A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>

Genome editing with CRISPR/Cas9 technology has advanced from the lab bench to clinical application with multiple trials underway. This article introduces a course-based undergraduate experience (CURE) combining CRISPR/Cas9 genome editing (using a modified two-plasmid system) and the animal model Cae...

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Autores principales:	Eric Hastie, Ryan Sellers, Bruno Valan, David R. Sherwood
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/557c2cec98fb4454a84c625d4850673c
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spelling	oai:doaj.org-article:557c2cec98fb4454a84c625d4850673c2021-11-15T15:04:12ZA Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>10.1128/jmbe.v20i3.18471935-78851935-7877https://doaj.org/article/557c2cec98fb4454a84c625d4850673c2019-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v20i3.1847https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Genome editing with CRISPR/Cas9 technology has advanced from the lab bench to clinical application with multiple trials underway. This article introduces a course-based undergraduate experience (CURE) combining CRISPR/Cas9 genome editing (using a modified two-plasmid system) and the animal model Caenorhabditis elegans. This CURE is designed to be a scalable, semester-long laboratory that will introduce the students to literature searches, molecular biology, experiment planning, microscopy, CRISPR bioethics discussion, and scientific writing. Here, students challenged themselves to endogenously tag the C. elegans gene zmp-4, a matrix metalloproteinase enzyme, with a fluorescent protein marker and successfully generated a new worm strain. The knock-in was confirmed with genotyping and imaging and will be available for use by the entire worm community.Eric HastieRyan SellersBruno ValanDavid R. SherwoodAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 20, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Eric Hastie Ryan Sellers Bruno Valan David R. Sherwood A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
description	Genome editing with CRISPR/Cas9 technology has advanced from the lab bench to clinical application with multiple trials underway. This article introduces a course-based undergraduate experience (CURE) combining CRISPR/Cas9 genome editing (using a modified two-plasmid system) and the animal model Caenorhabditis elegans. This CURE is designed to be a scalable, semester-long laboratory that will introduce the students to literature searches, molecular biology, experiment planning, microscopy, CRISPR bioethics discussion, and scientific writing. Here, students challenged themselves to endogenously tag the C. elegans gene zmp-4, a matrix metalloproteinase enzyme, with a fluorescent protein marker and successfully generated a new worm strain. The knock-in was confirmed with genotyping and imaging and will be available for use by the entire worm community.
format	article
author	Eric Hastie Ryan Sellers Bruno Valan David R. Sherwood
author_facet	Eric Hastie Ryan Sellers Bruno Valan David R. Sherwood
author_sort	Eric Hastie
title	A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
title_short	A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
title_full	A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
title_fullStr	A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
title_full_unstemmed	A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>
title_sort	scalable cure using a crispr/cas9 fluorescent protein knock-in strategy in <italic toggle="yes">caenorhabditis elegans</italic>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/557c2cec98fb4454a84c625d4850673c
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A Scalable CURE Using a CRISPR/Cas9 Fluorescent Protein Knock-In Strategy in <italic toggle="yes">Caenorhabditis elegans</italic>

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