Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma

Abstract Circular RNAs (circRNAs) are novel biomarkers of various cancers. CircRNAs can sponge miRNAs and regulate target mRNAs, which was called competing endogenous RNAs (ceRNA). This study was designed to identify circRNAs related to patients with clear cell renal cell carcinoma (ccRCC) and the f...

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Autores principales:	Yuwei Zhang, Yuchen Zhang, Yangkun Feng, Nan Zhang, Saisai Chen, Chaoqun Gu, Lei Hu, Jiayi Sheng, Bin Xu, Ninghan Feng
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	CCDC8 ceRNA network circRNA clear cell renal cell carcinoma (ccRCC) has_circ_0001167 prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/553909192f614a119ceb3667ffc8d537
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spelling	oai:doaj.org-article:553909192f614a119ceb3667ffc8d5372021-11-22T09:08:48ZConstruction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma2045-763410.1002/cam4.4311https://doaj.org/article/553909192f614a119ceb3667ffc8d5372021-11-01T00:00:00Zhttps://doi.org/10.1002/cam4.4311https://doaj.org/toc/2045-7634Abstract Circular RNAs (circRNAs) are novel biomarkers of various cancers. CircRNAs can sponge miRNAs and regulate target mRNAs, which was called competing endogenous RNAs (ceRNA). This study was designed to identify circRNAs related to patients with clear cell renal cell carcinoma (ccRCC) and the first to select three independent Gene Expression Omnibus microarrays covering circRNAs, miRNAs, and mRNAs for multiple analyses. The data of clinical cases applied in our study were obtained from The Cancer Genome Atlas. We successfully conducted a circRNA/miRNA/mRNA ceRNA network related to ccRCC patients via R software and Cytoscape including 8 circRNAs, 6 miRNAs, and 49 mRNAs. The prognosis‐associated subnet covered 8 circRNAs, 6 miRNAs, and 22 mRNAs. Quantitative real‐time PCR was applied to measure our prediction in three renal cell lines and 23 pairs of tissues. Small interfering RNA targeting the back‐splice region of hsa_circ_0001167 was further implied to confirm the regulation. Ultimately, hsa_circ_0001167/hsa‐miR‐595/CCDC8 regulatory axis was identified in this study, which may serve as prognostic indicators. Lower levels of hsa_circ_0001167 and CCDC8 were potentially correlated with worse patient survival.Yuwei ZhangYuchen ZhangYangkun FengNan ZhangSaisai ChenChaoqun GuLei HuJiayi ShengBin XuNinghan FengWileyarticleCCDC8ceRNA networkcircRNAclear cell renal cell carcinoma (ccRCC)has_circ_0001167prognosisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Medicine, Vol 10, Iss 22, Pp 8210-8221 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CCDC8 ceRNA network circRNA clear cell renal cell carcinoma (ccRCC) has_circ_0001167 prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	CCDC8 ceRNA network circRNA clear cell renal cell carcinoma (ccRCC) has_circ_0001167 prognosis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Yuwei Zhang Yuchen Zhang Yangkun Feng Nan Zhang Saisai Chen Chaoqun Gu Lei Hu Jiayi Sheng Bin Xu Ninghan Feng Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
description	Abstract Circular RNAs (circRNAs) are novel biomarkers of various cancers. CircRNAs can sponge miRNAs and regulate target mRNAs, which was called competing endogenous RNAs (ceRNA). This study was designed to identify circRNAs related to patients with clear cell renal cell carcinoma (ccRCC) and the first to select three independent Gene Expression Omnibus microarrays covering circRNAs, miRNAs, and mRNAs for multiple analyses. The data of clinical cases applied in our study were obtained from The Cancer Genome Atlas. We successfully conducted a circRNA/miRNA/mRNA ceRNA network related to ccRCC patients via R software and Cytoscape including 8 circRNAs, 6 miRNAs, and 49 mRNAs. The prognosis‐associated subnet covered 8 circRNAs, 6 miRNAs, and 22 mRNAs. Quantitative real‐time PCR was applied to measure our prediction in three renal cell lines and 23 pairs of tissues. Small interfering RNA targeting the back‐splice region of hsa_circ_0001167 was further implied to confirm the regulation. Ultimately, hsa_circ_0001167/hsa‐miR‐595/CCDC8 regulatory axis was identified in this study, which may serve as prognostic indicators. Lower levels of hsa_circ_0001167 and CCDC8 were potentially correlated with worse patient survival.
format	article
author	Yuwei Zhang Yuchen Zhang Yangkun Feng Nan Zhang Saisai Chen Chaoqun Gu Lei Hu Jiayi Sheng Bin Xu Ninghan Feng
author_facet	Yuwei Zhang Yuchen Zhang Yangkun Feng Nan Zhang Saisai Chen Chaoqun Gu Lei Hu Jiayi Sheng Bin Xu Ninghan Feng
author_sort	Yuwei Zhang
title	Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
title_short	Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
title_full	Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
title_fullStr	Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
title_full_unstemmed	Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma
title_sort	construction of circrna‐based cerna network and its prognosis‐associated subnet of clear cell renal cell carcinoma
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/553909192f614a119ceb3667ffc8d537
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Construction of circRNA‐based ceRNA network and its prognosis‐associated subnet of clear cell renal cell carcinoma

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