APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES

1222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory...

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Autores principales:	I. A. Pashnina, I. M. Krivolapova, I. A. Tuzankina, V. A. Chereshnev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2012
Materias:	autoantibodies autoimmune diseases Science Q
Acceso en línea:	https://doaj.org/article/559191a0e9af472a837725fbd40564a4
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spelling	oai:doaj.org-article:559191a0e9af472a837725fbd40564a42021-11-23T06:14:19ZAPPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES2541-94202587-9596https://doaj.org/article/559191a0e9af472a837725fbd40564a42012-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/905https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-95961222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory comparability of different laboratory methods. The most sensitive method for detection of antinuclear antibodies is indirect immunofluorescence. This is a preferable method for screening of autoimmune connective tissue diseases. At comparison of luminescence types in immunofluorescence test and results of immunoblotting was shown that for each type of a luminescence the set of antibody, revealed by immunoblotting, was characteristic. However, the same antibodies can be found in various types of fluorescence that complicates unequivocal interpretation of immunofluorescence test results. Antibodies to Ro-52 were most often found in all types of fluorescence, and also in the absence of that.I. A. PashninaI. M. KrivolapovaI. A. TuzankinaV. A. ChereshnevScientific Сentre for Family Health and Human Reproduction Problemsarticleautoantibodiesautoimmune diseasesScienceQRUActa Biomedica Scientifica, Vol 0, Iss 3(2), Pp 143-147 (2012)
institution	DOAJ
collection	DOAJ
language	RU
topic	autoantibodies autoimmune diseases Science Q
spellingShingle	autoantibodies autoimmune diseases Science Q I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
description	1222 patients with suspicion of different autoimmune connective tissue diseases are investigated. Antinuclear antibodies by indirect immunofluorescence reaction, by methods of ELISA and immunoblotting were determined. Laboratory tests results correlated with each other that testifies to satisfactory comparability of different laboratory methods. The most sensitive method for detection of antinuclear antibodies is indirect immunofluorescence. This is a preferable method for screening of autoimmune connective tissue diseases. At comparison of luminescence types in immunofluorescence test and results of immunoblotting was shown that for each type of a luminescence the set of antibody, revealed by immunoblotting, was characteristic. However, the same antibodies can be found in various types of fluorescence that complicates unequivocal interpretation of immunofluorescence test results. Antibodies to Ro-52 were most often found in all types of fluorescence, and also in the absence of that.
format	article
author	I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev
author_facet	I. A. Pashnina I. M. Krivolapova I. A. Tuzankina V. A. Chereshnev
author_sort	I. A. Pashnina
title	APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
title_short	APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
title_full	APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
title_fullStr	APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
title_full_unstemmed	APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES
title_sort	application of different laboratory methods for antinuclear autoantibodies investigation in patients with autoimmune connective tissue diseases
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2012
url	https://doaj.org/article/559191a0e9af472a837725fbd40564a4
work_keys_str_mv	AT iapashnina applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT imkrivolapova applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT iatuzankina applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases AT vachereshnev applicationofdifferentlaboratorymethodsforantinuclearautoantibodiesinvestigationinpatientswithautoimmuneconnectivetissuediseases
_version_	1718417317780520960

APPLICATION OF DIFFERENT LABORATORY METHODS FOR ANTINUCLEAR AUTOANTIBODIES INVESTIGATION IN PATIENTS WITH AUTOIMMUNE CONNECTIVE TISSUE DISEASES

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