Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways

Introduction Oxidative stress is currently proposed as a risk factor associated with the development and progression of osteoporosis. Here, the effect of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glycoside (THSG) on oxidative damage was investigated in an osteoblast-like MC3T3-E1 cell model. Material a...

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Autores principales:	Jian Cheng, Haohao Wang, Zhida Zhang, Keyong Liang
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2018
Materias:	nf-kb osteoblast oxidative damage thsg nf-κb nrf2/ho-1 Medicine R
Acceso en línea:	https://doaj.org/article/55765f96b3b947a8bfd67ef965353dc0
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spelling	oai:doaj.org-article:55765f96b3b947a8bfd67ef965353dc02021-12-02T18:39:09ZStilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways1734-19221896-915110.5114/aoms.2018.79937https://doaj.org/article/55765f96b3b947a8bfd67ef965353dc02018-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Stilbene-glycoside-protects-osteoblasts-against-oxidative-damage-via-Nrf2-HO-1-and,80618,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Oxidative stress is currently proposed as a risk factor associated with the development and progression of osteoporosis. Here, the effect of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glycoside (THSG) on oxidative damage was investigated in an osteoblast-like MC3T3-E1 cell model. Material and methods In this study, MC3T3-E1 cells were treated with hydrogen peroxide (H 2 O 2 ) (100 µM) and THSG (20, 50 and 100 µM), and alkaline phosphatase (ALP). ROS and MDA levels were measured using specific kits. Meanwhile, cell viability and apoptosis were also assessed using MTT methods and flow cytometry, respectively. Then, expression levels of Nrf2 and its downstream targets were determined using real-time PCR and western blotting, as well as the apoptosis related factors, including Bax, Bcl-2, caspase-3, and caspase-9. Results Upon H 2 O 2 treatment, cell viability was significantly decreased, while THSG clearly attenuated this decrease in a dose-dependent manner. Compared with the negative control, H 2 O 2 significantly decreased ALP and increased the levels of MDA, ROS and apoptosis, while THSG markedly reversed these effects in a dose-dependent manner. Moreover, THSG was identified to reverse the elevation of caspase-3, caspase-9 and Bax and the reduction of Bcl-2 induced by H 2 O 2 . For the Nrf2 signaling pathway, THSG was also observed to attenuate the up-regulation of Nrf2, HO-1, and NQO1, and the down-regulation of NF-κB induced by H 2 O 2 . Conclusions THSG could significantly attenuate oxidative damage induced by H 2 O 2 via the Nrf2/NF-κB signaling pathway, providing new insights for treatments of osteoporosis induced by oxidative injury.Jian ChengHaohao WangZhida ZhangKeyong LiangTermedia Publishing Housearticlenf-kbosteoblastoxidative damagethsgnf-κbnrf2/ho-1MedicineRENArchives of Medical Science, Vol 15, Iss 1, Pp 196-203 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nf-kb osteoblast oxidative damage thsg nf-κb nrf2/ho-1 Medicine R
spellingShingle	nf-kb osteoblast oxidative damage thsg nf-κb nrf2/ho-1 Medicine R Jian Cheng Haohao Wang Zhida Zhang Keyong Liang Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
description	Introduction Oxidative stress is currently proposed as a risk factor associated with the development and progression of osteoporosis. Here, the effect of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glycoside (THSG) on oxidative damage was investigated in an osteoblast-like MC3T3-E1 cell model. Material and methods In this study, MC3T3-E1 cells were treated with hydrogen peroxide (H 2 O 2 ) (100 µM) and THSG (20, 50 and 100 µM), and alkaline phosphatase (ALP). ROS and MDA levels were measured using specific kits. Meanwhile, cell viability and apoptosis were also assessed using MTT methods and flow cytometry, respectively. Then, expression levels of Nrf2 and its downstream targets were determined using real-time PCR and western blotting, as well as the apoptosis related factors, including Bax, Bcl-2, caspase-3, and caspase-9. Results Upon H 2 O 2 treatment, cell viability was significantly decreased, while THSG clearly attenuated this decrease in a dose-dependent manner. Compared with the negative control, H 2 O 2 significantly decreased ALP and increased the levels of MDA, ROS and apoptosis, while THSG markedly reversed these effects in a dose-dependent manner. Moreover, THSG was identified to reverse the elevation of caspase-3, caspase-9 and Bax and the reduction of Bcl-2 induced by H 2 O 2 . For the Nrf2 signaling pathway, THSG was also observed to attenuate the up-regulation of Nrf2, HO-1, and NQO1, and the down-regulation of NF-κB induced by H 2 O 2 . Conclusions THSG could significantly attenuate oxidative damage induced by H 2 O 2 via the Nrf2/NF-κB signaling pathway, providing new insights for treatments of osteoporosis induced by oxidative injury.
format	article
author	Jian Cheng Haohao Wang Zhida Zhang Keyong Liang
author_facet	Jian Cheng Haohao Wang Zhida Zhang Keyong Liang
author_sort	Jian Cheng
title	Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
title_short	Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
title_full	Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
title_fullStr	Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
title_full_unstemmed	Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways
title_sort	stilbene glycoside protects osteoblasts against oxidative damage via nrf2/ho-1 and nf-κb signaling pathways
publisher	Termedia Publishing House
publishDate	2018
url	https://doaj.org/article/55765f96b3b947a8bfd67ef965353dc0
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Stilbene glycoside protects osteoblasts against oxidative damage via Nrf2/HO-1 and NF-κB signaling pathways

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