Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).

Malaria sporozoites must invade the salivary glands of mosquitoes for maturation before transmission to vertebrate hosts. The duration of the sporogonic cycle within the mosquitoes ranges from 10 to 21 days depending on the parasite species and temperature. During blood feeding salivary gland protei...

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Autores principales:	Sriwatapron Sor-suwan, Narissara Jariyapan, Sittiruk Roytrakul, Atchara Paemanee, Atchara Phumee, Benjarat Phattanawiboon, Nuchpicha Intakhan, Wetpisit Chanmol, Paul A Bates, Atiporn Saeung, Wej Choochote
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55771d8db0804fc5a08efcbf6c82de9e
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spelling	oai:doaj.org-article:55771d8db0804fc5a08efcbf6c82de9e2021-11-18T08:29:32ZIdentification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).1932-620310.1371/journal.pone.0090809https://doaj.org/article/55771d8db0804fc5a08efcbf6c82de9e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24599352/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Malaria sporozoites must invade the salivary glands of mosquitoes for maturation before transmission to vertebrate hosts. The duration of the sporogonic cycle within the mosquitoes ranges from 10 to 21 days depending on the parasite species and temperature. During blood feeding salivary gland proteins are injected into the vertebrate host, along with malaria sporozoites in the case of an infected mosquito. To identify salivary gland proteins depleted after blood feeding of female Anopheles campestris-like, a potential malaria vector of Plasmodium vivax in Thailand, two-dimensional gel electrophoresis and nano-liquid chromatography-mass spectrometry techniques were used. Results showed that 19 major proteins were significantly depleted in three to four day-old mosquitoes fed on a first blood meal. For the mosquitoes fed the second blood meal on day 14 after the first blood meal, 14 major proteins were significantly decreased in amount. The significantly depleted proteins in both groups included apyrase, 5'-nucleotidase/apyrase, D7, D7-related 1, short form D7r1, gSG6, anti-platelet protein, serine/threonine-protein kinase rio3, putative sil1, cyclophilin A, hypothetical protein Phum_PHUM512530, AGAP007618-PA, and two non-significant hit proteins. To our knowledge, this study presents for the first time the salivary gland proteins that are involved in the second blood feeding on the day corresponding to the transmission period of the sporozoites to new mammalian hosts. This information serves as a basis for future work concerning the possible role of these proteins in the parasite transmission and the physiological processes that occur during the blood feeding.Sriwatapron Sor-suwanNarissara JariyapanSittiruk RoytrakulAtchara PaemaneeAtchara PhumeeBenjarat PhattanawiboonNuchpicha IntakhanWetpisit ChanmolPaul A BatesAtiporn SaeungWej ChoochotePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90809 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sriwatapron Sor-suwan Narissara Jariyapan Sittiruk Roytrakul Atchara Paemanee Atchara Phumee Benjarat Phattanawiboon Nuchpicha Intakhan Wetpisit Chanmol Paul A Bates Atiporn Saeung Wej Choochote Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
description	Malaria sporozoites must invade the salivary glands of mosquitoes for maturation before transmission to vertebrate hosts. The duration of the sporogonic cycle within the mosquitoes ranges from 10 to 21 days depending on the parasite species and temperature. During blood feeding salivary gland proteins are injected into the vertebrate host, along with malaria sporozoites in the case of an infected mosquito. To identify salivary gland proteins depleted after blood feeding of female Anopheles campestris-like, a potential malaria vector of Plasmodium vivax in Thailand, two-dimensional gel electrophoresis and nano-liquid chromatography-mass spectrometry techniques were used. Results showed that 19 major proteins were significantly depleted in three to four day-old mosquitoes fed on a first blood meal. For the mosquitoes fed the second blood meal on day 14 after the first blood meal, 14 major proteins were significantly decreased in amount. The significantly depleted proteins in both groups included apyrase, 5'-nucleotidase/apyrase, D7, D7-related 1, short form D7r1, gSG6, anti-platelet protein, serine/threonine-protein kinase rio3, putative sil1, cyclophilin A, hypothetical protein Phum_PHUM512530, AGAP007618-PA, and two non-significant hit proteins. To our knowledge, this study presents for the first time the salivary gland proteins that are involved in the second blood feeding on the day corresponding to the transmission period of the sporozoites to new mammalian hosts. This information serves as a basis for future work concerning the possible role of these proteins in the parasite transmission and the physiological processes that occur during the blood feeding.
format	article
author	Sriwatapron Sor-suwan Narissara Jariyapan Sittiruk Roytrakul Atchara Paemanee Atchara Phumee Benjarat Phattanawiboon Nuchpicha Intakhan Wetpisit Chanmol Paul A Bates Atiporn Saeung Wej Choochote
author_facet	Sriwatapron Sor-suwan Narissara Jariyapan Sittiruk Roytrakul Atchara Paemanee Atchara Phumee Benjarat Phattanawiboon Nuchpicha Intakhan Wetpisit Chanmol Paul A Bates Atiporn Saeung Wej Choochote
author_sort	Sriwatapron Sor-suwan
title	Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
title_short	Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
title_full	Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
title_fullStr	Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
title_full_unstemmed	Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).
title_sort	identification of salivary gland proteins depleted after blood feeding in the malaria vector anopheles campestris-like mosquitoes (diptera: culicidae).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/55771d8db0804fc5a08efcbf6c82de9e
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Identification of salivary gland proteins depleted after blood feeding in the malaria vector Anopheles campestris-like mosquitoes (Diptera: Culicidae).

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