Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens

Marine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects o...

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Autores principales:	Teketay Wassie, Zhuang Lu, Xinyi Duan, Chunyan Xie, Kefyalew Gebeyew, Zhang Yumei, Yulong Yin, Xin Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chicken Enteromorpha polysaccharide immunity intestinal integrity marine algae microbiota Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/558258e1abdd41e4814d58cea9420831
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spelling	oai:doaj.org-article:558258e1abdd41e4814d58cea94208312021-12-01T13:56:18ZDietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens2296-861X10.3389/fnut.2021.783819https://doaj.org/article/558258e1abdd41e4814d58cea94208312021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.783819/fullhttps://doaj.org/toc/2296-861XMarine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects of marine algae Enteromorpha (Ulva) prolifera polysaccharide (EP) supplementation on growth performance, immune response, and caecal microbiota of broiler chickens. A total of 200 1-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with ten replications of ten chickens in each replication. The dietary treatments consisted of the control group (fed basal diet), and EP group (received diet supplemented with 400 mg EP/kg diet). Results showed that chickens fed EP exhibited significantly higher (P < 0.05) body weight and average daily gain than the chicken-fed basal diet. In addition, significantly longer villus height, shorter crypt depth, and higher villus height to crypt depth ratio were observed in the jejunal and ileal tissues of chickens fed EP. EP supplementation upregulated the mRNA expression of NF-κB, TLR4, MyD88, IL-2, IFN-α, and IL-1β in the ileal and jejunal tissues (P < 0.05). Besides, we observed significantly higher (P < 0.05) short-chain volatile fatty acids (SCFAs) levels in the caecal contents of the EP group than in the control group. Furthermore, 16S-rRNA analysis revealed that EP supplementation altered gut microbiota and caused an abundance shift at the phylum and genus level in broiler chicken. Interestingly, we observed an association between microbiota and SCFAs production. Overall, this study demonstrated that supplementation of diet with EP promotes growth performance, improves intestinal immune response and integrity, and modulates the caecal microbiota of broiler chickens. This study highlighted the application of marine algae polysaccharides as an antibiotic alternative for chickens. Furthermore, it provides insight to develop marine algae polysaccharide-based functional food and therapeutic agent.Teketay WassieZhuang LuXinyi DuanXinyi DuanChunyan XieKefyalew GebeyewZhang YumeiZhang YumeiYulong YinYulong YinXin WuXin WuFrontiers Media S.A.articlechickenEnteromorpha polysaccharideimmunityintestinal integritymarine algaemicrobiotaNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken Enteromorpha polysaccharide immunity intestinal integrity marine algae microbiota Nutrition. Foods and food supply TX341-641
spellingShingle	chicken Enteromorpha polysaccharide immunity intestinal integrity marine algae microbiota Nutrition. Foods and food supply TX341-641 Teketay Wassie Zhuang Lu Xinyi Duan Xinyi Duan Chunyan Xie Kefyalew Gebeyew Zhang Yumei Zhang Yumei Yulong Yin Yulong Yin Xin Wu Xin Wu Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
description	Marine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects of marine algae Enteromorpha (Ulva) prolifera polysaccharide (EP) supplementation on growth performance, immune response, and caecal microbiota of broiler chickens. A total of 200 1-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with ten replications of ten chickens in each replication. The dietary treatments consisted of the control group (fed basal diet), and EP group (received diet supplemented with 400 mg EP/kg diet). Results showed that chickens fed EP exhibited significantly higher (P < 0.05) body weight and average daily gain than the chicken-fed basal diet. In addition, significantly longer villus height, shorter crypt depth, and higher villus height to crypt depth ratio were observed in the jejunal and ileal tissues of chickens fed EP. EP supplementation upregulated the mRNA expression of NF-κB, TLR4, MyD88, IL-2, IFN-α, and IL-1β in the ileal and jejunal tissues (P < 0.05). Besides, we observed significantly higher (P < 0.05) short-chain volatile fatty acids (SCFAs) levels in the caecal contents of the EP group than in the control group. Furthermore, 16S-rRNA analysis revealed that EP supplementation altered gut microbiota and caused an abundance shift at the phylum and genus level in broiler chicken. Interestingly, we observed an association between microbiota and SCFAs production. Overall, this study demonstrated that supplementation of diet with EP promotes growth performance, improves intestinal immune response and integrity, and modulates the caecal microbiota of broiler chickens. This study highlighted the application of marine algae polysaccharides as an antibiotic alternative for chickens. Furthermore, it provides insight to develop marine algae polysaccharide-based functional food and therapeutic agent.
format	article
author	Teketay Wassie Zhuang Lu Xinyi Duan Xinyi Duan Chunyan Xie Kefyalew Gebeyew Zhang Yumei Zhang Yumei Yulong Yin Yulong Yin Xin Wu Xin Wu
author_facet	Teketay Wassie Zhuang Lu Xinyi Duan Xinyi Duan Chunyan Xie Kefyalew Gebeyew Zhang Yumei Zhang Yumei Yulong Yin Yulong Yin Xin Wu Xin Wu
author_sort	Teketay Wassie
title	Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
title_short	Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
title_full	Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
title_fullStr	Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
title_full_unstemmed	Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens
title_sort	dietary enteromorpha polysaccharide enhances intestinal immune response, integrity, and caecal microbial activity of broiler chickens
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/558258e1abdd41e4814d58cea9420831
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Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens

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