Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases

Abstract The recent emergence of new fentanyl analogues and synthetic opioids on the drug market poses a global public health threat. However, these compounds cannot typically be identified using existing analytical methods. In this study, we aimed to develop and validate a rapid and sensitive metho...

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Autores principales:	Nan Qin, Min Shen, Ping Xiang, Di Wen, Baohua Shen, Hongxiao Deng, Huosheng Qiang, Fenyun Song, Yan Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5517394b259e4095b137bad485ab9f41
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spelling	oai:doaj.org-article:5517394b259e4095b137bad485ab9f412021-12-02T15:33:11ZDetermination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases10.1038/s41598-020-68348-w2045-2322https://doaj.org/article/5517394b259e4095b137bad485ab9f412020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68348-whttps://doaj.org/toc/2045-2322Abstract The recent emergence of new fentanyl analogues and synthetic opioids on the drug market poses a global public health threat. However, these compounds cannot typically be identified using existing analytical methods. In this study, we aimed to develop and validate a rapid and sensitive method based on ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of 37 fentanyl analogues and novel synthetic opioids in hair samples. Hair samples (20 mg) were extracted by cryogenic grinding in an extraction medium of methanol, acetonitrile, and 2 mmol/L ammonium acetate (pH 5.3). Following centrifugation of the samples, the analytes were separated using a WATERS Acquity UPLC HSS T3 column. The limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.5 to 2.5 pg/mg and from 2 to 5 pg/mg, respectively. The intraday and interday precisions were within 13.32% at LOQ, low, medium, and high levels. The accuracies were within the range of 85.63–116.1%. The extraction recoveries were in the range of 89.42–119.68%, and the matrix effects were within the range of 44.81–119.77%. Furthermore, the method was successfully applied to the detection and quantification of fentanyl and sufentanil in hair samples from two authentic cases. Thus, this method has great potential for detecting fentanyl analogues and novel synthetic opioids in forensic work.Nan QinMin ShenPing XiangDi WenBaohua ShenHongxiao DengHuosheng QiangFenyun SongYan ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nan Qin Min Shen Ping Xiang Di Wen Baohua Shen Hongxiao Deng Huosheng Qiang Fenyun Song Yan Shi Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
description	Abstract The recent emergence of new fentanyl analogues and synthetic opioids on the drug market poses a global public health threat. However, these compounds cannot typically be identified using existing analytical methods. In this study, we aimed to develop and validate a rapid and sensitive method based on ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of 37 fentanyl analogues and novel synthetic opioids in hair samples. Hair samples (20 mg) were extracted by cryogenic grinding in an extraction medium of methanol, acetonitrile, and 2 mmol/L ammonium acetate (pH 5.3). Following centrifugation of the samples, the analytes were separated using a WATERS Acquity UPLC HSS T3 column. The limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.5 to 2.5 pg/mg and from 2 to 5 pg/mg, respectively. The intraday and interday precisions were within 13.32% at LOQ, low, medium, and high levels. The accuracies were within the range of 85.63–116.1%. The extraction recoveries were in the range of 89.42–119.68%, and the matrix effects were within the range of 44.81–119.77%. Furthermore, the method was successfully applied to the detection and quantification of fentanyl and sufentanil in hair samples from two authentic cases. Thus, this method has great potential for detecting fentanyl analogues and novel synthetic opioids in forensic work.
format	article
author	Nan Qin Min Shen Ping Xiang Di Wen Baohua Shen Hongxiao Deng Huosheng Qiang Fenyun Song Yan Shi
author_facet	Nan Qin Min Shen Ping Xiang Di Wen Baohua Shen Hongxiao Deng Huosheng Qiang Fenyun Song Yan Shi
author_sort	Nan Qin
title	Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
title_short	Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
title_full	Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
title_fullStr	Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
title_full_unstemmed	Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases
title_sort	determination of 37 fentanyl analogues and novel synthetic opioids in hair by uhplc-ms/ms and its application to authentic cases
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5517394b259e4095b137bad485ab9f41
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Determination of 37 fentanyl analogues and novel synthetic opioids in hair by UHPLC-MS/MS and its application to authentic cases

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