Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells

Abstract The objective of this study was to compare the effect of contrasting sources of dietary n-6 and n-3 PUFA on expression of genes related to lipid metabolism in dairy cows. During 63 days, fifteen lactating cows were assigned to a control or basal diet containing no added lipid (n = 5 cows);...

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Autores principales:	Einar Vargas-Bello-Pérez, Nathaly Cancino-Padilla, Carolina Geldsetzer-Mendoza, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime Romero
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5541ce7277e34c5cb188e39e8ae2c420
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spelling	oai:doaj.org-article:5541ce7277e34c5cb188e39e8ae2c4202021-12-02T19:12:25ZEffects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells10.1038/s41598-020-71930-x2045-2322https://doaj.org/article/5541ce7277e34c5cb188e39e8ae2c4202020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-71930-xhttps://doaj.org/toc/2045-2322Abstract The objective of this study was to compare the effect of contrasting sources of dietary n-6 and n-3 PUFA on expression of genes related to lipid metabolism in dairy cows. During 63 days, fifteen lactating cows were assigned to a control or basal diet containing no added lipid (n = 5 cows); and treatment diets supplemented with SO (n = 5 cows; unrefined soybean oil; 2.9% of DM) or FO (n = 5 cows; fish oil manufactured from salmon oil; 2.9% of DM). Plasma for fatty acid (FA) analysis and milk somatic cells (MSC) were obtained from all cows at the beginning of the study (day 0) and on days 21, 42 and 63. Plasma was used to determine FA transport dynamics. Compared with control and FO, plasma from SO had increased contents of C18:1 cis-9, C18:1 trans-11, C18:2 cis-9, trans-11 and total monounsaturated FA. On the other hand, compared with control and SO, FO increased plasma contents of C20:3 n-3, C20:3 n-6, C20:4 n-6, C20:5 n-3, C22:6 n-3 and total polyunsaturated FA. Moreover, plasma C18:3 n-3 and C20:5 n-3 increased over time for all diets. Compared with control, SO downregulated ACACA, INSIG1, and DGAT1, whereas FO downregulated ACACA, PPARGC1, LPIN1 and FABP3 on day 63, in MSC. At different time-points, SO and FO downregulated genes related to synthesis and intracellular transport of FA, synthesis of triglycerides, and transcription factors.Einar Vargas-Bello-PérezNathaly Cancino-PadillaCarolina Geldsetzer-MendozaMaría Sol MoralesHeidi LeskinenPhilip C. GarnsworthyJuan J. LoorJaime RomeroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Einar Vargas-Bello-Pérez Nathaly Cancino-Padilla Carolina Geldsetzer-Mendoza María Sol Morales Heidi Leskinen Philip C. Garnsworthy Juan J. Loor Jaime Romero Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
description	Abstract The objective of this study was to compare the effect of contrasting sources of dietary n-6 and n-3 PUFA on expression of genes related to lipid metabolism in dairy cows. During 63 days, fifteen lactating cows were assigned to a control or basal diet containing no added lipid (n = 5 cows); and treatment diets supplemented with SO (n = 5 cows; unrefined soybean oil; 2.9% of DM) or FO (n = 5 cows; fish oil manufactured from salmon oil; 2.9% of DM). Plasma for fatty acid (FA) analysis and milk somatic cells (MSC) were obtained from all cows at the beginning of the study (day 0) and on days 21, 42 and 63. Plasma was used to determine FA transport dynamics. Compared with control and FO, plasma from SO had increased contents of C18:1 cis-9, C18:1 trans-11, C18:2 cis-9, trans-11 and total monounsaturated FA. On the other hand, compared with control and SO, FO increased plasma contents of C20:3 n-3, C20:3 n-6, C20:4 n-6, C20:5 n-3, C22:6 n-3 and total polyunsaturated FA. Moreover, plasma C18:3 n-3 and C20:5 n-3 increased over time for all diets. Compared with control, SO downregulated ACACA, INSIG1, and DGAT1, whereas FO downregulated ACACA, PPARGC1, LPIN1 and FABP3 on day 63, in MSC. At different time-points, SO and FO downregulated genes related to synthesis and intracellular transport of FA, synthesis of triglycerides, and transcription factors.
format	article
author	Einar Vargas-Bello-Pérez Nathaly Cancino-Padilla Carolina Geldsetzer-Mendoza María Sol Morales Heidi Leskinen Philip C. Garnsworthy Juan J. Loor Jaime Romero
author_facet	Einar Vargas-Bello-Pérez Nathaly Cancino-Padilla Carolina Geldsetzer-Mendoza María Sol Morales Heidi Leskinen Philip C. Garnsworthy Juan J. Loor Jaime Romero
author_sort	Einar Vargas-Bello-Pérez
title	Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
title_short	Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
title_full	Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
title_fullStr	Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
title_full_unstemmed	Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
title_sort	effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5541ce7277e34c5cb188e39e8ae2c420
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Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells

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