<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin

ABSTRACT Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions....

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Autores principales:	Mark V. Horton, Chad J. Johnson, John F. Kernien, Tarika D. Patel, Brandon C. Lam, J. Z. Alex Cheong, Jennifer J. Meudt, Dhanansayan Shanmuganayagam, Lindsay R. Kalan, Jeniel E. Nett
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Candida auris biofilm pathogenicity skin porcine sweat Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5524c63f69ea42df84b23327c101ca34
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spelling	oai:doaj.org-article:5524c63f69ea42df84b23327c101ca342021-11-15T15:27:53Z<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin10.1128/mSphere.00910-192379-5042https://doaj.org/article/5524c63f69ea42df84b23327c101ca342020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00910-19https://doaj.org/toc/2379-5042ABSTRACT Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions. We show that C. auris demonstrates a high capacity for biofilm formation in this milieu, well beyond that observed for the most commonly isolated Candida sp., Candida albicans. The C. auris biofilms persist in environmental conditions expected in the hospital setting. To model C. auris skin colonization, we designed an ex vivo porcine skin model. We show that C. auris proliferates on porcine skin in multilayer biofilms. This capacity to thrive in skin niche conditions helps explain the propensity of C. auris to colonize skin, persist on medical devices, and rapidly spread in hospitals. These studies provide clinically relevant tools to further characterize this important growth modality. IMPORTANCE The emerging fungal pathogen Candida auris causes invasive infections and is spreading in hospitals worldwide. Why this species exhibits the capacity to transfer efficiently among patients is unknown. Our findings reveal that C. auris forms high-burden biofilms in conditions mimicking sweat on the skin surface. These adherent biofilm communities persist in environmental conditions expected in the hospital setting. Using a pig skin model, we show that C. auris also forms high-burden biofilm structures on the skin surface. Identification of this mode of growth sheds light on how this recently described pathogen persists in hospital settings and spreads among patients.Mark V. HortonChad J. JohnsonJohn F. KernienTarika D. PatelBrandon C. LamJ. Z. Alex CheongJennifer J. MeudtDhanansayan ShanmuganayagamLindsay R. KalanJeniel E. NettAmerican Society for MicrobiologyarticleCandida aurisbiofilmpathogenicityskinporcinesweatMicrobiologyQR1-502ENmSphere, Vol 5, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida auris biofilm pathogenicity skin porcine sweat Microbiology QR1-502
spellingShingle	Candida auris biofilm pathogenicity skin porcine sweat Microbiology QR1-502 Mark V. Horton Chad J. Johnson John F. Kernien Tarika D. Patel Brandon C. Lam J. Z. Alex Cheong Jennifer J. Meudt Dhanansayan Shanmuganayagam Lindsay R. Kalan Jeniel E. Nett <named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
description	ABSTRACT Emerging pathogen Candida auris causes nosocomial outbreaks of life-threatening invasive candidiasis. It is unclear how this species colonizes skin and spreads in health care facilities. Here, we analyzed C. auris growth in synthetic sweat medium designed to mimic axillary skin conditions. We show that C. auris demonstrates a high capacity for biofilm formation in this milieu, well beyond that observed for the most commonly isolated Candida sp., Candida albicans. The C. auris biofilms persist in environmental conditions expected in the hospital setting. To model C. auris skin colonization, we designed an ex vivo porcine skin model. We show that C. auris proliferates on porcine skin in multilayer biofilms. This capacity to thrive in skin niche conditions helps explain the propensity of C. auris to colonize skin, persist on medical devices, and rapidly spread in hospitals. These studies provide clinically relevant tools to further characterize this important growth modality. IMPORTANCE The emerging fungal pathogen Candida auris causes invasive infections and is spreading in hospitals worldwide. Why this species exhibits the capacity to transfer efficiently among patients is unknown. Our findings reveal that C. auris forms high-burden biofilms in conditions mimicking sweat on the skin surface. These adherent biofilm communities persist in environmental conditions expected in the hospital setting. Using a pig skin model, we show that C. auris also forms high-burden biofilm structures on the skin surface. Identification of this mode of growth sheds light on how this recently described pathogen persists in hospital settings and spreads among patients.
format	article
author	Mark V. Horton Chad J. Johnson John F. Kernien Tarika D. Patel Brandon C. Lam J. Z. Alex Cheong Jennifer J. Meudt Dhanansayan Shanmuganayagam Lindsay R. Kalan Jeniel E. Nett
author_facet	Mark V. Horton Chad J. Johnson John F. Kernien Tarika D. Patel Brandon C. Lam J. Z. Alex Cheong Jennifer J. Meudt Dhanansayan Shanmuganayagam Lindsay R. Kalan Jeniel E. Nett
author_sort	Mark V. Horton
title	<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
title_short	<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
title_full	<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
title_fullStr	<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
title_full_unstemmed	<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin
title_sort	<named-content content-type="genus-species">candida auris</named-content> forms high-burden biofilms in skin niche conditions and on porcine skin
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/5524c63f69ea42df84b23327c101ca34
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<named-content content-type="genus-species">Candida auris</named-content> Forms High-Burden Biofilms in Skin Niche Conditions and on Porcine Skin

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