Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues

Abstract Identifying molecular alterations occurring during cancer progression is essential for a deeper understanding of the underlying biological processes. Here we have analyzed cancerous and healthy prostate biopsies using nanoLC-MS(MS) to detect proteins with altered expression and N-glycosylat...

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Autores principales:	Simon Sugár, Gábor Tóth, Fanni Bugyi, Károly Vékey, Katalin Karászi, László Drahos, Lilla Turiák
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/558734aa26ea49b1b1b8c749c90177ec
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spelling	oai:doaj.org-article:558734aa26ea49b1b1b8c749c90177ec2021-12-02T16:36:37ZAlterations in protein expression and site-specific N-glycosylation of prostate cancer tissues10.1038/s41598-021-95417-52045-2322https://doaj.org/article/558734aa26ea49b1b1b8c749c90177ec2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95417-5https://doaj.org/toc/2045-2322Abstract Identifying molecular alterations occurring during cancer progression is essential for a deeper understanding of the underlying biological processes. Here we have analyzed cancerous and healthy prostate biopsies using nanoLC-MS(MS) to detect proteins with altered expression and N-glycosylation. We have identified 75 proteins with significantly changing expression during disease progression. The biological processes involved were assigned based on protein–protein interaction networks. These include cellular component organization, metabolic and localization processes. Multiple glycoproteins were identified with aberrant glycosylation in prostate cancer, where differences in glycosite-specific sialylation, fucosylation, and galactosylation were the most substantial. Many of the glycoproteins with altered N-glycosylation were extracellular matrix constituents, and are heavily involved in the establishment of the tumor microenvironment.Simon SugárGábor TóthFanni BugyiKároly VékeyKatalin KarásziLászló DrahosLilla TuriákNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simon Sugár Gábor Tóth Fanni Bugyi Károly Vékey Katalin Karászi László Drahos Lilla Turiák Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
description	Abstract Identifying molecular alterations occurring during cancer progression is essential for a deeper understanding of the underlying biological processes. Here we have analyzed cancerous and healthy prostate biopsies using nanoLC-MS(MS) to detect proteins with altered expression and N-glycosylation. We have identified 75 proteins with significantly changing expression during disease progression. The biological processes involved were assigned based on protein–protein interaction networks. These include cellular component organization, metabolic and localization processes. Multiple glycoproteins were identified with aberrant glycosylation in prostate cancer, where differences in glycosite-specific sialylation, fucosylation, and galactosylation were the most substantial. Many of the glycoproteins with altered N-glycosylation were extracellular matrix constituents, and are heavily involved in the establishment of the tumor microenvironment.
format	article
author	Simon Sugár Gábor Tóth Fanni Bugyi Károly Vékey Katalin Karászi László Drahos Lilla Turiák
author_facet	Simon Sugár Gábor Tóth Fanni Bugyi Károly Vékey Katalin Karászi László Drahos Lilla Turiák
author_sort	Simon Sugár
title	Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
title_short	Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
title_full	Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
title_fullStr	Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
title_full_unstemmed	Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues
title_sort	alterations in protein expression and site-specific n-glycosylation of prostate cancer tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/558734aa26ea49b1b1b8c749c90177ec
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Alterations in protein expression and site-specific N-glycosylation of prostate cancer tissues

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