Radiofrequency therapy improves exercise capacity of mice with emphysema
Abstract Emphysema is a common phenotype of chronic obstructive pulmonary disease (COPD). Although resection of emphysematous tissue can improve lung mechanics, it is invasive and fraught with adverse effects. Meanwhile, radiofrequency (RF) treatment is an extracorporeal method that leads to tissue...
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2021
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oai:doaj.org-article:558ab3fdc7524a94b30981ea348c52c62021-12-02T18:01:40ZRadiofrequency therapy improves exercise capacity of mice with emphysema10.1038/s41598-021-99474-82045-2322https://doaj.org/article/558ab3fdc7524a94b30981ea348c52c62021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99474-8https://doaj.org/toc/2045-2322Abstract Emphysema is a common phenotype of chronic obstructive pulmonary disease (COPD). Although resection of emphysematous tissue can improve lung mechanics, it is invasive and fraught with adverse effects. Meanwhile, radiofrequency (RF) treatment is an extracorporeal method that leads to tissue destruction and remodeling, resulting in “volume reduction” and overall improvement in lung compliance of emphysematous lungs. Whether these changes lead to improved exercise tolerance is unknown. Here, we investigated the effectiveness of RF treatment to improve the exercise capacity of mice with emphysema. Fifty-two mice (7 weeks of age) were used in this experiment. A bilateral emphysema model was created by intratracheally instilling porcine pancreatic elastase (PPE) (1.5U/100 g body weight). RF treatment (0.5 W/ g body weight) was administered extracorporeally 14 days later and mice were sacrificed after another 21 days. The exercise capacity of mice was measured using a treadmill. Treadmill runs were performed just before PPE instillation (baseline), before RF treatment and before sacrifice. Following sacrifice, lung compliance and mean linear intercept (Lm) were measured and fibrosis was assessed using a modified Ashcroft score. There were 3 experimental groups: controls (instilled with saline, n = 12), emphysema (instilled with porcine pancreatic elastase, PPE, n = 11) and emphysema + treatment (instilled with PPE and given RF, n = 9). At endpoint, the maximum velocity of the emphysema + treatment group was significantly higher than that of the emphysema group, indicating improved exercise tolerance (86.29% of baseline vs 61.69% of baseline, p = 0.01). Histological analysis revealed a significant reduction in emphysema as denoted by Lm between the two groups (median 29.60 µm vs 35.68 µm, p = 0.03). The emphysema + treatment group also demonstrated a higher prevalence of lung fibrosis (≧Grade 3) compared with the emphysema group (11.7% vs 5.4%, p < 0.01). No severe adverse events from RF were observed. RF treatment improved the exercise capacity of mice with emphysema. These data highlight the therapeutic potential of RF treatment in improving the functional status of patients with COPD.Mai TsutsuiChung Yan CheungTakeyuki WadaJen-erh JawCheng Wei Tony YangPascal BernatchezZoe WhiteChen Xi YangEun Jeong Annie BaeLauren H. ChoiDan GelbartSamuel LichtensteinLindsay MachanEran ElizurKim WolffEvan GoodacreMarek LipnickiDenny WongDon D. SinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
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Medicine R Science Q Mai Tsutsui Chung Yan Cheung Takeyuki Wada Jen-erh Jaw Cheng Wei Tony Yang Pascal Bernatchez Zoe White Chen Xi Yang Eun Jeong Annie Bae Lauren H. Choi Dan Gelbart Samuel Lichtenstein Lindsay Machan Eran Elizur Kim Wolff Evan Goodacre Marek Lipnicki Denny Wong Don D. Sin Radiofrequency therapy improves exercise capacity of mice with emphysema |
| description |
Abstract Emphysema is a common phenotype of chronic obstructive pulmonary disease (COPD). Although resection of emphysematous tissue can improve lung mechanics, it is invasive and fraught with adverse effects. Meanwhile, radiofrequency (RF) treatment is an extracorporeal method that leads to tissue destruction and remodeling, resulting in “volume reduction” and overall improvement in lung compliance of emphysematous lungs. Whether these changes lead to improved exercise tolerance is unknown. Here, we investigated the effectiveness of RF treatment to improve the exercise capacity of mice with emphysema. Fifty-two mice (7 weeks of age) were used in this experiment. A bilateral emphysema model was created by intratracheally instilling porcine pancreatic elastase (PPE) (1.5U/100 g body weight). RF treatment (0.5 W/ g body weight) was administered extracorporeally 14 days later and mice were sacrificed after another 21 days. The exercise capacity of mice was measured using a treadmill. Treadmill runs were performed just before PPE instillation (baseline), before RF treatment and before sacrifice. Following sacrifice, lung compliance and mean linear intercept (Lm) were measured and fibrosis was assessed using a modified Ashcroft score. There were 3 experimental groups: controls (instilled with saline, n = 12), emphysema (instilled with porcine pancreatic elastase, PPE, n = 11) and emphysema + treatment (instilled with PPE and given RF, n = 9). At endpoint, the maximum velocity of the emphysema + treatment group was significantly higher than that of the emphysema group, indicating improved exercise tolerance (86.29% of baseline vs 61.69% of baseline, p = 0.01). Histological analysis revealed a significant reduction in emphysema as denoted by Lm between the two groups (median 29.60 µm vs 35.68 µm, p = 0.03). The emphysema + treatment group also demonstrated a higher prevalence of lung fibrosis (≧Grade 3) compared with the emphysema group (11.7% vs 5.4%, p < 0.01). No severe adverse events from RF were observed. RF treatment improved the exercise capacity of mice with emphysema. These data highlight the therapeutic potential of RF treatment in improving the functional status of patients with COPD. |
| format |
article |
| author |
Mai Tsutsui Chung Yan Cheung Takeyuki Wada Jen-erh Jaw Cheng Wei Tony Yang Pascal Bernatchez Zoe White Chen Xi Yang Eun Jeong Annie Bae Lauren H. Choi Dan Gelbart Samuel Lichtenstein Lindsay Machan Eran Elizur Kim Wolff Evan Goodacre Marek Lipnicki Denny Wong Don D. Sin |
| author_facet |
Mai Tsutsui Chung Yan Cheung Takeyuki Wada Jen-erh Jaw Cheng Wei Tony Yang Pascal Bernatchez Zoe White Chen Xi Yang Eun Jeong Annie Bae Lauren H. Choi Dan Gelbart Samuel Lichtenstein Lindsay Machan Eran Elizur Kim Wolff Evan Goodacre Marek Lipnicki Denny Wong Don D. Sin |
| author_sort |
Mai Tsutsui |
| title |
Radiofrequency therapy improves exercise capacity of mice with emphysema |
| title_short |
Radiofrequency therapy improves exercise capacity of mice with emphysema |
| title_full |
Radiofrequency therapy improves exercise capacity of mice with emphysema |
| title_fullStr |
Radiofrequency therapy improves exercise capacity of mice with emphysema |
| title_full_unstemmed |
Radiofrequency therapy improves exercise capacity of mice with emphysema |
| title_sort |
radiofrequency therapy improves exercise capacity of mice with emphysema |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/558ab3fdc7524a94b30981ea348c52c6 |
| work_keys_str_mv |
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