Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky
Abstract Serological assays intended for diagnosis, sero-epidemiologic assessment, and measurement of protective antibody titers upon infection or vaccination are essential for managing the SARS-CoV-2 pandemic. Serological assays measuring the antibody responses against SARS-CoV-2 antigens are readi...
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2021
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oai:doaj.org-article:558a952dac6541968370f49755eb50782021-12-02T18:33:47ZSerological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky10.1038/s41598-021-97423-z2045-2322https://doaj.org/article/558a952dac6541968370f49755eb50782021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97423-zhttps://doaj.org/toc/2045-2322Abstract Serological assays intended for diagnosis, sero-epidemiologic assessment, and measurement of protective antibody titers upon infection or vaccination are essential for managing the SARS-CoV-2 pandemic. Serological assays measuring the antibody responses against SARS-CoV-2 antigens are readily available. However, some lack appropriate characteristics to accurately measure SARS-CoV-2 antibodies titers and neutralization. We developed an Enzyme-linked Immunosorbent Assay (ELISA) methods for measuring IgG, IgA, and IgM responses to SARS-CoV-2, Spike (S), receptor binding domain (RBD), and nucleocapsid (N) proteins. Performance characteristics of sensitivity and specificity have been defined. ELISA results show positive correlation with microneutralization and Plaque Reduction Neutralization assays with infectious SARS-CoV-2. Our ELISA was used to screen healthcare workers in Louisville, KY during the first wave of the local pandemic in the months of May and July 2020. We found a seropositive rate of approximately 1.4% and 2.3%, respectively. Our analyses demonstrate a broad immune response among individuals and suggest some non-RBD specific S IgG and IgA antibodies neutralize SARS-CoV-2.Krystal T. HamorskyAdrienne M. Bushau-SprinkleKathleen KittermanJulia M. CormanJennifer DeMarcoRachel J. KeithAruni BhatnagarJoshua L. FuquaAmanda LasnikJoongho JohDonghoon ChungJon KleinJoseph FlynnMarti GardnerShirish BarveSmita S. GhareKenneth E. PalmerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Krystal T. Hamorsky Adrienne M. Bushau-Sprinkle Kathleen Kitterman Julia M. Corman Jennifer DeMarco Rachel J. Keith Aruni Bhatnagar Joshua L. Fuqua Amanda Lasnik Joongho Joh Donghoon Chung Jon Klein Joseph Flynn Marti Gardner Shirish Barve Smita S. Ghare Kenneth E. Palmer Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
description |
Abstract Serological assays intended for diagnosis, sero-epidemiologic assessment, and measurement of protective antibody titers upon infection or vaccination are essential for managing the SARS-CoV-2 pandemic. Serological assays measuring the antibody responses against SARS-CoV-2 antigens are readily available. However, some lack appropriate characteristics to accurately measure SARS-CoV-2 antibodies titers and neutralization. We developed an Enzyme-linked Immunosorbent Assay (ELISA) methods for measuring IgG, IgA, and IgM responses to SARS-CoV-2, Spike (S), receptor binding domain (RBD), and nucleocapsid (N) proteins. Performance characteristics of sensitivity and specificity have been defined. ELISA results show positive correlation with microneutralization and Plaque Reduction Neutralization assays with infectious SARS-CoV-2. Our ELISA was used to screen healthcare workers in Louisville, KY during the first wave of the local pandemic in the months of May and July 2020. We found a seropositive rate of approximately 1.4% and 2.3%, respectively. Our analyses demonstrate a broad immune response among individuals and suggest some non-RBD specific S IgG and IgA antibodies neutralize SARS-CoV-2. |
format |
article |
author |
Krystal T. Hamorsky Adrienne M. Bushau-Sprinkle Kathleen Kitterman Julia M. Corman Jennifer DeMarco Rachel J. Keith Aruni Bhatnagar Joshua L. Fuqua Amanda Lasnik Joongho Joh Donghoon Chung Jon Klein Joseph Flynn Marti Gardner Shirish Barve Smita S. Ghare Kenneth E. Palmer |
author_facet |
Krystal T. Hamorsky Adrienne M. Bushau-Sprinkle Kathleen Kitterman Julia M. Corman Jennifer DeMarco Rachel J. Keith Aruni Bhatnagar Joshua L. Fuqua Amanda Lasnik Joongho Joh Donghoon Chung Jon Klein Joseph Flynn Marti Gardner Shirish Barve Smita S. Ghare Kenneth E. Palmer |
author_sort |
Krystal T. Hamorsky |
title |
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
title_short |
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
title_full |
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
title_fullStr |
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
title_full_unstemmed |
Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky |
title_sort |
serological assessment of sars-cov-2 infection during the first wave of the pandemic in louisville kentucky |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/558a952dac6541968370f49755eb5078 |
work_keys_str_mv |
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