Distinct roles of haspin in stem cell division and male gametogenesis

Abstract The kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex at the centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing...

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Autores principales:	Katerina Soupsana, Eleftheria Karanika, Fani Kiosse, Anastasia Christogianni, Yiorgos Sfikas, Pantelis Topalis, Anna Batistatou, Zoi Kanaki, Apostolos Klinakis, Anastasia S. Politou, Spyros Georgatos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55980d2f4450420aa4ca54db29566fe1
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spelling	oai:doaj.org-article:55980d2f4450420aa4ca54db29566fe12021-12-02T18:37:08ZDistinct roles of haspin in stem cell division and male gametogenesis10.1038/s41598-021-99307-82045-2322https://doaj.org/article/55980d2f4450420aa4ca54db29566fe12021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99307-8https://doaj.org/toc/2045-2322Abstract The kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex at the centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing cells, haspin-null mice do not exhibit developmental anomalies, apart from aberrant testis architecture. Investigating this problem, we show here that mouse embryonic stem cells that lack or overexpress haspin, albeit prone to chromosome misalignment during metaphase, can still divide, expand and differentiate. RNA sequencing reveals that haspin dosage affects severely the expression levels of several genes that are involved in male gametogenesis. Consistent with a role in testis-specific expression, H3T3ph is detected not only in mitotic spermatogonia and meiotic spermatocytes, but also in non-dividing cells, such as haploid spermatids. Similarly to somatic cells, the mark is erased in the end of meiotic divisions, but re-installed during spermatid maturation, subsequent to methylation of histone H3 at lysine-4 (H3K4me3) and arginine-8 (H3R8me2). These serial modifications are particularly enriched in chromatin domains containing histone H3 trimethylated at lysine-27 (H3K27me3), but devoid of histone H3 trimethylated at lysine-9 (H3K9me3). The unique spatio-temporal pattern of histone H3 modifications implicates haspin in the epigenetic control of spermiogenesis.Katerina SoupsanaEleftheria KaranikaFani KiosseAnastasia ChristogianniYiorgos SfikasPantelis TopalisAnna BatistatouZoi KanakiApostolos KlinakisAnastasia S. PolitouSpyros GeorgatosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katerina Soupsana Eleftheria Karanika Fani Kiosse Anastasia Christogianni Yiorgos Sfikas Pantelis Topalis Anna Batistatou Zoi Kanaki Apostolos Klinakis Anastasia S. Politou Spyros Georgatos Distinct roles of haspin in stem cell division and male gametogenesis
description	Abstract The kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex at the centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing cells, haspin-null mice do not exhibit developmental anomalies, apart from aberrant testis architecture. Investigating this problem, we show here that mouse embryonic stem cells that lack or overexpress haspin, albeit prone to chromosome misalignment during metaphase, can still divide, expand and differentiate. RNA sequencing reveals that haspin dosage affects severely the expression levels of several genes that are involved in male gametogenesis. Consistent with a role in testis-specific expression, H3T3ph is detected not only in mitotic spermatogonia and meiotic spermatocytes, but also in non-dividing cells, such as haploid spermatids. Similarly to somatic cells, the mark is erased in the end of meiotic divisions, but re-installed during spermatid maturation, subsequent to methylation of histone H3 at lysine-4 (H3K4me3) and arginine-8 (H3R8me2). These serial modifications are particularly enriched in chromatin domains containing histone H3 trimethylated at lysine-27 (H3K27me3), but devoid of histone H3 trimethylated at lysine-9 (H3K9me3). The unique spatio-temporal pattern of histone H3 modifications implicates haspin in the epigenetic control of spermiogenesis.
format	article
author	Katerina Soupsana Eleftheria Karanika Fani Kiosse Anastasia Christogianni Yiorgos Sfikas Pantelis Topalis Anna Batistatou Zoi Kanaki Apostolos Klinakis Anastasia S. Politou Spyros Georgatos
author_facet	Katerina Soupsana Eleftheria Karanika Fani Kiosse Anastasia Christogianni Yiorgos Sfikas Pantelis Topalis Anna Batistatou Zoi Kanaki Apostolos Klinakis Anastasia S. Politou Spyros Georgatos
author_sort	Katerina Soupsana
title	Distinct roles of haspin in stem cell division and male gametogenesis
title_short	Distinct roles of haspin in stem cell division and male gametogenesis
title_full	Distinct roles of haspin in stem cell division and male gametogenesis
title_fullStr	Distinct roles of haspin in stem cell division and male gametogenesis
title_full_unstemmed	Distinct roles of haspin in stem cell division and male gametogenesis
title_sort	distinct roles of haspin in stem cell division and male gametogenesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55980d2f4450420aa4ca54db29566fe1
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Distinct roles of haspin in stem cell division and male gametogenesis

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