The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures

Abstract Citrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Teresa Faddetta, Loredana Abbate, Pasquale Alibrandi, Walter Arancio, Davide Siino, Francesco Strati, Carlotta De Filippo, Sergio Fatta Del Bosco, Francesco Carimi, Anna Maria Puglia, Massimiliano Cardinale, Giuseppe Gallo, Francesco Mercati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55473990ceb741aba8f4828584f3bef8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:55473990ceb741aba8f4828584f3bef8
record_format	dspace
spelling	oai:doaj.org-article:55473990ceb741aba8f4828584f3bef82021-12-02T18:17:53ZThe endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures10.1038/s41598-021-86399-52045-2322https://doaj.org/article/55473990ceb741aba8f4828584f3bef82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86399-5https://doaj.org/toc/2045-2322Abstract Citrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging to Aspergillus, Quambalaria and Bjerkandera genera—and bacteria—belonging to Staphylococcus genus—were characterized for indoleacetic acid production and phosphate solubilization. Next Generation Sequencing based approaches were then used to characterize the endophytic bacterial and fungal microbiota structures of surface-sterilized C. limon seeds and of shoots obtained under aseptic conditions from in vitro growing seedlings regenerated from surface-sterilized seeds. This analysis highlighted that Cutibacterium and Acinetobacter were the most abundant bacterial genera in both seeds and shoots, while Cladosporium and Debaryomyces were the most abundant fungal genera in seeds and shoots, respectively. The localization of bacterial endophytes in seed and shoot tissues was revealed by Fluorescence In Situ Hybridization coupled with Confocal Laser Scanning Microscopy revealing vascular bundle colonization. Thus, these results highlighted for the first time the structures of endophytic microbiota of C. limon seeds and the transmission to shoots, corroborating the idea of a vertical transmission of plant microbiota and suggesting its crucial role in seed germination and plant development.Teresa FaddettaLoredana AbbatePasquale AlibrandiWalter ArancioDavide SiinoFrancesco StratiCarlotta De FilippoSergio Fatta Del BoscoFrancesco CarimiAnna Maria PugliaMassimiliano CardinaleGiuseppe GalloFrancesco MercatiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teresa Faddetta Loredana Abbate Pasquale Alibrandi Walter Arancio Davide Siino Francesco Strati Carlotta De Filippo Sergio Fatta Del Bosco Francesco Carimi Anna Maria Puglia Massimiliano Cardinale Giuseppe Gallo Francesco Mercati The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
description	Abstract Citrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging to Aspergillus, Quambalaria and Bjerkandera genera—and bacteria—belonging to Staphylococcus genus—were characterized for indoleacetic acid production and phosphate solubilization. Next Generation Sequencing based approaches were then used to characterize the endophytic bacterial and fungal microbiota structures of surface-sterilized C. limon seeds and of shoots obtained under aseptic conditions from in vitro growing seedlings regenerated from surface-sterilized seeds. This analysis highlighted that Cutibacterium and Acinetobacter were the most abundant bacterial genera in both seeds and shoots, while Cladosporium and Debaryomyces were the most abundant fungal genera in seeds and shoots, respectively. The localization of bacterial endophytes in seed and shoot tissues was revealed by Fluorescence In Situ Hybridization coupled with Confocal Laser Scanning Microscopy revealing vascular bundle colonization. Thus, these results highlighted for the first time the structures of endophytic microbiota of C. limon seeds and the transmission to shoots, corroborating the idea of a vertical transmission of plant microbiota and suggesting its crucial role in seed germination and plant development.
format	article
author	Teresa Faddetta Loredana Abbate Pasquale Alibrandi Walter Arancio Davide Siino Francesco Strati Carlotta De Filippo Sergio Fatta Del Bosco Francesco Carimi Anna Maria Puglia Massimiliano Cardinale Giuseppe Gallo Francesco Mercati
author_facet	Teresa Faddetta Loredana Abbate Pasquale Alibrandi Walter Arancio Davide Siino Francesco Strati Carlotta De Filippo Sergio Fatta Del Bosco Francesco Carimi Anna Maria Puglia Massimiliano Cardinale Giuseppe Gallo Francesco Mercati
author_sort	Teresa Faddetta
title	The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
title_short	The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
title_full	The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
title_fullStr	The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
title_full_unstemmed	The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
title_sort	endophytic microbiota of citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55473990ceb741aba8f4828584f3bef8
work_keys_str_mv	AT teresafaddetta theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT loredanaabbate theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT pasqualealibrandi theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT walterarancio theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT davidesiino theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescostrati theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT carlottadefilippo theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT sergiofattadelbosco theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescocarimi theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT annamariapuglia theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT massimilianocardinale theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT giuseppegallo theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescomercati theendophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT teresafaddetta endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT loredanaabbate endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT pasqualealibrandi endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT walterarancio endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT davidesiino endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescostrati endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT carlottadefilippo endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT sergiofattadelbosco endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescocarimi endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT annamariapuglia endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT massimilianocardinale endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT giuseppegallo endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures AT francescomercati endophyticmicrobiotaofcitruslimonistransmittedfromseedtoshoothighlightingdifferencesofbacterialandfungalcommunitystructures
_version_	1718378273857077248

The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures

Ejemplares similares