Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats

BACKGROUND AND OBJECTIVE: Ribes khorassanicum has beneficial cardiovascular effects in normotensive and hypertensive animals. For further investigation, the effect of three fractions of aqueous (polar), ethyl acetate (polar and non-polar) and n-hexane (non-polar) of the plant s hydroalcoholic extrac...

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Autores principales:	R Mohebbati, Y Kamkar-Del, I Hamounpeyma, V Alikhani, H Darroudi, MN Shafei
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2021
Materias:	ribes khorassanicum n-hexane fraction blood pressure heart rate. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/559e26d4d4e7459e8e32d374ba19e778
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spelling	oai:doaj.org-article:559e26d4d4e7459e8e32d374ba19e7782021-11-29T07:40:49ZComparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats1561-41072251-7170https://doaj.org/article/559e26d4d4e7459e8e32d374ba19e7782021-03-01T00:00:00Zhttp://jbums.org/article-1-9304-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Ribes khorassanicum has beneficial cardiovascular effects in normotensive and hypertensive animals. For further investigation, the effect of three fractions of aqueous (polar), ethyl acetate (polar and non-polar) and n-hexane (non-polar) of the plant s hydroalcoholic extract on cardiovascular parameters in normotensive rats was compared with its total extract. METHODS: In this experimental study, 42 rats were divided into 7 groups (6 in each group): control, three doses of total extract 4, 12 and 24 mg/kg and three fractions of aqueous, ethyl acetate and n-hexane (20 mg/kg). Femoral artery cannulation and heart rate (HR), mean arterial pressure (MAP) and systolic blood pressure (SBP) were recorded throughout the experiment and then the changes (Δ: difference before and after injection) were calculated and statistically analyzed. FINDINGS: 24 mg/kg of the extract significantly increased ΔHR (117.5±11.6 beats per minute) compared to control (5.3±2.7, p<0.001) and 12 mg/kg of the extract significantly decreased ΔMAP (-15.1±2.8 mm Hg) and ΔSBP (-18.4±3 mm Hg) compared to the control (p<0.05). The fractions increased ΔHR, which was not significant but was significant compared to 24 mg/kg of the extract (p<0.05). Furthermore, the increase of ΔMAP and ΔSBP in n-hexane fraction (19.45±3.1 mm Hg and 34.6±11.5 mm Hg, respectively) was significant compared to the control and 12 mg/kg of the extract (p<0.01 to p<0.05). CONCLUSION: The active ingredients in the cardiovascular effects of Ribes khorassanicum extract are spread in all fractions and the total extract has a better effect than each of the fractions alone due to its various non-polar and polar compounds as well as the synergistic effect.R MohebbatiY Kamkar-DelI HamounpeymaV AlikhaniH DarroudiMN ShafeiBabol University of Medical Sciencesarticleribes khorassanicumn-hexane fractionblood pressureheart rate.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 9-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	ribes khorassanicum n-hexane fraction blood pressure heart rate. Medicine R Medicine (General) R5-920
spellingShingle	ribes khorassanicum n-hexane fraction blood pressure heart rate. Medicine R Medicine (General) R5-920 R Mohebbati Y Kamkar-Del I Hamounpeyma V Alikhani H Darroudi MN Shafei Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
description	BACKGROUND AND OBJECTIVE: Ribes khorassanicum has beneficial cardiovascular effects in normotensive and hypertensive animals. For further investigation, the effect of three fractions of aqueous (polar), ethyl acetate (polar and non-polar) and n-hexane (non-polar) of the plant s hydroalcoholic extract on cardiovascular parameters in normotensive rats was compared with its total extract. METHODS: In this experimental study, 42 rats were divided into 7 groups (6 in each group): control, three doses of total extract 4, 12 and 24 mg/kg and three fractions of aqueous, ethyl acetate and n-hexane (20 mg/kg). Femoral artery cannulation and heart rate (HR), mean arterial pressure (MAP) and systolic blood pressure (SBP) were recorded throughout the experiment and then the changes (Δ: difference before and after injection) were calculated and statistically analyzed. FINDINGS: 24 mg/kg of the extract significantly increased ΔHR (117.5±11.6 beats per minute) compared to control (5.3±2.7, p<0.001) and 12 mg/kg of the extract significantly decreased ΔMAP (-15.1±2.8 mm Hg) and ΔSBP (-18.4±3 mm Hg) compared to the control (p<0.05). The fractions increased ΔHR, which was not significant but was significant compared to 24 mg/kg of the extract (p<0.05). Furthermore, the increase of ΔMAP and ΔSBP in n-hexane fraction (19.45±3.1 mm Hg and 34.6±11.5 mm Hg, respectively) was significant compared to the control and 12 mg/kg of the extract (p<0.01 to p<0.05). CONCLUSION: The active ingredients in the cardiovascular effects of Ribes khorassanicum extract are spread in all fractions and the total extract has a better effect than each of the fractions alone due to its various non-polar and polar compounds as well as the synergistic effect.
format	article
author	R Mohebbati Y Kamkar-Del I Hamounpeyma V Alikhani H Darroudi MN Shafei
author_facet	R Mohebbati Y Kamkar-Del I Hamounpeyma V Alikhani H Darroudi MN Shafei
author_sort	R Mohebbati
title	Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
title_short	Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
title_full	Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
title_fullStr	Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
title_full_unstemmed	Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats
title_sort	comparison of cardiovascular effects of ribes khorassanicum fractions with its total extract in normotensive rats
publisher	Babol University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/559e26d4d4e7459e8e32d374ba19e778
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Comparison of Cardiovascular Effects of Ribes Khorassanicum Fractions with Its Total Extract in Normotensive Rats

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