Modeling leukocyte-leukocyte non-contact interactions in a lymph node.

The interaction among leukocytes is at the basis of the innate and adaptive immune-response and it is largely ascribed to direct cell-cell contacts. However, the exchange of a number of chemical stimuli (chemokines) allows also non-contact interaction during the immunological response. We want here...

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Autores principales:	Nicola Gritti, Michele Caccia, Laura Sironi, Maddalena Collini, Laura D'Alfonso, Francesca Granucci, Ivan Zanoni, Giuseppe Chirico
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/558a87c3f5a94e408c74ffd9068a7306
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id	oai:doaj.org-article:558a87c3f5a94e408c74ffd9068a7306
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spelling	oai:doaj.org-article:558a87c3f5a94e408c74ffd9068a73062021-11-18T08:49:18ZModeling leukocyte-leukocyte non-contact interactions in a lymph node.1932-620310.1371/journal.pone.0076756https://doaj.org/article/558a87c3f5a94e408c74ffd9068a73062013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204669/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The interaction among leukocytes is at the basis of the innate and adaptive immune-response and it is largely ascribed to direct cell-cell contacts. However, the exchange of a number of chemical stimuli (chemokines) allows also non-contact interaction during the immunological response. We want here to evaluate the extent of the effect of the non-contact interactions on the observed leukocyte-leukocyte kinematics and their interaction duration. To this aim we adopt a simplified mean field description inspired by the Keller-Segel chemotaxis model, of which we report an analytical solution suited for slowly varying sources of chemokines. Since our focus is on the non-contact interactions, leukocyte-leukocyte contact interactions are simulated only by means of a space dependent friction coefficient of the cells. The analytical solution of the Keller-Segel model is then taken as the basis of numerical simulations of interactions between leukocytes and their duration. The mean field interaction force that we derive has a time-space separable form and depends on the chemotaxis sensitivity parameter as well as on the chemokines diffusion coefficient and their degradation rate. All these parameters affect the distribution of the interaction durations. We draw a successful qualitative comparison between simulated data and sets of experimental data for DC-NK cells interaction duration and other kinematic parameters. Remarkably, the predicted percentage of the leukocyte-leukocyte interactions falls in the experimental range and depends (~25% increase) upon the chemotactic parameter indicating a non-negligible direct effect of the non-contact interaction on the leukocyte interactions.Nicola GrittiMichele CacciaLaura SironiMaddalena ColliniLaura D'AlfonsoFrancesca GranucciIvan ZanoniGiuseppe ChiricoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76756 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicola Gritti Michele Caccia Laura Sironi Maddalena Collini Laura D'Alfonso Francesca Granucci Ivan Zanoni Giuseppe Chirico Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
description	The interaction among leukocytes is at the basis of the innate and adaptive immune-response and it is largely ascribed to direct cell-cell contacts. However, the exchange of a number of chemical stimuli (chemokines) allows also non-contact interaction during the immunological response. We want here to evaluate the extent of the effect of the non-contact interactions on the observed leukocyte-leukocyte kinematics and their interaction duration. To this aim we adopt a simplified mean field description inspired by the Keller-Segel chemotaxis model, of which we report an analytical solution suited for slowly varying sources of chemokines. Since our focus is on the non-contact interactions, leukocyte-leukocyte contact interactions are simulated only by means of a space dependent friction coefficient of the cells. The analytical solution of the Keller-Segel model is then taken as the basis of numerical simulations of interactions between leukocytes and their duration. The mean field interaction force that we derive has a time-space separable form and depends on the chemotaxis sensitivity parameter as well as on the chemokines diffusion coefficient and their degradation rate. All these parameters affect the distribution of the interaction durations. We draw a successful qualitative comparison between simulated data and sets of experimental data for DC-NK cells interaction duration and other kinematic parameters. Remarkably, the predicted percentage of the leukocyte-leukocyte interactions falls in the experimental range and depends (~25% increase) upon the chemotactic parameter indicating a non-negligible direct effect of the non-contact interaction on the leukocyte interactions.
format	article
author	Nicola Gritti Michele Caccia Laura Sironi Maddalena Collini Laura D'Alfonso Francesca Granucci Ivan Zanoni Giuseppe Chirico
author_facet	Nicola Gritti Michele Caccia Laura Sironi Maddalena Collini Laura D'Alfonso Francesca Granucci Ivan Zanoni Giuseppe Chirico
author_sort	Nicola Gritti
title	Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
title_short	Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
title_full	Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
title_fullStr	Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
title_full_unstemmed	Modeling leukocyte-leukocyte non-contact interactions in a lymph node.
title_sort	modeling leukocyte-leukocyte non-contact interactions in a lymph node.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/558a87c3f5a94e408c74ffd9068a7306
work_keys_str_mv	AT nicolagritti modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT michelecaccia modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT laurasironi modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT maddalenacollini modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT lauradalfonso modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT francescagranucci modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT ivanzanoni modelingleukocyteleukocytenoncontactinteractionsinalymphnode AT giuseppechirico modelingleukocyteleukocytenoncontactinteractionsinalymphnode
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Modeling leukocyte-leukocyte non-contact interactions in a lymph node.

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