AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells
Abstract The study of primary glucagon-secreting α-cells is hampered by their low abundance and scattered distribution in rodent pancreatic islets. We have designed a double-stranded adeno-associated virus containing a rat proglucagon promoter (700 bp) driving enhanced green fluorescent protein (AAV...
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Nature Portfolio
2019
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oai:doaj.org-article:556a5308d1314702b2cd07d70fc684432021-12-02T16:08:05ZAAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells10.1038/s41598-019-46735-22045-2322https://doaj.org/article/556a5308d1314702b2cd07d70fc684432019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46735-2https://doaj.org/toc/2045-2322Abstract The study of primary glucagon-secreting α-cells is hampered by their low abundance and scattered distribution in rodent pancreatic islets. We have designed a double-stranded adeno-associated virus containing a rat proglucagon promoter (700 bp) driving enhanced green fluorescent protein (AAV GCG-EGFP), to specifically identify α-cells. The administration of AAV GCG-EGFP by intraperitoneal or intraductal injection led to EGFP expression selectively in the α-cell population. AAV GCG-EGFP delivery to mice followed by islet isolation, dispersion and separation by FACS for EGFP resulted in an 86% pure population of α-cells. Furthermore, the administration of AAV GCG-EGFP at various doses to adult wild type mice did not significantly alter body weight, blood glucose, plasma insulin or glucagon levels, glucose tolerance or arginine tolerance. In vitro experiments in transgene positive α-cells demonstrated that EGFP expression did not alter the intracellular Ca2+ pattern in response to glucose or adrenaline. This approach may be useful for studying purified primary α-cells and for the in vivo delivery of other genes selectively to α-cells to further probe their function or to manipulate them for therapeutic purposes.Eva TuduríMaria M. GlavasAli AsadiRobert K. BakerCara E. EllisGalina SoukhatchevaMarjolaine PhilitFrank K. HuynhJames D. JohnsonC. Bruce VerchereTimothy J. KiefferNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
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Medicine R Science Q Eva Tudurí Maria M. Glavas Ali Asadi Robert K. Baker Cara E. Ellis Galina Soukhatcheva Marjolaine Philit Frank K. Huynh James D. Johnson C. Bruce Verchere Timothy J. Kieffer AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| description |
Abstract The study of primary glucagon-secreting α-cells is hampered by their low abundance and scattered distribution in rodent pancreatic islets. We have designed a double-stranded adeno-associated virus containing a rat proglucagon promoter (700 bp) driving enhanced green fluorescent protein (AAV GCG-EGFP), to specifically identify α-cells. The administration of AAV GCG-EGFP by intraperitoneal or intraductal injection led to EGFP expression selectively in the α-cell population. AAV GCG-EGFP delivery to mice followed by islet isolation, dispersion and separation by FACS for EGFP resulted in an 86% pure population of α-cells. Furthermore, the administration of AAV GCG-EGFP at various doses to adult wild type mice did not significantly alter body weight, blood glucose, plasma insulin or glucagon levels, glucose tolerance or arginine tolerance. In vitro experiments in transgene positive α-cells demonstrated that EGFP expression did not alter the intracellular Ca2+ pattern in response to glucose or adrenaline. This approach may be useful for studying purified primary α-cells and for the in vivo delivery of other genes selectively to α-cells to further probe their function or to manipulate them for therapeutic purposes. |
| format |
article |
| author |
Eva Tudurí Maria M. Glavas Ali Asadi Robert K. Baker Cara E. Ellis Galina Soukhatcheva Marjolaine Philit Frank K. Huynh James D. Johnson C. Bruce Verchere Timothy J. Kieffer |
| author_facet |
Eva Tudurí Maria M. Glavas Ali Asadi Robert K. Baker Cara E. Ellis Galina Soukhatcheva Marjolaine Philit Frank K. Huynh James D. Johnson C. Bruce Verchere Timothy J. Kieffer |
| author_sort |
Eva Tudurí |
| title |
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| title_short |
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| title_full |
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| title_fullStr |
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| title_full_unstemmed |
AAV GCG-EGFP, a new tool to identify glucagon-secreting α-cells |
| title_sort |
aav gcg-egfp, a new tool to identify glucagon-secreting α-cells |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/556a5308d1314702b2cd07d70fc68443 |
| work_keys_str_mv |
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