Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy

Abstract Pregnancy requires a delicate immune balance that nurtures the allogeneic fetus, while maintaining reactivity against pathogens. Despite increasing knowledge, data is lacking on the transition of pre-pregnancy endometrial lymphocytes to a pregnancy state. Here, we immunophenotyped lymphocyt...

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Autores principales:	Dorien Feyaerts, Marilen Benner, Bram van Cranenbroek, Olivier W. H. van der Heijden, Irma Joosten, Renate G. van der Molen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/558a3c4ce0f74727aedd0fbc78d29fd1
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spelling	oai:doaj.org-article:558a3c4ce0f74727aedd0fbc78d29fd12021-12-02T12:30:12ZHuman uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy10.1038/s41598-017-03191-02045-2322https://doaj.org/article/558a3c4ce0f74727aedd0fbc78d29fd12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03191-0https://doaj.org/toc/2045-2322Abstract Pregnancy requires a delicate immune balance that nurtures the allogeneic fetus, while maintaining reactivity against pathogens. Despite increasing knowledge, data is lacking on the transition of pre-pregnancy endometrial lymphocytes to a pregnancy state. Here, we immunophenotyped lymphocytes from endometrium (MMC), term decidua parietalis (DPMC), and PBMC for direct comparison. We found that the immune cell composition of MMC and DPMC clearly differ from each other, with less NK-cells, and more NKT-cells and T-cells in DPMC. An increased percentage of central memory and effector memory T-cells, and less naive T-cells in DPMC indicates that decidual T-cells are more experienced than endometrial T-cells. The increased percentage of CD4+CD25highCD127− Treg in DPMC, including differentiated Treg, is indicative of a more experienced and tolerogenic environment during pregnancy. The Th cell composition of both MMC and DPMC was different from PBMC, with a preference for Th1 over Th2 in the uterine environment. Between MMC and DPMC, percentages of Th cell subsets did not differ significantly. Our results suggest that already before pregnancy a tightly controlled Th1/Th2/Th17 balance is present. These findings create opportunities to further investigate the underlying immune mechanism of pregnancy complications using menstrual blood as a source for endometrial lymphocytes.Dorien FeyaertsMarilen BennerBram van CranenbroekOlivier W. H. van der HeijdenIrma JoostenRenate G. van der MolenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorien Feyaerts Marilen Benner Bram van Cranenbroek Olivier W. H. van der Heijden Irma Joosten Renate G. van der Molen Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
description	Abstract Pregnancy requires a delicate immune balance that nurtures the allogeneic fetus, while maintaining reactivity against pathogens. Despite increasing knowledge, data is lacking on the transition of pre-pregnancy endometrial lymphocytes to a pregnancy state. Here, we immunophenotyped lymphocytes from endometrium (MMC), term decidua parietalis (DPMC), and PBMC for direct comparison. We found that the immune cell composition of MMC and DPMC clearly differ from each other, with less NK-cells, and more NKT-cells and T-cells in DPMC. An increased percentage of central memory and effector memory T-cells, and less naive T-cells in DPMC indicates that decidual T-cells are more experienced than endometrial T-cells. The increased percentage of CD4+CD25highCD127− Treg in DPMC, including differentiated Treg, is indicative of a more experienced and tolerogenic environment during pregnancy. The Th cell composition of both MMC and DPMC was different from PBMC, with a preference for Th1 over Th2 in the uterine environment. Between MMC and DPMC, percentages of Th cell subsets did not differ significantly. Our results suggest that already before pregnancy a tightly controlled Th1/Th2/Th17 balance is present. These findings create opportunities to further investigate the underlying immune mechanism of pregnancy complications using menstrual blood as a source for endometrial lymphocytes.
format	article
author	Dorien Feyaerts Marilen Benner Bram van Cranenbroek Olivier W. H. van der Heijden Irma Joosten Renate G. van der Molen
author_facet	Dorien Feyaerts Marilen Benner Bram van Cranenbroek Olivier W. H. van der Heijden Irma Joosten Renate G. van der Molen
author_sort	Dorien Feyaerts
title	Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
title_short	Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
title_full	Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
title_fullStr	Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
title_full_unstemmed	Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
title_sort	human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/558a3c4ce0f74727aedd0fbc78d29fd1
work_keys_str_mv	AT dorienfeyaerts humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy AT marilenbenner humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy AT bramvancranenbroek humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy AT olivierwhvanderheijden humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy AT irmajoosten humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy AT renategvandermolen humanuterinelymphocytesacquireamoreexperiencedandtolerogenicphenotypeduringpregnancy
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Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy

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