Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.

Lung diseases, including asthma, COPD, and other autoimmune lung pathologies are aggravated by exposure to particulate matter (PM) found in air pollution. IL-17 has been shown to exacerbate airway disease in animal models. As PM is known to contain aryl hydrocarbon receptor (AHR) ligands and the AHR...

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Autores principales:	Michael van Voorhis, Samantha Knopp, Walker Julliard, John H Fechner, Xiaoji Zhang, James J Schauer, Joshua D Mezrich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/556df3c4ab0341c0ba9b14124a97c29a
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spelling	oai:doaj.org-article:556df3c4ab0341c0ba9b14124a97c29a2021-11-18T08:42:25ZExposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.1932-620310.1371/journal.pone.0082545https://doaj.org/article/556df3c4ab0341c0ba9b14124a97c29a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349309/?tool=EBIhttps://doaj.org/toc/1932-6203Lung diseases, including asthma, COPD, and other autoimmune lung pathologies are aggravated by exposure to particulate matter (PM) found in air pollution. IL-17 has been shown to exacerbate airway disease in animal models. As PM is known to contain aryl hydrocarbon receptor (AHR) ligands and the AHR has recently been shown to play a role in differentiation of Th17 T cells, the aim of this study was to determine whether exposure to PM could impact Th17 polarization in an AHR-dependent manner. This study used both cell culture techniques and in vivo exposure in mice to examine the response of T cells to PM. Initially experiments were conducted with urban dust particles from a standard reference material, and ultimately repeated with freshly collected samples of diesel exhaust and cigarette smoke. The readout for the assays was increased T cell differentiation as indicated by increased generation of IL-17A in culture, and increased populations of IL-17 producing cells by intracellular flow cytometry. The data illustrate that Th17 polarization was significantly enhanced by addition of urban dust in a dose dependent fashion in cultures of wild-type but not AHR(-/-) mice. The data further suggest that polycyclic aromatic hydrocarbons played a primary role in this enhancement. There was both an increase of Th17 cell differentiation, and also an increase in the amount of IL-17 secreted by the cells. In summary, this paper identifies a novel mechanism whereby PM can directly act on the AHR in T cells, leading to enhanced Th17 differentiation. Further understanding of the molecular mechanisms responsible for pathologic Th17 differentiation and autoimmunity seen after exposure to pollution will allow direct targeting of proteins involved in AHR activation and function for treatment of PM exposures.Michael van VoorhisSamantha KnoppWalker JulliardJohn H FechnerXiaoji ZhangJames J SchauerJoshua D MezrichPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82545 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael van Voorhis Samantha Knopp Walker Julliard John H Fechner Xiaoji Zhang James J Schauer Joshua D Mezrich Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
description	Lung diseases, including asthma, COPD, and other autoimmune lung pathologies are aggravated by exposure to particulate matter (PM) found in air pollution. IL-17 has been shown to exacerbate airway disease in animal models. As PM is known to contain aryl hydrocarbon receptor (AHR) ligands and the AHR has recently been shown to play a role in differentiation of Th17 T cells, the aim of this study was to determine whether exposure to PM could impact Th17 polarization in an AHR-dependent manner. This study used both cell culture techniques and in vivo exposure in mice to examine the response of T cells to PM. Initially experiments were conducted with urban dust particles from a standard reference material, and ultimately repeated with freshly collected samples of diesel exhaust and cigarette smoke. The readout for the assays was increased T cell differentiation as indicated by increased generation of IL-17A in culture, and increased populations of IL-17 producing cells by intracellular flow cytometry. The data illustrate that Th17 polarization was significantly enhanced by addition of urban dust in a dose dependent fashion in cultures of wild-type but not AHR(-/-) mice. The data further suggest that polycyclic aromatic hydrocarbons played a primary role in this enhancement. There was both an increase of Th17 cell differentiation, and also an increase in the amount of IL-17 secreted by the cells. In summary, this paper identifies a novel mechanism whereby PM can directly act on the AHR in T cells, leading to enhanced Th17 differentiation. Further understanding of the molecular mechanisms responsible for pathologic Th17 differentiation and autoimmunity seen after exposure to pollution will allow direct targeting of proteins involved in AHR activation and function for treatment of PM exposures.
format	article
author	Michael van Voorhis Samantha Knopp Walker Julliard John H Fechner Xiaoji Zhang James J Schauer Joshua D Mezrich
author_facet	Michael van Voorhis Samantha Knopp Walker Julliard John H Fechner Xiaoji Zhang James J Schauer Joshua D Mezrich
author_sort	Michael van Voorhis
title	Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
title_short	Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
title_full	Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
title_fullStr	Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
title_full_unstemmed	Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.
title_sort	exposure to atmospheric particulate matter enhances th17 polarization through the aryl hydrocarbon receptor.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/556df3c4ab0341c0ba9b14124a97c29a
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Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.

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