a novel pathogenic <named-content content-type="genus-species">escherichia coli</named-content> secretory immunoglobulin a-binding protein impairing neutrophil activation |
| publisher |
American Society for Microbiology |
| publishDate |
2013 |
| url |
https://doaj.org/article/550876e6f66a44ab97c925a0fed52434 |
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