Immobilization of active ammonia-oxidizing archaea in hydrogel beads

Abstract Ammonia-oxidizing archaea (AOA) are major players in the nitrogen cycle but their cultivation represents a major challenge due to their slow growth rate and limited tendency to form biofilms. In this study, AOA was embedded in small (~2.5 mm) and large (~4.7 mm) poly(vinyl alcohol) (PVA)—so...

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Autores principales:	Matthieu Landreau, HeeJun You, David A. Stahl, Mari K. H. Winkler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/559dc8b74565453ca80cd143f57b233b
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id	oai:doaj.org-article:559dc8b74565453ca80cd143f57b233b
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spelling	oai:doaj.org-article:559dc8b74565453ca80cd143f57b233b2021-12-02T17:19:17ZImmobilization of active ammonia-oxidizing archaea in hydrogel beads10.1038/s41545-021-00134-12059-7037https://doaj.org/article/559dc8b74565453ca80cd143f57b233b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41545-021-00134-1https://doaj.org/toc/2059-7037Abstract Ammonia-oxidizing archaea (AOA) are major players in the nitrogen cycle but their cultivation represents a major challenge due to their slow growth rate and limited tendency to form biofilms. In this study, AOA was embedded in small (~2.5 mm) and large (~4.7 mm) poly(vinyl alcohol) (PVA)—sodium alginate (SA) hydrogel beads cross-linked with four agents (calcium, barium, light, or sulfate) to compare the differences in activity, the diffusivity of nitrogen species (NH4 +, NO2 −, and NO3 −), and polymer leakage in batch systems over time. Sulfate-bound PVA-SA beads were the most stable, releasing the lowest amount of polymer without shrinking. Diffusion coefficients were found to be 2 to 3 times higher in hydrogels than in granules, with ammonium diffusivity being ca. 35% greater than nitrite and nitrate. Despite a longer lag phase in small beads, embedded AOA sustained a high per volume rate of ammonia oxidation compatible with applications in research and wastewater treatment.Matthieu LandreauHeeJun YouDavid A. StahlMari K. H. WinklerNature PortfolioarticleWater supply for domestic and industrial purposesTD201-500ENnpj Clean Water, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Water supply for domestic and industrial purposes TD201-500
spellingShingle	Water supply for domestic and industrial purposes TD201-500 Matthieu Landreau HeeJun You David A. Stahl Mari K. H. Winkler Immobilization of active ammonia-oxidizing archaea in hydrogel beads
description	Abstract Ammonia-oxidizing archaea (AOA) are major players in the nitrogen cycle but their cultivation represents a major challenge due to their slow growth rate and limited tendency to form biofilms. In this study, AOA was embedded in small (~2.5 mm) and large (~4.7 mm) poly(vinyl alcohol) (PVA)—sodium alginate (SA) hydrogel beads cross-linked with four agents (calcium, barium, light, or sulfate) to compare the differences in activity, the diffusivity of nitrogen species (NH4 +, NO2 −, and NO3 −), and polymer leakage in batch systems over time. Sulfate-bound PVA-SA beads were the most stable, releasing the lowest amount of polymer without shrinking. Diffusion coefficients were found to be 2 to 3 times higher in hydrogels than in granules, with ammonium diffusivity being ca. 35% greater than nitrite and nitrate. Despite a longer lag phase in small beads, embedded AOA sustained a high per volume rate of ammonia oxidation compatible with applications in research and wastewater treatment.
format	article
author	Matthieu Landreau HeeJun You David A. Stahl Mari K. H. Winkler
author_facet	Matthieu Landreau HeeJun You David A. Stahl Mari K. H. Winkler
author_sort	Matthieu Landreau
title	Immobilization of active ammonia-oxidizing archaea in hydrogel beads
title_short	Immobilization of active ammonia-oxidizing archaea in hydrogel beads
title_full	Immobilization of active ammonia-oxidizing archaea in hydrogel beads
title_fullStr	Immobilization of active ammonia-oxidizing archaea in hydrogel beads
title_full_unstemmed	Immobilization of active ammonia-oxidizing archaea in hydrogel beads
title_sort	immobilization of active ammonia-oxidizing archaea in hydrogel beads
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/559dc8b74565453ca80cd143f57b233b
work_keys_str_mv	AT matthieulandreau immobilizationofactiveammoniaoxidizingarchaeainhydrogelbeads AT heejunyou immobilizationofactiveammoniaoxidizingarchaeainhydrogelbeads AT davidastahl immobilizationofactiveammoniaoxidizingarchaeainhydrogelbeads AT marikhwinkler immobilizationofactiveammoniaoxidizingarchaeainhydrogelbeads
_version_	1718381026387951616

Immobilization of active ammonia-oxidizing archaea in hydrogel beads

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