Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker

Handayani F, Rahayu SP. 2017. Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker. Biodiversitas 18: 525-529. Lai is an indigenous germplasm of Kalimantan which has potency to be developed as superior tropical fruit. Lai has attractive fl...

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Autores principales:	FITRI HANDAYANI, SRIWULAN PAMUJI RAHAYU
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	genetic diversity issr lai Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5508ac0bb6754d1db02eeb27dfd76c93
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spelling	oai:doaj.org-article:5508ac0bb6754d1db02eeb27dfd76c932021-11-16T13:52:31ZShort Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker1412-033X2085-472210.13057/biodiv/d180212https://doaj.org/article/5508ac0bb6754d1db02eeb27dfd76c932017-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1661https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Handayani F, Rahayu SP. 2017. Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker. Biodiversitas 18: 525-529. Lai is an indigenous germplasm of Kalimantan which has potency to be developed as superior tropical fruit. Lai has attractive flesh color from yellow to reddish orange which represents the high content of carotene with smooth aroma or odorless fruit, and fruit storage period which longer than durian. Unfortunately, nowadays there is limited scientific information about its genetic diversity as important basic information for genetic conservation and breeding program. Batuah village in Kutai Kartanegara District is one of the center of origin and center of diversity of Lai in East Kalimantan, Indonesia which has many local cultivars with superior traits. The aim of this research was to study the genetic diversity of eight Lai local cultivars of Batuah village using molecular marker of ISSR. The total genomic DNA of eight Lai cultivars was isolated and was then used as template in the PCR amplification reaction using 10 ISSR primers. The result shows that similarity coefficient of eight Lai cultivars ranged between 0,34 and 0,58. Seven cultivars were grouped in the same cluster with 0,44 of similarity coefficient, while Lai Durian was separated in 0,34 of similarity coefficient. Genetically, the most similar existing cultivars are Lai Kuning and Lai Belimbing with the similarity coefficient of 0,58.FITRI HANDAYANISRIWULAN PAMUJI RAHAYUMBI & UNS Soloarticlegenetic diversityissrlaiBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 525-529 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic diversity issr lai Biology (General) QH301-705.5
spellingShingle	genetic diversity issr lai Biology (General) QH301-705.5 FITRI HANDAYANI SRIWULAN PAMUJI RAHAYU Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
description	Handayani F, Rahayu SP. 2017. Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker. Biodiversitas 18: 525-529. Lai is an indigenous germplasm of Kalimantan which has potency to be developed as superior tropical fruit. Lai has attractive flesh color from yellow to reddish orange which represents the high content of carotene with smooth aroma or odorless fruit, and fruit storage period which longer than durian. Unfortunately, nowadays there is limited scientific information about its genetic diversity as important basic information for genetic conservation and breeding program. Batuah village in Kutai Kartanegara District is one of the center of origin and center of diversity of Lai in East Kalimantan, Indonesia which has many local cultivars with superior traits. The aim of this research was to study the genetic diversity of eight Lai local cultivars of Batuah village using molecular marker of ISSR. The total genomic DNA of eight Lai cultivars was isolated and was then used as template in the PCR amplification reaction using 10 ISSR primers. The result shows that similarity coefficient of eight Lai cultivars ranged between 0,34 and 0,58. Seven cultivars were grouped in the same cluster with 0,44 of similarity coefficient, while Lai Durian was separated in 0,34 of similarity coefficient. Genetically, the most similar existing cultivars are Lai Kuning and Lai Belimbing with the similarity coefficient of 0,58.
format	article
author	FITRI HANDAYANI SRIWULAN PAMUJI RAHAYU
author_facet	FITRI HANDAYANI SRIWULAN PAMUJI RAHAYU
author_sort	FITRI HANDAYANI
title	Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
title_short	Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
title_full	Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
title_fullStr	Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
title_full_unstemmed	Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker
title_sort	short communication: assessment of genetic diversity in lai (durio kutejensis) local cultivars of batuah (indonesia) using issr marker
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/5508ac0bb6754d1db02eeb27dfd76c93
work_keys_str_mv	AT fitrihandayani shortcommunicationassessmentofgeneticdiversityinlaiduriokutejensislocalcultivarsofbatuahindonesiausingissrmarker AT sriwulanpamujirahayu shortcommunicationassessmentofgeneticdiversityinlaiduriokutejensislocalcultivarsofbatuahindonesiausingissrmarker
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Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker

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