Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)

<i>Aspidosperma pyrifolium</i> is used in traditional medicine to treat inflammatory disorders. The aim of the study was to perform phytochemical characterization and evaluate the anti-inflammatory, anti-nociceptive and acute toxicity effects of the total alkaloid fraction (TAF-Ap) from...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francisca Sabrina Vieira Lins, Vanessa Farias da Silva, Josean Fechine Tavares, Vanda Lúcia dos Santos, Harley da Silva Alves
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pereiro-preto plumeran alkaloids acute toxicity anti-inflammatory anti-nociceptive Botany QK1-989
Acceso en línea:	https://doaj.org/article/558dc4403ba84614b19335b71e9d8f73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:558dc4403ba84614b19335b71e9d8f73
record_format	dspace
spelling	oai:doaj.org-article:558dc4403ba84614b19335b71e9d8f732021-11-25T18:47:24ZBioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)10.3390/plants101125262223-7747https://doaj.org/article/558dc4403ba84614b19335b71e9d8f732021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2526https://doaj.org/toc/2223-7747<i>Aspidosperma pyrifolium</i> is used in traditional medicine to treat inflammatory disorders. The aim of the study was to perform phytochemical characterization and evaluate the anti-inflammatory, anti-nociceptive and acute toxicity effects of the total alkaloid fraction (TAF-Ap) from stem barks. Two monoterpenic indole alkaloids were isolated by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and the structural elucidation was performed using 1D and 2D NMR analysis. As for toxicity, no animals died at 50 mg kg<sup>−1</sup> and this concentration presented mild sedation and forced breathing within the first 24 h. The lethal dose capable of killing 50% of the animals (LD<sub>50</sub>) was estimated to be 160 mg kg<sup>−1</sup>. In the pharmacological tests, the models used were 1% carrageenan-induced paw edema and peritonitis, 1% formalin-induced nociception and 1% acetic acid-induced abdominal writhing in Swiss mice. The study made it possible to isolate 15-methoxyaspidospermine and 15-methoxypyrifolidine, corroborating the results of pharmacological assays, which showed anti-inflammatory and analgesic potential, especially at 30 mg kg<sup>−1</sup> (<i>p</i> < 0.001). Thus, the species was shown to be a promising source of active substances, with special attention paid to its toxicological potential.Francisca Sabrina Vieira LinsVanessa Farias da SilvaJosean Fechine TavaresVanda Lúcia dos SantosHarley da Silva AlvesMDPI AGarticlepereiro-pretoplumeran alkaloidsacute toxicityanti-inflammatoryanti-nociceptiveBotanyQK1-989ENPlants, Vol 10, Iss 2526, p 2526 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pereiro-preto plumeran alkaloids acute toxicity anti-inflammatory anti-nociceptive Botany QK1-989
spellingShingle	pereiro-preto plumeran alkaloids acute toxicity anti-inflammatory anti-nociceptive Botany QK1-989 Francisca Sabrina Vieira Lins Vanessa Farias da Silva Josean Fechine Tavares Vanda Lúcia dos Santos Harley da Silva Alves Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
description	<i>Aspidosperma pyrifolium</i> is used in traditional medicine to treat inflammatory disorders. The aim of the study was to perform phytochemical characterization and evaluate the anti-inflammatory, anti-nociceptive and acute toxicity effects of the total alkaloid fraction (TAF-Ap) from stem barks. Two monoterpenic indole alkaloids were isolated by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and the structural elucidation was performed using 1D and 2D NMR analysis. As for toxicity, no animals died at 50 mg kg<sup>−1</sup> and this concentration presented mild sedation and forced breathing within the first 24 h. The lethal dose capable of killing 50% of the animals (LD<sub>50</sub>) was estimated to be 160 mg kg<sup>−1</sup>. In the pharmacological tests, the models used were 1% carrageenan-induced paw edema and peritonitis, 1% formalin-induced nociception and 1% acetic acid-induced abdominal writhing in Swiss mice. The study made it possible to isolate 15-methoxyaspidospermine and 15-methoxypyrifolidine, corroborating the results of pharmacological assays, which showed anti-inflammatory and analgesic potential, especially at 30 mg kg<sup>−1</sup> (<i>p</i> < 0.001). Thus, the species was shown to be a promising source of active substances, with special attention paid to its toxicological potential.
format	article
author	Francisca Sabrina Vieira Lins Vanessa Farias da Silva Josean Fechine Tavares Vanda Lúcia dos Santos Harley da Silva Alves
author_facet	Francisca Sabrina Vieira Lins Vanessa Farias da Silva Josean Fechine Tavares Vanda Lúcia dos Santos Harley da Silva Alves
author_sort	Francisca Sabrina Vieira Lins
title	Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
title_short	Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
title_full	Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
title_fullStr	Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
title_full_unstemmed	Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)
title_sort	bioguided isolation of alkaloids and pharmacological effects of the total alkaloid fraction from <i>aspidosperma pyrifolium</i> mart. (apocynaceae)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/558dc4403ba84614b19335b71e9d8f73
work_keys_str_mv	AT franciscasabrinavieiralins bioguidedisolationofalkaloidsandpharmacologicaleffectsofthetotalalkaloidfractionfromiaspidospermapyrifoliumimartapocynaceae AT vanessafariasdasilva bioguidedisolationofalkaloidsandpharmacologicaleffectsofthetotalalkaloidfractionfromiaspidospermapyrifoliumimartapocynaceae AT joseanfechinetavares bioguidedisolationofalkaloidsandpharmacologicaleffectsofthetotalalkaloidfractionfromiaspidospermapyrifoliumimartapocynaceae AT vandaluciadossantos bioguidedisolationofalkaloidsandpharmacologicaleffectsofthetotalalkaloidfractionfromiaspidospermapyrifoliumimartapocynaceae AT harleydasilvaalves bioguidedisolationofalkaloidsandpharmacologicaleffectsofthetotalalkaloidfractionfromiaspidospermapyrifoliumimartapocynaceae
_version_	1718410712421761024

Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from <i>Aspidosperma pyrifolium</i> Mart. (Apocynaceae)

Ejemplares similares