Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation

Shuo Zhang,1 Jingtao Gao,1 Shibo Liu,1 Lu Yu,1 Wen Zhang,1 Yinming Liang,1,2 Hui Wang1,2 1Henan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of China; 2Henan Collaborative Innovation Ce...

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Autores principales:	Zhang S, Gao J, Liu S, Yu L, Zhang W, Liang Y, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	inflammation obesity bcl3 lipid metabolism srebp1 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/558a01fecb2d4e77b95e458039adc3d7
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spelling	oai:doaj.org-article:558a01fecb2d4e77b95e458039adc3d72021-12-02T15:31:02ZTranscription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation1178-7031https://doaj.org/article/558a01fecb2d4e77b95e458039adc3d72021-09-01T00:00:00Zhttps://www.dovepress.com/transcription-coactivator-bcl3-acts-as-a-potential-regulator-of-lipid--peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Shuo Zhang,1 Jingtao Gao,1 Shibo Liu,1 Lu Yu,1 Wen Zhang,1 Yinming Liang,1,2 Hui Wang1,2 1Henan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of China; 2Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of ChinaCorrespondence: Hui WangHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of ChinaTel/Fax +86 373 3831203Email wanghui@xxmu.edu.cnBackground and Purpose: Transcriptional coactivator B-cell lymphoma-3 (BCL3) is a member of the IκB family of NF-κB inhibitors and regulates the activity of the NF-κB pathway. However, the relationship between BCL3 and lipid metabolism remains unclear. The present study investigates the effects of BCL3 in immune and metabolism in obese mice.Animals and Methods: Construct Bcl3-KO mice through CRISPR/Cas9 technology. Obesity model was induced in Bcl3-KO mice by feeding a high-fat diet for 16 weeks, and some metabolic-related indicators were analysed.Results: The results showed that the KO mice gained significantly less body weight on a high fat diet without a change in food intake. There was significant improvement in hepatic steatosis and adipose tissue hypertrophy in KO mice. The expression of SREBP1 and its downstream fatty acid synthetase FAS and ACC were down-regulated in KO mice, and the inflammation in adipose tissue and liver was further reduced.Conclusion: These results suggest that BCL3 may be a novel factor in regulating lipid metabolism in the development of obesity.Keywords: inflammation, obesity, BCL3, lipid metabolism, SREBP1Zhang SGao JLiu SYu LZhang WLiang YWang HDove Medical Pressarticleinflammationobesitybcl3lipid metabolismsrebp1PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 4915-4926 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammation obesity bcl3 lipid metabolism srebp1 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	inflammation obesity bcl3 lipid metabolism srebp1 Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Zhang S Gao J Liu S Yu L Zhang W Liang Y Wang H Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
description	Shuo Zhang,1 Jingtao Gao,1 Shibo Liu,1 Lu Yu,1 Wen Zhang,1 Yinming Liang,1,2 Hui Wang1,2 1Henan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of China; 2Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of ChinaCorrespondence: Hui WangHenan Key Laboratory of Immunology and Targeted Drug, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan Province, 453003, People’s Republic of ChinaTel/Fax +86 373 3831203Email wanghui@xxmu.edu.cnBackground and Purpose: Transcriptional coactivator B-cell lymphoma-3 (BCL3) is a member of the IκB family of NF-κB inhibitors and regulates the activity of the NF-κB pathway. However, the relationship between BCL3 and lipid metabolism remains unclear. The present study investigates the effects of BCL3 in immune and metabolism in obese mice.Animals and Methods: Construct Bcl3-KO mice through CRISPR/Cas9 technology. Obesity model was induced in Bcl3-KO mice by feeding a high-fat diet for 16 weeks, and some metabolic-related indicators were analysed.Results: The results showed that the KO mice gained significantly less body weight on a high fat diet without a change in food intake. There was significant improvement in hepatic steatosis and adipose tissue hypertrophy in KO mice. The expression of SREBP1 and its downstream fatty acid synthetase FAS and ACC were down-regulated in KO mice, and the inflammation in adipose tissue and liver was further reduced.Conclusion: These results suggest that BCL3 may be a novel factor in regulating lipid metabolism in the development of obesity.Keywords: inflammation, obesity, BCL3, lipid metabolism, SREBP1
format	article
author	Zhang S Gao J Liu S Yu L Zhang W Liang Y Wang H
author_facet	Zhang S Gao J Liu S Yu L Zhang W Liang Y Wang H
author_sort	Zhang S
title	Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
title_short	Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
title_full	Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
title_fullStr	Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
title_full_unstemmed	Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation
title_sort	transcription coactivator bcl3 acts as a potential regulator of lipid metabolism through the effects on inflammation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/558a01fecb2d4e77b95e458039adc3d7
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Transcription Coactivator BCL3 Acts as a Potential Regulator of Lipid Metabolism Through the Effects on Inflammation

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