[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts

Abstract Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft survival. We studied [18F]-sodium fluoride ([18F]-NaF) imaging of microcalcifications in donor kidneys (n = 7) and explanted...

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Autores principales:	Stan Benjamens, Ines F. Antunes, Jan-Luuk Hillebrands, Melanie Reijrink, Marian L. C. Bulthuis, Stefan P. Berger, Cyril Moers, Martin H. de Borst, Riemer H. J. A. Slart, Robert A. Pol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/553e895bc15b438592000260d4502b43
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spelling	oai:doaj.org-article:553e895bc15b438592000260d4502b432021-12-02T11:50:30Z[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts10.1038/s41598-021-81144-42045-2322https://doaj.org/article/553e895bc15b438592000260d4502b432021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81144-4https://doaj.org/toc/2045-2322Abstract Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft survival. We studied [18F]-sodium fluoride ([18F]-NaF) imaging of microcalcifications in donor kidneys (n = 7) and explanted kidney allografts (n = 13). Three µm paraffin-embedded serial sections were used for histological evaluation of calcification (Alizarin Red; Von Kossa staining) and ex-vivo [18F]-NaF autoradiography. The images were fused to evaluate if microcalcification areas corresponded with [18F]-NaF uptake areas. Based on histological analyses, tubulointerstitial and glomerular microcalcifications were present in 19/20 and 7/20 samples, respectively. Using autoradiography, [18F]-NaF uptake was found in 19/20 samples, with significantly more tracer activity in kidney allograft compared to deceased donor kidney samples (p = 0.019). Alizarin Red staining of active microcalcifications demonstrated good correlation (Spearman’s rho of 0.81, p < 0.001) and Von Kossa staining of consolidated calcifications demonstrated significant but weak correlation (0.62, p = 0.003) with [18F]-NaF activity. This correlation between ex-vivo [18F]-NaF uptake and histology-proven microcalcifications, is the first step towards an imaging method to identify microcalcifications in active nephrocalcinosis. This may lead to better understanding of the etiology of microcalcifications and its impact on kidney transplant function.Stan BenjamensInes F. AntunesJan-Luuk HillebrandsMelanie ReijrinkMarian L. C. BulthuisStefan P. BergerCyril MoersMartin H. de BorstRiemer H. J. A. SlartRobert A. PolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stan Benjamens Ines F. Antunes Jan-Luuk Hillebrands Melanie Reijrink Marian L. C. Bulthuis Stefan P. Berger Cyril Moers Martin H. de Borst Riemer H. J. A. Slart Robert A. Pol [18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
description	Abstract Nephrocalcinosis is present in up to 43% of kidney allograft biopsies at one-year after transplantation and is associated with inferior graft function and poor graft survival. We studied [18F]-sodium fluoride ([18F]-NaF) imaging of microcalcifications in donor kidneys (n = 7) and explanted kidney allografts (n = 13). Three µm paraffin-embedded serial sections were used for histological evaluation of calcification (Alizarin Red; Von Kossa staining) and ex-vivo [18F]-NaF autoradiography. The images were fused to evaluate if microcalcification areas corresponded with [18F]-NaF uptake areas. Based on histological analyses, tubulointerstitial and glomerular microcalcifications were present in 19/20 and 7/20 samples, respectively. Using autoradiography, [18F]-NaF uptake was found in 19/20 samples, with significantly more tracer activity in kidney allograft compared to deceased donor kidney samples (p = 0.019). Alizarin Red staining of active microcalcifications demonstrated good correlation (Spearman’s rho of 0.81, p < 0.001) and Von Kossa staining of consolidated calcifications demonstrated significant but weak correlation (0.62, p = 0.003) with [18F]-NaF activity. This correlation between ex-vivo [18F]-NaF uptake and histology-proven microcalcifications, is the first step towards an imaging method to identify microcalcifications in active nephrocalcinosis. This may lead to better understanding of the etiology of microcalcifications and its impact on kidney transplant function.
format	article
author	Stan Benjamens Ines F. Antunes Jan-Luuk Hillebrands Melanie Reijrink Marian L. C. Bulthuis Stefan P. Berger Cyril Moers Martin H. de Borst Riemer H. J. A. Slart Robert A. Pol
author_facet	Stan Benjamens Ines F. Antunes Jan-Luuk Hillebrands Melanie Reijrink Marian L. C. Bulthuis Stefan P. Berger Cyril Moers Martin H. de Borst Riemer H. J. A. Slart Robert A. Pol
author_sort	Stan Benjamens
title	[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
title_short	[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
title_full	[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
title_fullStr	[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
title_full_unstemmed	[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
title_sort	[18f]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/553e895bc15b438592000260d4502b43
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[18F]-sodium fluoride autoradiography imaging of nephrocalcinosis in donor kidneys and explanted kidney allografts

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