ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of co...

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Autores principales: N. A. Sharapova, M. N. Kireyev, E. G. Abramova, S. V. Generalov, Zh. V. Matveyeva, I. M. Zhulidov, N. E. Tereshkina, E. A. Mikheyeva, L. V. Savitskaya, A. V. Gayeva, A. K. Nikiforov
Formato: article
Lenguaje:RU
Publicado: Scientific Сentre for Family Health and Human Reproduction Problems 2012
Materias:
glicoproteid
fixed rabies virus
diagnosticum
Science
Q
Acceso en línea:https://doaj.org/article/55a2c7b860f944e391c3c15343a8ce95
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Sumario:Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay.

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