Prematurity alters the progenitor cell program of the upper respiratory tract of neonates
Abstract The impact of prematurity on human development and neonatal diseases, such as bronchopulmonary dysplasia, has been widely reported. However, little is known about the effects of prematurity on the programs of stem cell self-renewal and differentiation of the upper respiratory epithelium, wh...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/553bcca3e5344c2da2c372b094691afd |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:553bcca3e5344c2da2c372b094691afd |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:553bcca3e5344c2da2c372b094691afd2021-12-02T14:49:18ZPrematurity alters the progenitor cell program of the upper respiratory tract of neonates10.1038/s41598-021-90093-x2045-2322https://doaj.org/article/553bcca3e5344c2da2c372b094691afd2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90093-xhttps://doaj.org/toc/2045-2322Abstract The impact of prematurity on human development and neonatal diseases, such as bronchopulmonary dysplasia, has been widely reported. However, little is known about the effects of prematurity on the programs of stem cell self-renewal and differentiation of the upper respiratory epithelium, which is key for adaptation to neonatal life. We developed a minimally invasive methodology for isolation of neonatal basal cells from nasopharyngeal (NP) aspirates and performed functional analysis in organotypic cultures to address this issue. We show that preterm NP progenitors have a markedly distinct molecular signature of abnormal proliferation and mitochondria quality control compared to term progenitors. Preterm progenitors had lower oxygen consumption at baseline and were unable to ramp up consumption to the levels of term cells when challenged. Although they formed a mucociliary epithelium, ciliary function tended to decline in premature cells as they differentiated, compared to term cells. Together, these differences suggested increased sensitivity of preterm progenitors to environmental stressors under non-homeostatic conditions.Jessica E. ShuiWei WangHelu LiuAnna StepanovaGrace LiaoJun QianXingbin AiVadim TenJining LuWellington V. CardosoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Jessica E. Shui Wei Wang Helu Liu Anna Stepanova Grace Liao Jun Qian Xingbin Ai Vadim Ten Jining Lu Wellington V. Cardoso Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| description |
Abstract The impact of prematurity on human development and neonatal diseases, such as bronchopulmonary dysplasia, has been widely reported. However, little is known about the effects of prematurity on the programs of stem cell self-renewal and differentiation of the upper respiratory epithelium, which is key for adaptation to neonatal life. We developed a minimally invasive methodology for isolation of neonatal basal cells from nasopharyngeal (NP) aspirates and performed functional analysis in organotypic cultures to address this issue. We show that preterm NP progenitors have a markedly distinct molecular signature of abnormal proliferation and mitochondria quality control compared to term progenitors. Preterm progenitors had lower oxygen consumption at baseline and were unable to ramp up consumption to the levels of term cells when challenged. Although they formed a mucociliary epithelium, ciliary function tended to decline in premature cells as they differentiated, compared to term cells. Together, these differences suggested increased sensitivity of preterm progenitors to environmental stressors under non-homeostatic conditions. |
| format |
article |
| author |
Jessica E. Shui Wei Wang Helu Liu Anna Stepanova Grace Liao Jun Qian Xingbin Ai Vadim Ten Jining Lu Wellington V. Cardoso |
| author_facet |
Jessica E. Shui Wei Wang Helu Liu Anna Stepanova Grace Liao Jun Qian Xingbin Ai Vadim Ten Jining Lu Wellington V. Cardoso |
| author_sort |
Jessica E. Shui |
| title |
Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| title_short |
Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| title_full |
Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| title_fullStr |
Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| title_full_unstemmed |
Prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| title_sort |
prematurity alters the progenitor cell program of the upper respiratory tract of neonates |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/553bcca3e5344c2da2c372b094691afd |
| work_keys_str_mv |
AT jessicaeshui prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT weiwang prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT heluliu prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT annastepanova prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT graceliao prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT junqian prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT xingbinai prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT vadimten prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT jininglu prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates AT wellingtonvcardoso prematurityalterstheprogenitorcellprogramoftheupperrespiratorytractofneonates |
| _version_ |
1718389508849795072 |