Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice

Abstract All grass leaves are strap-shaped with a series of parallel veins running from base to tip, but the distance between each pair of veins, and the cell-types that develop between them, differs depending on whether the plant performs C3 or C4 photosynthesis. As part of a multinational effort t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peng Wang, Shanta Karki, Akshaya K. Biswal, Hsiang-Chun Lin, Mary Jacqueline Dionora, Govinda Rizal, Xiaojia Yin, Mara L. Schuler, Tom Hughes, Jim P. Fouracre, Basel Abu Jamous, Olga Sedelnikova, Shuen-Fang Lo, Anindya Bandyopadhyay, Su-May Yu, Steven Kelly, W. Paul Quick, Jane A. Langdale
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5585abd1373448a0abebcb740028b7fa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5585abd1373448a0abebcb740028b7fa
record_format	dspace
spelling	oai:doaj.org-article:5585abd1373448a0abebcb740028b7fa2021-12-02T11:51:12ZCandidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice10.1038/s41598-017-04361-w2045-2322https://doaj.org/article/5585abd1373448a0abebcb740028b7fa2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04361-whttps://doaj.org/toc/2045-2322Abstract All grass leaves are strap-shaped with a series of parallel veins running from base to tip, but the distance between each pair of veins, and the cell-types that develop between them, differs depending on whether the plant performs C3 or C4 photosynthesis. As part of a multinational effort to introduce C4 traits into rice to boost crop yield, candidate regulators of C4 leaf anatomy were previously identified through an analysis of maize leaf transcriptomes. Here we tested the potential of 60 of those candidate genes to alter leaf anatomy in rice. In each case, transgenic rice lines were generated in which the maize gene was constitutively expressed. Lines grouped into three phenotypic classes: (1) indistinguishable from wild-type; (2) aberrant shoot and/or root growth indicating possible perturbations to hormone homeostasis; and (3) altered secondary cell wall formation. One of the genes in class 3 defines a novel monocot-specific family. None of the genes were individually sufficient to induce C4-like vein patterning or cell-type differentiation in rice. A better understanding of gene function in C4 plants is now needed to inform more sophisticated engineering attempts to alter leaf anatomy in C3 plants.Peng WangShanta KarkiAkshaya K. BiswalHsiang-Chun LinMary Jacqueline DionoraGovinda RizalXiaojia YinMara L. SchulerTom HughesJim P. FouracreBasel Abu JamousOlga SedelnikovaShuen-Fang LoAnindya BandyopadhyaySu-May YuSteven KellyW. Paul QuickJane A. LangdaleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Wang Shanta Karki Akshaya K. Biswal Hsiang-Chun Lin Mary Jacqueline Dionora Govinda Rizal Xiaojia Yin Mara L. Schuler Tom Hughes Jim P. Fouracre Basel Abu Jamous Olga Sedelnikova Shuen-Fang Lo Anindya Bandyopadhyay Su-May Yu Steven Kelly W. Paul Quick Jane A. Langdale Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
description	Abstract All grass leaves are strap-shaped with a series of parallel veins running from base to tip, but the distance between each pair of veins, and the cell-types that develop between them, differs depending on whether the plant performs C3 or C4 photosynthesis. As part of a multinational effort to introduce C4 traits into rice to boost crop yield, candidate regulators of C4 leaf anatomy were previously identified through an analysis of maize leaf transcriptomes. Here we tested the potential of 60 of those candidate genes to alter leaf anatomy in rice. In each case, transgenic rice lines were generated in which the maize gene was constitutively expressed. Lines grouped into three phenotypic classes: (1) indistinguishable from wild-type; (2) aberrant shoot and/or root growth indicating possible perturbations to hormone homeostasis; and (3) altered secondary cell wall formation. One of the genes in class 3 defines a novel monocot-specific family. None of the genes were individually sufficient to induce C4-like vein patterning or cell-type differentiation in rice. A better understanding of gene function in C4 plants is now needed to inform more sophisticated engineering attempts to alter leaf anatomy in C3 plants.
format	article
author	Peng Wang Shanta Karki Akshaya K. Biswal Hsiang-Chun Lin Mary Jacqueline Dionora Govinda Rizal Xiaojia Yin Mara L. Schuler Tom Hughes Jim P. Fouracre Basel Abu Jamous Olga Sedelnikova Shuen-Fang Lo Anindya Bandyopadhyay Su-May Yu Steven Kelly W. Paul Quick Jane A. Langdale
author_facet	Peng Wang Shanta Karki Akshaya K. Biswal Hsiang-Chun Lin Mary Jacqueline Dionora Govinda Rizal Xiaojia Yin Mara L. Schuler Tom Hughes Jim P. Fouracre Basel Abu Jamous Olga Sedelnikova Shuen-Fang Lo Anindya Bandyopadhyay Su-May Yu Steven Kelly W. Paul Quick Jane A. Langdale
author_sort	Peng Wang
title	Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
title_short	Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
title_full	Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
title_fullStr	Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
title_full_unstemmed	Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice
title_sort	candidate regulators of early leaf development in maize perturb hormone signalling and secondary cell wall formation when constitutively expressed in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5585abd1373448a0abebcb740028b7fa
work_keys_str_mv	AT pengwang candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT shantakarki candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT akshayakbiswal candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT hsiangchunlin candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT maryjacquelinedionora candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT govindarizal candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT xiaojiayin candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT maralschuler candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT tomhughes candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT jimpfouracre candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT baselabujamous candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT olgasedelnikova candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT shuenfanglo candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT anindyabandyopadhyay candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT sumayyu candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT stevenkelly candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT wpaulquick candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice AT janealangdale candidateregulatorsofearlyleafdevelopmentinmaizeperturbhormonesignallingandsecondarycellwallformationwhenconstitutivelyexpressedinrice
_version_	1718395163326283776

Candidate regulators of Early Leaf Development in Maize Perturb Hormone Signalling and Secondary Cell Wall Formation When Constitutively Expressed in Rice

Ejemplares similares