A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.

Little is known about the genetic diversity of Salmonella enterica serovar Typhi (S. Typhi) circulating in Latin America. It has been observed that typhoid fever is still endemic in this part of the world; however, a lack of standardized blood culture surveillance across Latin American makes estimat...

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Autores principales:	Paula Diaz Guevara, Mailis Maes, Duy Pham Thanh, Carolina Duarte, Edna Catering Rodriguez, Lucy Angeline Montaño, Thanh Ho Ngoc Dan, To Nguyen Thi Nguyen, Megan E Carey, Josefina Campos, Isabel Chinen, Enrique Perez, Stephen Baker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/5588817c75e441a3a42b838557c94952
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spelling	oai:doaj.org-article:5588817c75e441a3a42b838557c949522021-12-02T20:24:06ZA genomic snapshot of Salmonella enterica serovar Typhi in Colombia.1935-27271935-273510.1371/journal.pntd.0009755https://doaj.org/article/5588817c75e441a3a42b838557c949522021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009755https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Little is known about the genetic diversity of Salmonella enterica serovar Typhi (S. Typhi) circulating in Latin America. It has been observed that typhoid fever is still endemic in this part of the world; however, a lack of standardized blood culture surveillance across Latin American makes estimating the true disease burden problematic. The Colombian National Health Service established a surveillance system for tracking bacterial pathogens, including S. Typhi, in 2006. Here, we characterized 77 representative Colombian S. Typhi isolates collected between 1997 and 2018 using pulse field gel electrophoresis (PFGE; the accepted genotyping method in Latin America) and whole genome sequencing (WGS). We found that the main S. Typhi clades circulating in Colombia were clades 2.5 and 3.5. Notably, the sequenced S. Typhi isolates from Colombia were closely related in a global phylogeny. Consequently, these data suggest that these are endemic clades circulating in Colombia. We found that AMR in S. Typhi in Colombia was uncommon, with a small subset of organisms exhibiting mutations associated with reduced susceptibility to fluoroquinolones. This is the first time that S. Typhi isolated from Colombia have been characterized by WGS, and after comparing these data with those generated using PFGE, we conclude that PFGE is unsuitable for tracking S. Typhi clones and mapping transmission. The genetic diversity of pathogens such as S. Typhi is limited in Latin America and should be targeted for future surveillance studies incorporating WGS.Paula Diaz GuevaraMailis MaesDuy Pham ThanhCarolina DuarteEdna Catering RodriguezLucy Angeline MontañoThanh Ho Ngoc DanTo Nguyen Thi NguyenMegan E CareyJosefina CamposIsabel ChinenEnrique PerezStephen BakerPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009755 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paula Diaz Guevara Mailis Maes Duy Pham Thanh Carolina Duarte Edna Catering Rodriguez Lucy Angeline Montaño Thanh Ho Ngoc Dan To Nguyen Thi Nguyen Megan E Carey Josefina Campos Isabel Chinen Enrique Perez Stephen Baker A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
description	Little is known about the genetic diversity of Salmonella enterica serovar Typhi (S. Typhi) circulating in Latin America. It has been observed that typhoid fever is still endemic in this part of the world; however, a lack of standardized blood culture surveillance across Latin American makes estimating the true disease burden problematic. The Colombian National Health Service established a surveillance system for tracking bacterial pathogens, including S. Typhi, in 2006. Here, we characterized 77 representative Colombian S. Typhi isolates collected between 1997 and 2018 using pulse field gel electrophoresis (PFGE; the accepted genotyping method in Latin America) and whole genome sequencing (WGS). We found that the main S. Typhi clades circulating in Colombia were clades 2.5 and 3.5. Notably, the sequenced S. Typhi isolates from Colombia were closely related in a global phylogeny. Consequently, these data suggest that these are endemic clades circulating in Colombia. We found that AMR in S. Typhi in Colombia was uncommon, with a small subset of organisms exhibiting mutations associated with reduced susceptibility to fluoroquinolones. This is the first time that S. Typhi isolated from Colombia have been characterized by WGS, and after comparing these data with those generated using PFGE, we conclude that PFGE is unsuitable for tracking S. Typhi clones and mapping transmission. The genetic diversity of pathogens such as S. Typhi is limited in Latin America and should be targeted for future surveillance studies incorporating WGS.
format	article
author	Paula Diaz Guevara Mailis Maes Duy Pham Thanh Carolina Duarte Edna Catering Rodriguez Lucy Angeline Montaño Thanh Ho Ngoc Dan To Nguyen Thi Nguyen Megan E Carey Josefina Campos Isabel Chinen Enrique Perez Stephen Baker
author_facet	Paula Diaz Guevara Mailis Maes Duy Pham Thanh Carolina Duarte Edna Catering Rodriguez Lucy Angeline Montaño Thanh Ho Ngoc Dan To Nguyen Thi Nguyen Megan E Carey Josefina Campos Isabel Chinen Enrique Perez Stephen Baker
author_sort	Paula Diaz Guevara
title	A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
title_short	A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
title_full	A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
title_fullStr	A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
title_full_unstemmed	A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.
title_sort	genomic snapshot of salmonella enterica serovar typhi in colombia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5588817c75e441a3a42b838557c94952
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A genomic snapshot of Salmonella enterica serovar Typhi in Colombia.

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