Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure

Abstract Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study reveal...

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Autores principales:	Li Su, Changli Yang, Jianyu Meng, Lv Zhou, Changyu Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/559134ca3d3b465fa4acb247851d9db9
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spelling	oai:doaj.org-article:559134ca3d3b465fa4acb247851d9db92021-12-02T16:36:12ZComparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure10.1038/s41598-021-86269-02045-2322https://doaj.org/article/559134ca3d3b465fa4acb247851d9db92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86269-0https://doaj.org/toc/2045-2322Abstract Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study revealed differentially expressed genes (DEGs) and differently expressed metabolites (DEMs) in O. furnacalis under UV-A exposure. Three complementary DNA libraries were constructed from O. furnacalis adult females (CK, UV1h, and UV2h), and 50,106 expressed genes were obtained through Illumina sequencing. Of these, 157 and 637 DEGs were detected in UV1h and UV2h after UV-A exposure for 1 and 2 h, respectively, compared to CK, with 103 and 444 upregulated and 54 and 193 downregulated genes, respectively. Forty four DEGs were detected in UV2h compared to UV1h. Comparative transcriptome analysis between UV-treated and control groups revealed signal transduction, detoxification and stress response, immune defense, and antioxidative system involvement. Metabolomics analysis showed that 181 (UV1h vs. CK), 111 (UV2h vs. CK), and 34 (UV2h vs. UV1h) DEMs were obtained in positive ion mode, while 135 (UV1h vs. CK), 93 (UV2h vs. CK), and 36 (UV2h vs. UV1h) DEMs were obtained in negative ion mode. Moreover, UV-A exposure disturbed amino acid, sugar, and lipid metabolism. These findings provide insight for further studies on how insects protect themselves under UV-A stress.Li SuChangli YangJianyu MengLv ZhouChangyu ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Su Changli Yang Jianyu Meng Lv Zhou Changyu Zhang Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
description	Abstract Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study revealed differentially expressed genes (DEGs) and differently expressed metabolites (DEMs) in O. furnacalis under UV-A exposure. Three complementary DNA libraries were constructed from O. furnacalis adult females (CK, UV1h, and UV2h), and 50,106 expressed genes were obtained through Illumina sequencing. Of these, 157 and 637 DEGs were detected in UV1h and UV2h after UV-A exposure for 1 and 2 h, respectively, compared to CK, with 103 and 444 upregulated and 54 and 193 downregulated genes, respectively. Forty four DEGs were detected in UV2h compared to UV1h. Comparative transcriptome analysis between UV-treated and control groups revealed signal transduction, detoxification and stress response, immune defense, and antioxidative system involvement. Metabolomics analysis showed that 181 (UV1h vs. CK), 111 (UV2h vs. CK), and 34 (UV2h vs. UV1h) DEMs were obtained in positive ion mode, while 135 (UV1h vs. CK), 93 (UV2h vs. CK), and 36 (UV2h vs. UV1h) DEMs were obtained in negative ion mode. Moreover, UV-A exposure disturbed amino acid, sugar, and lipid metabolism. These findings provide insight for further studies on how insects protect themselves under UV-A stress.
format	article
author	Li Su Changli Yang Jianyu Meng Lv Zhou Changyu Zhang
author_facet	Li Su Changli Yang Jianyu Meng Lv Zhou Changyu Zhang
author_sort	Li Su
title	Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
title_short	Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
title_full	Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
title_fullStr	Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
title_full_unstemmed	Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure
title_sort	comparative transcriptome and metabolome analysis of ostrinia furnacalis female adults under uv-a exposure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/559134ca3d3b465fa4acb247851d9db9
work_keys_str_mv	AT lisu comparativetranscriptomeandmetabolomeanalysisofostriniafurnacalisfemaleadultsunderuvaexposure AT changliyang comparativetranscriptomeandmetabolomeanalysisofostriniafurnacalisfemaleadultsunderuvaexposure AT jianyumeng comparativetranscriptomeandmetabolomeanalysisofostriniafurnacalisfemaleadultsunderuvaexposure AT lvzhou comparativetranscriptomeandmetabolomeanalysisofostriniafurnacalisfemaleadultsunderuvaexposure AT changyuzhang comparativetranscriptomeandmetabolomeanalysisofostriniafurnacalisfemaleadultsunderuvaexposure
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Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure

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