Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations

Successful back-translating clinical biomarkers and molecular imaging methods of Alzheimer's disease (AD), including positron emission tomography (PET), are very valuable for the evaluation of new therapeutic strategies and increase the quality of preclinical studies. 18F-Fluorodeoxyglucose (FD...

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Autores principales:	Caroline Bouter, Caroline Irwin, Timon N. Franke, Nicola Beindorff, Yvonne Bouter
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	5XFAD Alzheimer model Alzheimer's disease positron - emission tomography 18F-Flourdesoxyglucose-PET 18F-Florbetaben-PET Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/55813cdb89144d8a9fe5d94d8bb4ad80
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spelling	oai:doaj.org-article:55813cdb89144d8a9fe5d94d8bb4ad802021-12-01T06:42:55ZQuantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations2296-858X10.3389/fmed.2021.745064https://doaj.org/article/55813cdb89144d8a9fe5d94d8bb4ad802021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.745064/fullhttps://doaj.org/toc/2296-858XSuccessful back-translating clinical biomarkers and molecular imaging methods of Alzheimer's disease (AD), including positron emission tomography (PET), are very valuable for the evaluation of new therapeutic strategies and increase the quality of preclinical studies. 18F-Fluorodeoxyglucose (FDG)–PET and 18F-Florbetaben–PET are clinically established biomarkers capturing two key pathological features of AD. However, the suitability of 18F-FDG– and amyloid–PET in the widely used 5XFAD mouse model of AD is still unclear. Furthermore, only data on male 5XFAD mice have been published so far, whereas studies in female mice and possible sex differences in 18F-FDG and 18F-Florbetaben uptake are missing. The aim of this study was to evaluate the suitability of 18F-FDG– and 18F-Florbetaben–PET in 7-month-old female 5XFAD and to assess possible sex differences between male and female 5XFAD mice. We could demonstrate that female 5XFAD mice showed a significant reduction in brain glucose metabolism and increased cerebral amyloid deposition compared with wild type animals, in accordance with the pathology seen in AD patients. Furthermore, we showed for the first time that the hypometabolism in 5XFAD mice is gender-dependent and more pronounced in female mice. Therefore, these results support the feasibility of small animal PET imaging with 18F-FDG- and 18F-Florbetaben in 5XFAD mice in both, male and female animals. Moreover, our findings highlight the need to account for sex differences in studies working with 5XFAD mice.Caroline BouterCaroline IrwinTimon N. FrankeNicola BeindorffYvonne BouterFrontiers Media S.A.article5XFAD Alzheimer modelAlzheimer's diseasepositron - emission tomography18F-Flourdesoxyglucose-PET18F-Florbetaben-PETMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	5XFAD Alzheimer model Alzheimer's disease positron - emission tomography 18F-Flourdesoxyglucose-PET 18F-Florbetaben-PET Medicine (General) R5-920
spellingShingle	5XFAD Alzheimer model Alzheimer's disease positron - emission tomography 18F-Flourdesoxyglucose-PET 18F-Florbetaben-PET Medicine (General) R5-920 Caroline Bouter Caroline Irwin Timon N. Franke Nicola Beindorff Yvonne Bouter Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
description	Successful back-translating clinical biomarkers and molecular imaging methods of Alzheimer's disease (AD), including positron emission tomography (PET), are very valuable for the evaluation of new therapeutic strategies and increase the quality of preclinical studies. 18F-Fluorodeoxyglucose (FDG)–PET and 18F-Florbetaben–PET are clinically established biomarkers capturing two key pathological features of AD. However, the suitability of 18F-FDG– and amyloid–PET in the widely used 5XFAD mouse model of AD is still unclear. Furthermore, only data on male 5XFAD mice have been published so far, whereas studies in female mice and possible sex differences in 18F-FDG and 18F-Florbetaben uptake are missing. The aim of this study was to evaluate the suitability of 18F-FDG– and 18F-Florbetaben–PET in 7-month-old female 5XFAD and to assess possible sex differences between male and female 5XFAD mice. We could demonstrate that female 5XFAD mice showed a significant reduction in brain glucose metabolism and increased cerebral amyloid deposition compared with wild type animals, in accordance with the pathology seen in AD patients. Furthermore, we showed for the first time that the hypometabolism in 5XFAD mice is gender-dependent and more pronounced in female mice. Therefore, these results support the feasibility of small animal PET imaging with 18F-FDG- and 18F-Florbetaben in 5XFAD mice in both, male and female animals. Moreover, our findings highlight the need to account for sex differences in studies working with 5XFAD mice.
format	article
author	Caroline Bouter Caroline Irwin Timon N. Franke Nicola Beindorff Yvonne Bouter
author_facet	Caroline Bouter Caroline Irwin Timon N. Franke Nicola Beindorff Yvonne Bouter
author_sort	Caroline Bouter
title	Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
title_short	Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
title_full	Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
title_fullStr	Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
title_full_unstemmed	Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations
title_sort	quantitative brain positron emission tomography in female 5xfad alzheimer mice: pathological features and sex-specific alterations
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/55813cdb89144d8a9fe5d94d8bb4ad80
work_keys_str_mv	AT carolinebouter quantitativebrainpositronemissiontomographyinfemale5xfadalzheimermicepathologicalfeaturesandsexspecificalterations AT carolineirwin quantitativebrainpositronemissiontomographyinfemale5xfadalzheimermicepathologicalfeaturesandsexspecificalterations AT timonnfranke quantitativebrainpositronemissiontomographyinfemale5xfadalzheimermicepathologicalfeaturesandsexspecificalterations AT nicolabeindorff quantitativebrainpositronemissiontomographyinfemale5xfadalzheimermicepathologicalfeaturesandsexspecificalterations AT yvonnebouter quantitativebrainpositronemissiontomographyinfemale5xfadalzheimermicepathologicalfeaturesandsexspecificalterations
_version_	1718405475010084864

Quantitative Brain Positron Emission Tomography in Female 5XFAD Alzheimer Mice: Pathological Features and Sex-Specific Alterations

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